LOCUS       BBa_K775010             2597 bp    DNA     linear       30-Apr-2024
DEFINITION  Isoamylacetate Chimeric GPCR with Fus1-EGFP reporter (2)
ACCESSION   BBa_K775010
VERSION     BBa_K775010.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=FUS1 promoter
     misc_feature    201..206
                     /label=BamHi restriction site
     RBS             207..212
                     /label=Kozak sequence
     CDS             213..936
                     /label=EGFP
     misc_feature    937..942
                     /label=NdeI restriction site
     misc_feature    942..1166
                     /label=ADH1 terminator
     misc_feature    1..1166
                     /label=BBa_K775004
     CDS             1175..2597
                     /label=BBa_K775001
ORIGIN
        1 ttttggtgcg atgatgaaac aaacatgaaa cgtcagtaat ttgaaacaaa taacgtaatt
       61 ctcgggattg gttttattta aatgacaatg taagagtggc tttgtaaggt atgtgttgct
      121 cttaaaatat ttggatacga catcctttat ctttttcctt taagagcagg atataagcca
      181 tcaagtttct gaaaatcaaa ggatccaaaa aaatggtgag caagggcgag gagctgttca
      241 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      301 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      361 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      421 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      481 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      541 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      601 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      661 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      721 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      781 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
      841 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      901 tcactctcgg catggacgag ctgtacaagt aataacatat gtaataagcg aatttcttat
      961 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1021 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     1081 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     1141 cgagcaaatg cctgcaaatc gctccctact agagcggtag gtattgattg taattctgta
     1201 aatctatttc ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa
     1261 aacaccagaa cttagtttcg acggatggat ccaaaaaaat ggactacaag gacgacgacg
     1321 acaaggagcg aaggaaccac agtgggagag tgagtgaatt tgtgttgctg ggtttcccag
     1381 ctcctgcccc actgcgagta ctactatttt tcctttctct tctggcctat gtgttggtgt
     1441 tgactgaaaa catgctcatc attatagcaa ttaggaacca cccaaccctc cacaaaccca
     1501 tgtatcacct ggacactcga ctccacatcc ccatgtactt ttttctcagc cacttgtcct
     1561 ttgtggatgc ctgctcctct tctgtcataa gtcctaagat gttgtctgac atgtttgtgg
     1621 ataaaaaggt gatctccttc ttaggttgtg ctatccagct ttgtttattt agccagtttg
     1681 tagtgacaga atgtttcctc ctggcctcca tggcttatga tcggtatgtt gccatctgta
     1741 agcctttgct gtatactctc atcatgtccc agcgagtctg tgtacagtta gtgattggcc
     1801 cttacagcat aggctttgta agcactatgg tccatattat ttctgcattt gtccttccat
     1861 actgtggtcc aaatctcatc aatcactttt tctgtgacct tcttcctgtc ctctcactgg
     1921 catgtgcaaa tactcagatg aaaaaacgtt tgcttttcat cgtggctggg attctaggtg
     1981 tattcagtgg tataatcata ttggtgtcct atgtttacat tgccatcacc atcctaaaga
     2041 tcagttctgc tgatgggagg cgcaaagcct tctccacctg ctcttcacac ctgacagcag
     2101 tctctattct ttacgggact ctcttcttta tctatgtacg tccaagctcc agcttctctc
     2161 tggatatcaa taaggttgtg tcactatttt ataccactgt tatccctatg ttgaacccat
     2221 tcatttatag cctgtactgc ttgcgcaacc aagatgtcaa aagagcgcta cgtcgcacgc
     2281 tgcacctggc ccaggaccag gaggccaata ccaacaaagg cagcaaaatt ggttaataac
     2341 atatgtcatg taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct
     2401 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
     2461 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
     2521 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa
     2581 ggctttaatt tgcggcc
//