BBa_K775010 1 BBa_K775010 Isoamylacetate Chimeric GPCR with Fus1-EGFP reporter (2) 2012-09-23T11:00:00Z 2015-05-08T01:13:16Z - Released HQ 2013 - false false _1027_ 0 12035 9 In stock false - false Sietske Grijseels component2195597 1 BBa_K775001 component2195596 1 BBa_K775004 annotation2195596 1 BBa_K775004 range2195596 1 1 1166 annotation2195597 1 BBa_K775001 range2195597 1 1175 2597 BBa_K775004 1 BBa_K775004 GPCR reporter FUS1-EGFP 2012-09-20T11:00:00Z 2015-05-08T01:13:16Z The sequence of the Fus1 promoter comes from S. cerevisiae This reporter can be used in combination with the expression of a G-protein coupled receptor in yeast. The Fus1 promoter is a promoter in the mating pathway (a G-protein coupled receptor pathway. If the receptor of this pathway is activated by the pheromone of the opposite mating type the mating pathway is activated and the Fus1 promoter is switched on. false false _1027_ 0 12035 9 It's complicated true - false Sietske Grijseels annotation2191555 1 ADH1 terminator range2191555 1 942 1166 annotation2191553 1 EGFP range2191553 1 213 936 annotation2191551 1 BamHi restriction site range2191551 1 201 206 annotation2191554 1 NdeI restriction site range2191554 1 937 942 annotation2191550 1 FUS1 promoter range2191550 1 1 200 annotation2191552 1 Kozak sequence range2191552 1 207 212 BBa_K775001 1 BBa_K775001 RI7-Olfr154 Isoamylacetate chimeric GPCR 2012-09-04T11:00:00Z 2015-05-08T01:13:15Z N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: Olfr154 NM_013728, Mus musculus, (mouse) C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Released HQ 2013 BBa_K775001 encodes a chimeric isoamylacetate-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mouse Olfr154, flanked by the N- and C- terminals of the rat OR RI7. false false _1027_ 0 12035 9 In stock false - false Sietske Grijseels BBa_K775001_sequence 1 cggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggatggatccaaaaaaatggactacaaggacgacgacgacaaggagcgaaggaaccacagtgggagagtgagtgaatttgtgttgctgggtttcccagctcctgccccactgcgagtactactatttttcctttctcttctggcctatgtgttggtgttgactgaaaacatgctcatcattatagcaattaggaaccacccaaccctccacaaacccatgtatcacctggacactcgactccacatccccatgtacttttttctcagccacttgtcctttgtggatgcctgctcctcttctgtcataagtcctaagatgttgtctgacatgtttgtggataaaaaggtgatctccttcttaggttgtgctatccagctttgtttatttagccagtttgtagtgacagaatgtttcctcctggcctccatggcttatgatcggtatgttgccatctgtaagcctttgctgtatactctcatcatgtcccagcgagtctgtgtacagttagtgattggcccttacagcataggctttgtaagcactatggtccatattatttctgcatttgtccttccatactgtggtccaaatctcatcaatcactttttctgtgaccttcttcctgtcctctcactggcatgtgcaaatactcagatgaaaaaacgtttgcttttcatcgtggctgggattctaggtgtattcagtggtataatcatattggtgtcctatgtttacattgccatcaccatcctaaagatcagttctgctgatgggaggcgcaaagccttctccacctgctcttcacacctgacagcagtctctattctttacgggactctcttctttatctatgtacgtccaagctccagcttctctctggatatcaataaggttgtgtcactattttataccactgttatccctatgttgaacccattcatttatagcctgtactgcttgcgcaaccaagatgtcaaaagagcgctacgtcgcacgctgcacctggcccaggaccaggaggccaataccaacaaaggcagcaaaattggttaataacatatgtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcc BBa_K775004_sequence 1 ttttggtgcgatgatgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaggatccaaaaaaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataacatatgtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K775010_sequence 1 ttttggtgcgatgatgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaggatccaaaaaaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataacatatgtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccctactagagcggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggatggatccaaaaaaatggactacaaggacgacgacgacaaggagcgaaggaaccacagtgggagagtgagtgaatttgtgttgctgggtttcccagctcctgccccactgcgagtactactatttttcctttctcttctggcctatgtgttggtgttgactgaaaacatgctcatcattatagcaattaggaaccacccaaccctccacaaacccatgtatcacctggacactcgactccacatccccatgtacttttttctcagccacttgtcctttgtggatgcctgctcctcttctgtcataagtcctaagatgttgtctgacatgtttgtggataaaaaggtgatctccttcttaggttgtgctatccagctttgtttatttagccagtttgtagtgacagaatgtttcctcctggcctccatggcttatgatcggtatgttgccatctgtaagcctttgctgtatactctcatcatgtcccagcgagtctgtgtacagttagtgattggcccttacagcataggctttgtaagcactatggtccatattatttctgcatttgtccttccatactgtggtccaaatctcatcaatcactttttctgtgaccttcttcctgtcctctcactggcatgtgcaaatactcagatgaaaaaacgtttgcttttcatcgtggctgggattctaggtgtattcagtggtataatcatattggtgtcctatgtttacattgccatcaccatcctaaagatcagttctgctgatgggaggcgcaaagccttctccacctgctcttcacacctgacagcagtctctattctttacgggactctcttctttatctatgtacgtccaagctccagcttctctctggatatcaataaggttgtgtcactattttataccactgttatccctatgttgaacccattcatttatagcctgtactgcttgcgcaaccaagatgtcaaaagagcgctacgtcgcacgctgcacctggcccaggaccaggaggccaataccaacaaaggcagcaaaattggttaataacatatgtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z