LOCUS       BBa_K778002             2121 bp    DNA     linear       22-Dec-2024
DEFINITION  fhlA-E363G (engineered trascriptional activator fhlA with a 
            mutation)
ACCESSION   BBa_K778002
VERSION     BBa_K778002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1
                     /label=start
     misc_feature    1088..1088
                     /label=E363G mutation
     misc_feature    2077..2079
                     /label=stop
     misc_feature    1906..1906
                     /label=A to G (different from E. coli K-12 MG1655)
     CDS             1..2079
                     /label=FhlA-E363G
ORIGIN
        1 atgtcatata caccgatgag tgatctcgga caacaagggt tgttcgacat cactcggaca
       61 ctattgcagc agcccgatct ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt
      121 tctgcgctcg ccgacaacgc ggctattgtg ttgtggcaag cgcagactca acgtgcgtct
      181 tattacgcgt cgcgtgaaaa agacaccccc attaaatatg aagacgaaac tgttctggca
      241 cacggtccgg tacgcagcat tttgtcgcgc cctgatacgc tgcattgcag ttacgaagaa
      301 ttttgtgaaa cctggccgca gctggacgca ggtgggctat acccaaaatt tggtcactat
      361 tgcctgatgc cactggcggc ggaagggcat atttttggtg gctgtgaatt tattcgttat
      421 gacgatcgcc cctggagcga aaaagagttc aatcgtctgc aaacatttac gcagatcgtt
      481 tctgtcgtca ccgaacaaat ccagagccgc gtcgttaaca atgtcgacta tgagttgtta
      541 tgccgggaac gcgataactt ccgcatcctg gtcgccatca ccaacgcggt gctttcccgc
      601 ctggatatgg acgaactggt cagcgaagtc gccaaagaaa tccattacta tttcgacatt
      661 gacgatatca gtatcgtctt acgcagccac cgtaaaaaca aactcaacat ctactccact
      721 cactatcttg ataaacagca tcccgcccac gaacagagcg aagtcgatga agccggaacc
      781 ctcaccgaac gcgtgttcaa aagtaaagag atgctgctga tcaatctcca cgagcgggac
      841 gatttagccc cctatgaacg catgttgttc gacacctggg gcaaccagat tcaaaccttg
      901 tgcctgttac cgctgatgtc tggcgacacc atgctgggcg tgctgaaact ggcgcaatgc
      961 gaagagaaag tgtttaccac taccaatctg aatttactgc gccagattgc cgaacgtgtg
     1021 gcaatcgctg tcgataacgc cctcgcctat caggaaatcc atcgtctgaa agaacggctg
     1081 gttgatggaa acctcgccct gaccgagcag ctcaacaatg ttgatagtga atttggcgag
     1141 attattggcc gcagcgaagc catgtacagc gtgcttaaac aagttgaaat ggtggcgcaa
     1201 agtgacagta ccgtgctgat cctcggtgaa actggcacgg gtaaagagct gattgcccgt
     1261 gcgatccata atctcagtgg gcgtaataat cgccgcatgg tcaaaatgaa ctgcgcggcg
     1321 atgcctgccg gattgctgga aagcgatctg tttggtcatg agcgtggggc ttttaccggt
     1381 gccagcgccc agcgtatcgg tcgttttgaa ctggcggata aaagctccct gttcctcgac
     1441 gaagtgggcg atatgccact ggagttacag ccgaagttgc tgcgtgtatt gcaggaacag
     1501 gagtttgaac gtctcggcag caacaaaatc attcagacgg acgtgcgtct aatcgccgcg
     1561 actaaccgcg atctgaaaaa aatggtcgcc gaccgtgagt tccgtagcga tctctattac
     1621 cgcctgaacg tattcccgat tcacctgccg ccactacgcg agcgtccgga agatattccg
     1681 ctgctggcga aagcctttac cttcaaaatt gcccgtcgtc tggggcgcaa tatcgacagc
     1741 attcctgccg agacgctgcg caccttgagc aacatggagt ggccgggtaa cgtacgcgaa
     1801 ctggaaaacg tcattgagcg cgcggtattg ctaacacgcg gtaacgtgct gcaactgtca
     1861 ttgccagata ttgttttacc ggaacctgaa acgccgcctg ccgcagcggt tgtcgccctg
     1921 gagggcgaag atgaatatca gttgattgtg cgcgtgctga aagaaaccaa cggcgtggtt
     1981 gccgggccta aaggcgctgc gcaacgtctg gggctgaaac gcacgaccct gctgtcacgg
     2041 atgaagcggc tgggaattga taaatcggca ttgatttaac tgcaaattgc cggacagatc
     2101 tgcctgtccg gcatactatt c
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