BBa_K775004 1 BBa_K775004 GPCR reporter FUS1-EGFP 2012-09-20T11:00:00Z 2015-05-08T01:13:16Z The sequence of the Fus1 promoter comes from S. cerevisiae This reporter can be used in combination with the expression of a G-protein coupled receptor in yeast. The Fus1 promoter is a promoter in the mating pathway (a G-protein coupled receptor pathway. If the receptor of this pathway is activated by the pheromone of the opposite mating type the mating pathway is activated and the Fus1 promoter is switched on. false false _1027_ 0 12035 9 It's complicated true - false Sietske Grijseels annotation2191551 1 BamHi restriction site range2191551 1 201 206 annotation2191550 1 FUS1 promoter range2191550 1 1 200 annotation2191552 1 Kozak sequence range2191552 1 207 212 annotation2191554 1 NdeI restriction site range2191554 1 937 942 annotation2191553 1 EGFP range2191553 1 213 936 annotation2191555 1 ADH1 terminator range2191555 1 942 1166 BBa_K775012 1 BBa_K775012 diacetyl chimeric GPCR with Fus1-EGFP reporter (2) 2012-09-23T11:00:00Z 2015-05-08T01:13:16Z - - false false _1027_ 0 12035 9 It's complicated false - false Sietske Grijseels component2203284 1 BBa_K775003 component2203291 1 BBa_K775004 annotation2203291 1 BBa_K775004 range2203291 1 1453 2618 annotation2203284 1 BBa_K775003 range2203284 1 1 1444 BBa_K775003 1 BBa_K775003 RI7-odr10 (diacetyl) chimeric GPCR with promoter and terminator 2012-09-04T11:00:00Z 2015-05-08T01:13:16Z N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: C. elegans 7-transmembrane receptor ODR-10 U49449.1 C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Released HQ 2013 BBa_K775003 encodes a chimeric diacetyl-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the C. elegans 7-transmembrane receptor ODR-10, flanked by the N- and C- terminals of the rat OR RI7. false false _1027_ 0 12035 9 In stock false - false Sietske Grijseels BBa_K775004_sequence 1 ttttggtgcgatgatgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaggatccaaaaaaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataacatatgtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K775012_sequence 1 cggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggatggatccaaaaaaatggagcgaaggaaccacagtgggagagtgagtgaatttgtgttgctgggtttcccagctcctgccccactgcgagtactactatttttcctttctcttctggcctatgtgttggtgttgactgaaaacatgctcatcattatagcaattaggaaccacccaaccctccacaaacccatgtattttttctcagtattctcgattttttacgccatcatcgagttcatattacgacctataatgcatattgagaacaccactttctttttgatctcaaggaaaagattcaactactccaccaaacttggaaaaatcaactctgcgttttactgtgcttgttttgccaccagttttgttgtctcaggagttcactttgtttatcgatattttgcaacttgcaaaccgaatctacttcgtttgttcaacttgccaactcttctactttggccacttggttgcagtgtacccgtgacaatgtgggctagtgtctcatattttttgtatccagataccgagtacacggaagcggctgtcaccaatgtactaaataaccactataactggatcaaaaaggagaatgtatcgtacattgcatacgtctattaccaatacgaaaacggagtaaggcatatctacctcaaaaacttgcttggatgctttgttcattactttgtcatgtcgatgacgtttgttgtgatgttctactgcggatatgccacgtggaaaactatgaatgaacacaaggatgtatctgatagaactcgagcgctacagaaacaacttttcaaagctttagttcttcagacactcatcccaactatcttcatgtacgccccaactggagtcatgttcatcgcaccgttttttgacgtgaatttgaatgcaaacgccaatttcattgtgttttgctcatttctgtacccgggactcgatccactcattctgattttgatcatttactgcttgcgcaaccaagatgtcaaaagagcgctacgtcgcacgctgcacctggcccaggaccaggaggccaataccaacaaaggcagcaaaattggttaataacatatgtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcctactagagttttggtgcgatgatgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaggatccaaaaaaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataacatatgtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K775003_sequence 1 cggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagttttaaaacaccagaacttagtttcgacggatggatccaaaaaaatggagcgaaggaaccacagtgggagagtgagtgaatttgtgttgctgggtttcccagctcctgccccactgcgagtactactatttttcctttctcttctggcctatgtgttggtgttgactgaaaacatgctcatcattatagcaattaggaaccacccaaccctccacaaacccatgtattttttctcagtattctcgattttttacgccatcatcgagttcatattacgacctataatgcatattgagaacaccactttctttttgatctcaaggaaaagattcaactactccaccaaacttggaaaaatcaactctgcgttttactgtgcttgttttgccaccagttttgttgtctcaggagttcactttgtttatcgatattttgcaacttgcaaaccgaatctacttcgtttgttcaacttgccaactcttctactttggccacttggttgcagtgtacccgtgacaatgtgggctagtgtctcatattttttgtatccagataccgagtacacggaagcggctgtcaccaatgtactaaataaccactataactggatcaaaaaggagaatgtatcgtacattgcatacgtctattaccaatacgaaaacggagtaaggcatatctacctcaaaaacttgcttggatgctttgttcattactttgtcatgtcgatgacgtttgttgtgatgttctactgcggatatgccacgtggaaaactatgaatgaacacaaggatgtatctgatagaactcgagcgctacagaaacaacttttcaaagctttagttcttcagacactcatcccaactatcttcatgtacgccccaactggagtcatgttcatcgcaccgttttttgacgtgaatttgaatgcaaacgccaatttcattgtgttttgctcatttctgtacccgggactcgatccactcattctgattttgatcatttactgcttgcgcaaccaagatgtcaaaagagcgctacgtcgcacgctgcacctggcccaggaccaggaggccaataccaacaaaggcagcaaaattggttaataacatatgtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z