LOCUS       BBa_K778008             2306 bp    DNA     linear       22-Nov-2024
DEFINITION  FhlA-E363G generator (pLac-RBS-fhlA363-d.term)
ACCESSION   BBa_K778008
VERSION     BBa_K778008.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     misc_feature    83..83
                     /label=start
     misc_feature    1170..1170
                     /label=E363G mutation
     misc_feature    2159..2161
                     /label=stop
     misc_feature    1988..1988
                     /label=A to G (different from E. coli K-12 MG1655)
     CDS             83..2161
                     /label=FhlA-E363G
     CDS             83..2203
                     /label=BBa_K778002
     stem_loop       2219..2238
                     /label=T7 TE
     misc_feature    2245..2245
                     /label=stop
     polyA_site      2239..2252
                     /label=polya
     misc_feature    2212..2252
                     /label=BBa_B0012
     terminator      2212..2252
                     /label=BBa_B0012
     stem_loop       2273..2295
                     /label=stem_loop
     misc_feature    2261..2306
                     /label=BBa_B0011
     terminator      2261..2306
                     /label=BBa_B0011
     terminator      2212..2306
                     /label=BBa_B0014
     misc_feature    64..2306
                     /label=BBa_K778005
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgtcata tacaccgatg agtgatctcg gacaacaagg
      121 gttgttcgac atcactcgga cactattgca gcagcccgat ctggcctcgc tgtgtgaggc
      181 tctttcgcaa ctggtaaagc gttctgcgct cgccgacaac gcggctattg tgttgtggca
      241 agcgcagact caacgtgcgt cttattacgc gtcgcgtgaa aaagacaccc ccattaaata
      301 tgaagacgaa actgttctgg cacacggtcc ggtacgcagc attttgtcgc gccctgatac
      361 gctgcattgc agttacgaag aattttgtga aacctggccg cagctggacg caggtgggct
      421 atacccaaaa tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg
      481 tggctgtgaa tttattcgtt atgacgatcg cccctggagc gaaaaagagt tcaatcgtct
      541 gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgttaa
      601 caatgtcgac tatgagttgt tatgccggga acgcgataac ttccgcatcc tggtcgccat
      661 caccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga
      721 aatccattac tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa
      781 caaactcaac atctactcca ctcactatct tgataaacag catcccgccc acgaacagag
      841 cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct
      901 gatcaatctc cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacacctg
      961 gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg
     1021 cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttact
     1081 gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat
     1141 ccatcgtctg aaagaacggc tggttgatgg aaacctcgcc ctgaccgagc agctcaacaa
     1201 tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa
     1261 acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac
     1321 gggtaaagag ctgattgccc gtgcgatcca taatctcagt gggcgtaata atcgccgcat
     1381 ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca
     1441 tgagcgtggg gcttttaccg gtgccagcgc ccagcgtatc ggtcgttttg aactggcgga
     1501 taaaagctcc ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt
     1561 gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac
     1621 ggacgtgcgt ctaatcgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgtga
     1681 gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg
     1741 cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg
     1801 tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga
     1861 gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg
     1921 cggtaacgtg ctgcaactgt cattgccaga tattgtttta ccggaacctg aaacgccgcc
     1981 tgccgcagcg gttgtcgccc tggagggcga agatgaatat cagttgattg tgcgcgtgct
     2041 gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa
     2101 acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta
     2161 actgcaaatt gccggacaga tctgcctgtc cggcatacta ttctactaga gtcacactgg
     2221 ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga
     2281 ttattaatcc ggctttttta ttattt
//