LOCUS       BBa_K777133             2937 bp    DNA     linear       22-Nov-2024
DEFINITION  Similar to pSB1C3, with RFP reporter
ACCESSION   BBa_K777133
VERSION     BBa_K777133.1  
FEATURES             Location/Qualifiers
     RBS             8..19
                     /label=RBS
     misc_feature    8..19
                     /label=BB0034
     CDS             26..703
                     /label=mRFP1
     misc_feature    26..703
                     /label=E1010
     misc_feature    1129..1735
                     /label=rep (pMB1)
     CDS             2028..2687
                     /label=Chloramphenicol resistance
     misc_feature    1144..1144
                     /label=ORI
     primer_bind     1023..1042
                     /label=VR primer binding site
     primer_bind     2799..2818
                     /label=VF2 primer binding site
     misc_feature    1..6
                     /label=SpeI
     misc_feature    884..889
                     /label=PstI
     misc_feature    2917..2922
                     /label=EcoRI
     misc_feature    2932..2937
                     /label=XbaI
ORIGIN
        1 actagtgaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
       61 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
      121 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
      181 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
      241 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
      301 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
      361 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
      421 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
      481 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
      541 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
      601 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
      661 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
      721 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      781 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      841 ccttcgggtg ggcctttctg cgtttatata ctagaagcgg ccgctgcagt ccggcaaaaa
      901 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
      961 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1141 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
     1921 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1981 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     2041 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2101 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     2161 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     2221 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     2281 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     2341 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     2401 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     2461 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     2521 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     2581 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2641 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     2701 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2761 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
     2821 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     2881 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctaga
//