BBa_K777121
1
BBa_K777121
yhjH
2012-09-17T11:00:00Z
2015-05-08T01:13:17Z
The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
Released HQ 2013
The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in ''yhjH''-mutants.
false
true
_1029_
0
12106
9
In stock
true
* ''yhjH'' was amplified from genomic DNA of ''E. coli'' DH10B via PCR using the following primers:
** Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3'
** Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'
false
Flash Coli
annotation2184528
1
YhjH coding sequence
range2184528
1
1
768
annotation2184530
1
stop
range2184530
1
768
768
annotation2184529
1
start
range2184529
1
1
1
BBa_K777121_sequence
1
atgataaggcaggttatccagcgaataagcaaccctgaagcaagcatcgagagcttgcaggaacggcgtttttggttgcagtgtgagcgtgcttacacctggcagccgatctatcaaacatgcgggcggttaatggccgtggagctattaacggtggtcacgcatcccttgaacccttcgcaacgcctgccgccggatcgctattttactgaaatcaccgtcagccatcggatggaggttgtgaaagagcagattgatttgctggcgcaaaaagccgacttctttatagagcacggcctgctggcatcggtcaatattgatggccctacgctcatcgccctgcgtcagcaaccaaaaatcctgcgccagattgagcgtcttccctggctgcgtttcgaactggtggagcatatccgtctgccgaaagattcaacctttgcctcgatgtgtgaatttggcccgctgtggctggatgattttggtaccgggatggcaaatttctctgcgctaagtgaagtgcgttatgactacatcaaaatcgcgcgagaactgtttgtgatgctgcgtcagtcgccggaaggacgcacactcttttctcagcttttacatctaatgaatcgctattgtcgcggggtgattgtcgagggcgtagaaacgccggaagagtggcgtgatgttcagaactcgcccgcattcgccgcacaaggctggtttctttcacgcccggcaccgatagaaacgctgaatacggcggttctggcgctataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z