BBa_K777101 1 BBa_K777101 flhDC operon under the control of constitutive promoter J23100 2012-09-18T11:00:00Z 2015-05-08T01:13:16Z *The part was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). *J23100 information was from partsregistry and physical DNA from the 2012 distribution kit. The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. Here we used a set of 8 constitutive promoters from the 2006 Berkeley group to test how different levels of constitutive ''flhDC'' expression affect motility in ''E. coli''. false false _1029_ 0 12106 9 In stock false *The PstI restriction site in the ''flhDC'' sequence was mutated to CTGCGG using the reverse primer. *''flhDC'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-actgaattcgcggccgcttctagatgcatacctccgagttgctg-3'<br> **Rev: 5'-tcctgcagcggccgctactagttactgcccgggatggcggttgacataagcCgcaggcaaagctgccaacag-3' <br>(the capitalized "C" induces the mutation for removal of the PstI site) false Team Goettingen component2186537 1 BBa_K777100 component2186529 1 BBa_J23100 annotation2186537 1 BBa_K777100 range2186537 1 42 910 annotation2186529 1 BBa_J23100 range2186529 1 1 35 BBa_K777100 1 BBa_K777100 flhDC 2012-09-14T11:00:00Z 2015-05-08T01:13:16Z The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). The flhDC operon is the master regulator of motility and chemotaxis in E. coli. FlhDC forms heterotetramers and activates class II operons in concert with sigma factor 70. Among the gene products of class II operons are several components of the flagellum and the alternative sigma factor FliA which is essential for the transcription of class III genes. false false _1029_ 0 12106 9 In stock false The PstI restriction site was mutated to CTGCGG using the reverse primer. false Team Goettingen annotation2183225 1 PstI site removed range2183225 1 839 839 annotation2183220 1 ATG start codon range2183220 1 354 356 annotation2183216 1 ATG start codon range2183216 1 1 3 annotation2183222 1 FlhC coding region range2183222 1 354 869 annotation2183218 1 TGA stop codon range2183218 1 349 351 annotation2183224 1 TAA stop codon range2183224 1 867 869 annotation2183217 1 FlhD coding region range2183217 1 1 351 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K777100_sequence 1 atgcatacctccgagttgctgaaacacatttatgacatcaacttgtcatatttactacttgcacagcgtttgattgttcaggacaaagcgtccgctatgtttcgtctcggcataaatgaagaaatggcgacaacgttagcggcactgactcttccgcaaatggttaagctggcagaaaccaatcaactggtttgtcacttccgttttgacagccaccagacgattactcagttgacgcaagattcccgcgttgacgatctccagcaaattcataccggcatcatgctctcaacacgcttgctgaatgatgttaatcagcctgaagaagcgctgcgcaagaaaagggcctgatcatgagtgaaaaaagcattgttcaggaagcgcgggatattcagctggcaatggaattgatcaccctgggcgctcgtttgcagatgctggaaagcgaaacacagttaagtcgcggacgcctgataaaactttataaagaactgcgcggaagcccaccgccgaaaggcatgctgccattctcaaccgactggtttatgacctgggaacaaaacgttcatgcttcgatgttctgtaatgcatggcagtttttactgaaaaccggtttgtgtaatggcgtcgatgcggtgatcaaagcctaccgtttataccttgaacagtgcccacaagcagaagaaggaccactgctggcattaacccgtgcctggacattggtgcggtttgttgaaagtggattactgcaactttccagctgcaactttccagctgcaactgctgcggcggcaattttattacccacgctcaccagcctgttggcagctttgcctgcggcttatgtcaaccgccatcccgggcagtaa BBa_K777101_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagatgcatacctccgagttgctgaaacacatttatgacatcaacttgtcatatttactacttgcacagcgtttgattgttcaggacaaagcgtccgctatgtttcgtctcggcataaatgaagaaatggcgacaacgttagcggcactgactcttccgcaaatggttaagctggcagaaaccaatcaactggtttgtcacttccgttttgacagccaccagacgattactcagttgacgcaagattcccgcgttgacgatctccagcaaattcataccggcatcatgctctcaacacgcttgctgaatgatgttaatcagcctgaagaagcgctgcgcaagaaaagggcctgatcatgagtgaaaaaagcattgttcaggaagcgcgggatattcagctggcaatggaattgatcaccctgggcgctcgtttgcagatgctggaaagcgaaacacagttaagtcgcggacgcctgataaaactttataaagaactgcgcggaagcccaccgccgaaaggcatgctgccattctcaaccgactggtttatgacctgggaacaaaacgttcatgcttcgatgttctgtaatgcatggcagtttttactgaaaaccggtttgtgtaatggcgtcgatgcggtgatcaaagcctaccgtttataccttgaacagtgcccacaagcagaagaaggaccactgctggcattaacccgtgcctggacattggtgcggtttgttgaaagtggattactgcaactttccagctgcaactttccagctgcaactgctgcggcggcaattttattacccacgctcaccagcctgttggcagctttgcctgcggcttatgtcaaccgccatcccgggcagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z