LOCUS       BBa_K775012             2618 bp    DNA     linear       22-Nov-2024
DEFINITION  diacetyl chimeric GPCR with Fus1-EGFP reporter (2)
ACCESSION   BBa_K775012
VERSION     BBa_K775012.1  
FEATURES             Location/Qualifiers
     CDS             1..1444
                     /label=BBa_K775003
     promoter        1453..1652
                     /label=FUS1 promoter
     misc_feature    1653..1658
                     /label=BamHi restriction site
     RBS             1659..1664
                     /label=Kozak sequence
     CDS             1665..2388
                     /label=EGFP
     misc_feature    2389..2394
                     /label=NdeI restriction site
     misc_feature    2394..2618
                     /label=ADH1 terminator
     misc_feature    1453..2618
                     /label=BBa_K775004
ORIGIN
        1 cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt
       61 atagttagtc ttttttttag ttttaaaaca ccagaactta gtttcgacgg atggatccaa
      121 aaaaatggag cgaaggaacc acagtgggag agtgagtgaa tttgtgttgc tgggtttccc
      181 agctcctgcc ccactgcgag tactactatt tttcctttct cttctggcct atgtgttggt
      241 gttgactgaa aacatgctca tcattatagc aattaggaac cacccaaccc tccacaaacc
      301 catgtatttt ttctcagtat tctcgatttt ttacgccatc atcgagttca tattacgacc
      361 tataatgcat attgagaaca ccactttctt tttgatctca aggaaaagat tcaactactc
      421 caccaaactt ggaaaaatca actctgcgtt ttactgtgct tgttttgcca ccagttttgt
      481 tgtctcagga gttcactttg tttatcgata ttttgcaact tgcaaaccga atctacttcg
      541 tttgttcaac ttgccaactc ttctactttg gccacttggt tgcagtgtac ccgtgacaat
      601 gtgggctagt gtctcatatt ttttgtatcc agataccgag tacacggaag cggctgtcac
      661 caatgtacta aataaccact ataactggat caaaaaggag aatgtatcgt acattgcata
      721 cgtctattac caatacgaaa acggagtaag gcatatctac ctcaaaaact tgcttggatg
      781 ctttgttcat tactttgtca tgtcgatgac gtttgttgtg atgttctact gcggatatgc
      841 cacgtggaaa actatgaatg aacacaagga tgtatctgat agaactcgag cgctacagaa
      901 acaacttttc aaagctttag ttcttcagac actcatccca actatcttca tgtacgcccc
      961 aactggagtc atgttcatcg caccgttttt tgacgtgaat ttgaatgcaa acgccaattt
     1021 cattgtgttt tgctcatttc tgtacccggg actcgatcca ctcattctga ttttgatcat
     1081 ttactgcttg cgcaaccaag atgtcaaaag agcgctacgt cgcacgctgc acctggccca
     1141 ggaccaggag gccaatacca acaaaggcag caaaattggt taataacata tgtcatgtaa
     1201 ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa
     1261 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
     1321 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
     1381 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
     1441 ggcctactag agttttggtg cgatgatgaa acaaacatga aacgtcagta atttgaaaca
     1501 aataacgtaa ttctcgggat tggttttatt taaatgacaa tgtaagagtg gctttgtaag
     1561 gtatgtgttg ctcttaaaat atttggatac gacatccttt atctttttcc tttaagagca
     1621 ggatataagc catcaagttt ctgaaaatca aaggatccaa aaaaatggtg agcaagggcg
     1681 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1741 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1801 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1861 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1921 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1981 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2041 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2101 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2161 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2221 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2281 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     2341 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataacat atgtaataag
     2401 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     2461 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     2521 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct
     2581 ctaccggcat gccgagcaaa tgcctgcaaa tcgctccc
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