LOCUS       BBa_K778005             2243 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS-fhlA363-d.term
ACCESSION   BBa_K778005
VERSION     BBa_K778005.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    20..20
                     /label=start
     misc_feature    1107..1107
                     /label=E363G mutation
     misc_feature    2096..2098
                     /label=stop
     misc_feature    1925..1925
                     /label=A to G (different from E. coli K-12 MG1655)
     CDS             20..2098
                     /label=FhlA-E363G
     CDS             20..2140
                     /label=BBa_K778002
     stem_loop       2156..2175
                     /label=T7 TE
     misc_feature    2182..2182
                     /label=stop
     polyA_site      2176..2189
                     /label=polya
     misc_feature    2149..2189
                     /label=BBa_B0012
     terminator      2149..2189
                     /label=BBa_B0012
     stem_loop       2210..2232
                     /label=stem_loop
     misc_feature    2198..2243
                     /label=BBa_B0011
     terminator      2198..2243
                     /label=BBa_B0011
     terminator      2149..2243
                     /label=BBa_B0014
ORIGIN
        1 tcacacagga aagtactaga tgtcatatac accgatgagt gatctcggac aacaagggtt
       61 gttcgacatc actcggacac tattgcagca gcccgatctg gcctcgctgt gtgaggctct
      121 ttcgcaactg gtaaagcgtt ctgcgctcgc cgacaacgcg gctattgtgt tgtggcaagc
      181 gcagactcaa cgtgcgtctt attacgcgtc gcgtgaaaaa gacaccccca ttaaatatga
      241 agacgaaact gttctggcac acggtccggt acgcagcatt ttgtcgcgcc ctgatacgct
      301 gcattgcagt tacgaagaat tttgtgaaac ctggccgcag ctggacgcag gtgggctata
      361 cccaaaattt ggtcactatt gcctgatgcc actggcggcg gaagggcata tttttggtgg
      421 ctgtgaattt attcgttatg acgatcgccc ctggagcgaa aaagagttca atcgtctgca
      481 aacatttacg cagatcgttt ctgtcgtcac cgaacaaatc cagagccgcg tcgttaacaa
      541 tgtcgactat gagttgttat gccgggaacg cgataacttc cgcatcctgg tcgccatcac
      601 caacgcggtg ctttcccgcc tggatatgga cgaactggtc agcgaagtcg ccaaagaaat
      661 ccattactat ttcgacattg acgatatcag tatcgtctta cgcagccacc gtaaaaacaa
      721 actcaacatc tactccactc actatcttga taaacagcat cccgcccacg aacagagcga
      781 agtcgatgaa gccggaaccc tcaccgaacg cgtgttcaaa agtaaagaga tgctgctgat
      841 caatctccac gagcgggacg atttagcccc ctatgaacgc atgttgttcg acacctgggg
      901 caaccagatt caaaccttgt gcctgttacc gctgatgtct ggcgacacca tgctgggcgt
      961 gctgaaactg gcgcaatgcg aagagaaagt gtttaccact accaatctga atttactgcg
     1021 ccagattgcc gaacgtgtgg caatcgctgt cgataacgcc ctcgcctatc aggaaatcca
     1081 tcgtctgaaa gaacggctgg ttgatggaaa cctcgccctg accgagcagc tcaacaatgt
     1141 tgatagtgaa tttggcgaga ttattggccg cagcgaagcc atgtacagcg tgcttaaaca
     1201 agttgaaatg gtggcgcaaa gtgacagtac cgtgctgatc ctcggtgaaa ctggcacggg
     1261 taaagagctg attgcccgtg cgatccataa tctcagtggg cgtaataatc gccgcatggt
     1321 caaaatgaac tgcgcggcga tgcctgccgg attgctggaa agcgatctgt ttggtcatga
     1381 gcgtggggct tttaccggtg ccagcgccca gcgtatcggt cgttttgaac tggcggataa
     1441 aagctccctg ttcctcgacg aagtgggcga tatgccactg gagttacagc cgaagttgct
     1501 gcgtgtattg caggaacagg agtttgaacg tctcggcagc aacaaaatca ttcagacgga
     1561 cgtgcgtcta atcgccgcga ctaaccgcga tctgaaaaaa atggtcgccg accgtgagtt
     1621 ccgtagcgat ctctattacc gcctgaacgt attcccgatt cacctgccgc cactacgcga
     1681 gcgtccggaa gatattccgc tgctggcgaa agcctttacc ttcaaaattg cccgtcgtct
     1741 ggggcgcaat atcgacagca ttcctgccga gacgctgcgc accttgagca acatggagtg
     1801 gccgggtaac gtacgcgaac tggaaaacgt cattgagcgc gcggtattgc taacacgcgg
     1861 taacgtgctg caactgtcat tgccagatat tgttttaccg gaacctgaaa cgccgcctgc
     1921 cgcagcggtt gtcgccctgg agggcgaaga tgaatatcag ttgattgtgc gcgtgctgaa
     1981 agaaaccaac ggcgtggttg ccgggcctaa aggcgctgcg caacgtctgg ggctgaaacg
     2041 cacgaccctg ctgtcacgga tgaagcggct gggaattgat aaatcggcat tgatttaact
     2101 gcaaattgcc ggacagatct gcctgtccgg catactattc tactagagtc acactggctc
     2161 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
     2221 ttaatccggc ttttttatta ttt
//