##gff-version 3
##sequence-region BBa_K778008 1 2306
BBa_K778008	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K778008	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K778008	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K778008	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K778008	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K778008	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K778008	.	engineered_region	64	2306	.	+	0	ID=BBa_K778005;Name=BBa_K778005
BBa_K778008	.	CDS	83	2203	.	+	0	ID=BBa_K778002;Name=BBa_K778002;Parent=BBa_K778005
BBa_K778008	.	sequence_alteration	1170	1170	.	+	0	ID=annotation2194869;Name=E363G mutation;Parent=BBa_K778002
BBa_K778008	.	stop_codon	2159	2161	.	+	0	ID=annotation2194870;Name=stop;Parent=BBa_K778002
BBa_K778008	.	start_codon	83	83	.	+	0	ID=annotation2194868;Name=start;Parent=BBa_K778002
BBa_K778008	.	CDS	83	2161	.	+	0	ID=annotation2201467;Name=FhlA-E363G;Parent=BBa_K778002
BBa_K778008	.	sequence_alteration	1988	1988	.	+	0	ID=annotation2195764;Name=A to G (different from E. coli K-12 MG1655);Parent=BBa_K778002
BBa_K778008	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K778005
BBa_K778008	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K778008	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K778008	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K778008	.	terminator	2212	2306	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K778005
BBa_K778008	.	terminator	2212	2252	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K778008	.	stop_codon	2245	2245	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K778008	.	polyA_site	2239	2252	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K778008	.	stem_loop	2219	2238	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K778008	.	engineered_region	2212	2252	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K778008	.	terminator	2261	2306	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K778008	.	engineered_region	2261	2306	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K778008	.	stem_loop	2273	2295	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_K778008
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgtcatatacaccgatgagtgatctcggacaacaagggttgttcgacatcactcggacactattgcagcagcccg
atctggcctcgctgtgtgaggctctttcgcaactggtaaagcgttctgcgctcgccgacaacgcggctattgtgttgtg
gcaagcgcagactcaacgtgcgtcttattacgcgtcgcgtgaaaaagacacccccattaaatatgaagacgaaactgtt
ctggcacacggtccggtacgcagcattttgtcgcgccctgatacgctgcattgcagttacgaagaattttgtgaaacct
ggccgcagctggacgcaggtgggctatacccaaaatttggtcactattgcctgatgccactggcggcggaagggcatat
ttttggtggctgtgaatttattcgttatgacgatcgcccctggagcgaaaaagagttcaatcgtctgcaaacatttacg
cagatcgtttctgtcgtcaccgaacaaatccagagccgcgtcgttaacaatgtcgactatgagttgttatgccgggaac
gcgataacttccgcatcctggtcgccatcaccaacgcggtgctttcccgcctggatatggacgaactggtcagcgaagt
cgccaaagaaatccattactatttcgacattgacgatatcagtatcgtcttacgcagccaccgtaaaaacaaactcaac
atctactccactcactatcttgataaacagcatcccgcccacgaacagagcgaagtcgatgaagccggaaccctcaccg
aacgcgtgttcaaaagtaaagagatgctgctgatcaatctccacgagcgggacgatttagccccctatgaacgcatgtt
gttcgacacctggggcaaccagattcaaaccttgtgcctgttaccgctgatgtctggcgacaccatgctgggcgtgctg
aaactggcgcaatgcgaagagaaagtgtttaccactaccaatctgaatttactgcgccagattgccgaacgtgtggcaa
tcgctgtcgataacgccctcgcctatcaggaaatccatcgtctgaaagaacggctggttgatggaaacctcgccctgac
cgagcagctcaacaatgttgatagtgaatttggcgagattattggccgcagcgaagccatgtacagcgtgcttaaacaa
gttgaaatggtggcgcaaagtgacagtaccgtgctgatcctcggtgaaactggcacgggtaaagagctgattgcccgtg
cgatccataatctcagtgggcgtaataatcgccgcatggtcaaaatgaactgcgcggcgatgcctgccggattgctgga
aagcgatctgtttggtcatgagcgtggggcttttaccggtgccagcgcccagcgtatcggtcgttttgaactggcggat
aaaagctccctgttcctcgacgaagtgggcgatatgccactggagttacagccgaagttgctgcgtgtattgcaggaac
aggagtttgaacgtctcggcagcaacaaaatcattcagacggacgtgcgtctaatcgccgcgactaaccgcgatctgaa
aaaaatggtcgccgaccgtgagttccgtagcgatctctattaccgcctgaacgtattcccgattcacctgccgccacta
cgcgagcgtccggaagatattccgctgctggcgaaagcctttaccttcaaaattgcccgtcgtctggggcgcaatatcg
acagcattcctgccgagacgctgcgcaccttgagcaacatggagtggccgggtaacgtacgcgaactggaaaacgtcat
tgagcgcgcggtattgctaacacgcggtaacgtgctgcaactgtcattgccagatattgttttaccggaacctgaaacg
ccgcctgccgcagcggttgtcgccctggagggcgaagatgaatatcagttgattgtgcgcgtgctgaaagaaaccaacg
gcgtggttgccgggcctaaaggcgctgcgcaacgtctggggctgaaacgcacgaccctgctgtcacggatgaagcggct
gggaattgataaatcggcattgatttaactgcaaattgccggacagatctgcctgtccggcatactattctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccg
gcttttttattattt