##gff-version 3
##sequence-region BBa_K778005 1 2243
BBa_K778005	.	terminator	2149	2243	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K778005	.	terminator	2149	2189	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K778005	.	engineered_region	2149	2189	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K778005	.	stop_codon	2182	2182	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K778005	.	polyA_site	2176	2189	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K778005	.	stem_loop	2156	2175	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K778005	.	terminator	2198	2243	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K778005	.	engineered_region	2198	2243	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K778005	.	stem_loop	2210	2232	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K778005	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K778005	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K778005	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K778005	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K778005	.	CDS	20	2140	.	+	0	ID=BBa_K778002;Name=BBa_K778002
BBa_K778005	.	sequence_alteration	1925	1925	.	+	0	ID=annotation2195764;Name=A to G (different from E. coli K-12 MG1655);Parent=BBa_K778002
BBa_K778005	.	CDS	20	2098	.	+	0	ID=annotation2201467;Name=FhlA-E363G;Parent=BBa_K778002
BBa_K778005	.	sequence_alteration	1107	1107	.	+	0	ID=annotation2194869;Name=E363G mutation;Parent=BBa_K778002
BBa_K778005	.	stop_codon	2096	2098	.	+	0	ID=annotation2194870;Name=stop;Parent=BBa_K778002
BBa_K778005	.	start_codon	20	20	.	+	0	ID=annotation2194868;Name=start;Parent=BBa_K778002
>BBa_K778005
tcacacaggaaagtactagatgtcatatacaccgatgagtgatctcggacaacaagggttgttcgacatcactcggaca
ctattgcagcagcccgatctggcctcgctgtgtgaggctctttcgcaactggtaaagcgttctgcgctcgccgacaacg
cggctattgtgttgtggcaagcgcagactcaacgtgcgtcttattacgcgtcgcgtgaaaaagacacccccattaaata
tgaagacgaaactgttctggcacacggtccggtacgcagcattttgtcgcgccctgatacgctgcattgcagttacgaa
gaattttgtgaaacctggccgcagctggacgcaggtgggctatacccaaaatttggtcactattgcctgatgccactgg
cggcggaagggcatatttttggtggctgtgaatttattcgttatgacgatcgcccctggagcgaaaaagagttcaatcg
tctgcaaacatttacgcagatcgtttctgtcgtcaccgaacaaatccagagccgcgtcgttaacaatgtcgactatgag
ttgttatgccgggaacgcgataacttccgcatcctggtcgccatcaccaacgcggtgctttcccgcctggatatggacg
aactggtcagcgaagtcgccaaagaaatccattactatttcgacattgacgatatcagtatcgtcttacgcagccaccg
taaaaacaaactcaacatctactccactcactatcttgataaacagcatcccgcccacgaacagagcgaagtcgatgaa
gccggaaccctcaccgaacgcgtgttcaaaagtaaagagatgctgctgatcaatctccacgagcgggacgatttagccc
cctatgaacgcatgttgttcgacacctggggcaaccagattcaaaccttgtgcctgttaccgctgatgtctggcgacac
catgctgggcgtgctgaaactggcgcaatgcgaagagaaagtgtttaccactaccaatctgaatttactgcgccagatt
gccgaacgtgtggcaatcgctgtcgataacgccctcgcctatcaggaaatccatcgtctgaaagaacggctggttgatg
gaaacctcgccctgaccgagcagctcaacaatgttgatagtgaatttggcgagattattggccgcagcgaagccatgta
cagcgtgcttaaacaagttgaaatggtggcgcaaagtgacagtaccgtgctgatcctcggtgaaactggcacgggtaaa
gagctgattgcccgtgcgatccataatctcagtgggcgtaataatcgccgcatggtcaaaatgaactgcgcggcgatgc
ctgccggattgctggaaagcgatctgtttggtcatgagcgtggggcttttaccggtgccagcgcccagcgtatcggtcg
ttttgaactggcggataaaagctccctgttcctcgacgaagtgggcgatatgccactggagttacagccgaagttgctg
cgtgtattgcaggaacaggagtttgaacgtctcggcagcaacaaaatcattcagacggacgtgcgtctaatcgccgcga
ctaaccgcgatctgaaaaaaatggtcgccgaccgtgagttccgtagcgatctctattaccgcctgaacgtattcccgat
tcacctgccgccactacgcgagcgtccggaagatattccgctgctggcgaaagcctttaccttcaaaattgcccgtcgt
ctggggcgcaatatcgacagcattcctgccgagacgctgcgcaccttgagcaacatggagtggccgggtaacgtacgcg
aactggaaaacgtcattgagcgcgcggtattgctaacacgcggtaacgtgctgcaactgtcattgccagatattgtttt
accggaacctgaaacgccgcctgccgcagcggttgtcgccctggagggcgaagatgaatatcagttgattgtgcgcgtg
ctgaaagaaaccaacggcgtggttgccgggcctaaaggcgctgcgcaacgtctggggctgaaacgcacgaccctgctgt
cacggatgaagcggctgggaattgataaatcggcattgatttaactgcaaattgccggacagatctgcctgtccggcat
actattctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaag
ccagattattaatccggcttttttattattt