BBa_K777100 1 BBa_K777100 flhDC 2012-09-14T11:00:00Z 2015-05-08T01:13:16Z The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. false false _1029_ 0 12106 9 In stock false The PstI restriction site was mutated to CTGCGG using the reverse primer. false Team Goettingen annotation2183218 1 TGA stop codon range2183218 1 349 351 annotation2183224 1 TAA stop codon range2183224 1 867 869 annotation2183225 1 PstI site removed range2183225 1 839 839 annotation2183217 1 FlhD coding region range2183217 1 1 351 annotation2183220 1 ATG start codon range2183220 1 354 356 annotation2183216 1 ATG start codon range2183216 1 1 3 annotation2183222 1 FlhC coding region range2183222 1 354 869 BBa_K777100_sequence 1 atgcatacctccgagttgctgaaacacatttatgacatcaacttgtcatatttactacttgcacagcgtttgattgttcaggacaaagcgtccgctatgtttcgtctcggcataaatgaagaaatggcgacaacgttagcggcactgactcttccgcaaatggttaagctggcagaaaccaatcaactggtttgtcacttccgttttgacagccaccagacgattactcagttgacgcaagattcccgcgttgacgatctccagcaaattcataccggcatcatgctctcaacacgcttgctgaatgatgttaatcagcctgaagaagcgctgcgcaagaaaagggcctgatcatgagtgaaaaaagcattgttcaggaagcgcgggatattcagctggcaatggaattgatcaccctgggcgctcgtttgcagatgctggaaagcgaaacacagttaagtcgcggacgcctgataaaactttataaagaactgcgcggaagcccaccgccgaaaggcatgctgccattctcaaccgactggtttatgacctgggaacaaaacgttcatgcttcgatgttctgtaatgcatggcagtttttactgaaaaccggtttgtgtaatggcgtcgatgcggtgatcaaagcctaccgtttataccttgaacagtgcccacaagcagaagaaggaccactgctggcattaacccgtgcctggacattggtgcggtttgttgaaagtggattactgcaactttccagctgcaactttccagctgcaactgctgcggcggcaattttattacccacgctcaccagcctgttggcagctttgcctgcggcttatgtcaaccgccatcccgggcagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z