BBa_K777113 1 BBa_K777113 motA 2012-09-16T11:00:00Z 2015-05-08T01:13:16Z * The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1). Released HQ 2013 The motA gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. false false _1029_ 0 12106 9 In stock false *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGACCGGTGGAAGCCTTGG <br> **Rev: GTTTCTTCCTGCAGCGGCCGCTACTAGTATCATGCTTCCTCGGTTGTCG false Team Goettingen annotation2184142 1 MotA coding sequence range2184142 1 1 831 annotation2184143 1 start range2184143 1 1 1 annotation2184144 1 stop range2184144 1 831 831 BBa_K777114 1 BBa_K777114 motA under the control of constitutive promoter J23100 2012-09-20T11:00:00Z 2015-05-08T01:13:16Z * ''motA'' was amplified from genomic DNA of ''E. coli'' str. K-12 substr. DH10B, complete genome (CP000948.1). * J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit. The ''motA'' gene codes for a part of the flagellar motor complex. MotA proteins represent the proton conducting component of this molecular machine. Thus it is necessary for flagellar rotation. <br> Here we used 3 different constitutive [http://partsregistry.org/Part:BBa_J23100 Anderson promoters] from the 2006 Berkeley group to test how different levels of constitutive ''motA'' expression affect the motility or flagellum size of ''E. coli''. false false _1029_ 0 12106 9 Not in stock false *''motA'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers: **Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgaccggtggaagccttgg-3' **Rev: 5'-gtttcttcctgcagcggccgctactagtatcatgcttcctcggttgtcg-3' false Team Goettingen component2189707 1 BBa_K777113 component2189705 1 BBa_J23100 annotation2189707 1 BBa_K777113 range2189707 1 42 872 annotation2189705 1 BBa_J23100 range2189705 1 1 35 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K777113_sequence 1 atgaccggtggaagccttggagcactctatcaacccgctgaactggtgattattgccggtgcagggattgggtcgtttatcgtcggcaataatggcaaagcgattaaaggcacgctgaaggcgctgccgttgctgtttcgtcgctccaaatacaccaaagcaatgtatatggatctgctggctctgctttatcggttgatggcgaaatcgcggcagatggggatgttttcgctggaacgtgatattgaaaatccccgtgagagcgagatcttcgccagctacccacgcatcctcgcggatagcgtcatgcttgattttatcgtcgattatctgcgcctgattatcagcggtcacatgaacaccttcgaaatcgaagctctgatggatgaagagattgagacgcacgaaagcgaggcagaagtcccggcgaacagtctggcgctggtcggggactcacttccggcgtttggtattgttgcggctgtaatgggggtcgttcacgcgttaggttcagccgatcgtcctgccgccgagctgggtgcgcttatcgcacatgcgatggtggggactttcctcggcattttattggcttacggatttatttccccattagcgactgttttacgtcagaaaagcgccgaaaccagcaaaatgatgcagtgcgtcaaagtcactctgctttctaatctgaacggttacgcaccgcctatcgccgttgagtttggtcgcaaaacgctctattccagcgaacgtccgtcgtttattgaactggaagagcatgtgcgtgcggtgaaaaatccgcaacaacagacgacaaccgaggaagcatga BBa_K777114_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagatgaccggtggaagccttggagcactctatcaacccgctgaactggtgattattgccggtgcagggattgggtcgtttatcgtcggcaataatggcaaagcgattaaaggcacgctgaaggcgctgccgttgctgtttcgtcgctccaaatacaccaaagcaatgtatatggatctgctggctctgctttatcggttgatggcgaaatcgcggcagatggggatgttttcgctggaacgtgatattgaaaatccccgtgagagcgagatcttcgccagctacccacgcatcctcgcggatagcgtcatgcttgattttatcgtcgattatctgcgcctgattatcagcggtcacatgaacaccttcgaaatcgaagctctgatggatgaagagattgagacgcacgaaagcgaggcagaagtcccggcgaacagtctggcgctggtcggggactcacttccggcgtttggtattgttgcggctgtaatgggggtcgttcacgcgttaggttcagccgatcgtcctgccgccgagctgggtgcgcttatcgcacatgcgatggtggggactttcctcggcattttattggcttacggatttatttccccattagcgactgttttacgtcagaaaagcgccgaaaccagcaaaatgatgcagtgcgtcaaagtcactctgctttctaatctgaacggttacgcaccgcctatcgccgttgagtttggtcgcaaaacgctctattccagcgaacgtccgtcgtttattgaactggaagagcatgtgcgtgcggtgaaaaatccgcaacaacagacgacaaccgaggaagcatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z