##gff-version 3
##sequence-region BBa_K778010 1 3394
BBa_K778010	.	engineered_region	1	1080	.	+	0	ID=BBa_K778009;Name=BBa_K778009
BBa_K778010	.	engineered_region	167	1080	.	+	0	ID=BBa_I763020;Name=BBa_I763020;Parent=BBa_K778009
BBa_K778010	.	ribosome_entry_site	167	178	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_K778010	.	conserved_region	171	174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K778010	.	engineered_region	185	943	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_K778010	.	stop_codon	938	943	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K778010	.	start_codon	185	187	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K778010	.	tag	899	937	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K778010	.	engineered_region	185	904	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K778010	.	terminator	1040	1080	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_K778010	.	stop_codon	1073	1073	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K778010	.	polyA_site	1067	1080	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K778010	.	stem_loop	1047	1066	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K778010	.	engineered_region	1040	1080	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K778010	.	terminator	952	1031	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_K778010	.	engineered_region	952	1031	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K778010	.	stem_loop	963	1006	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K778010	.	promoter	1	158	.	+	0	ID=BBa_K778000;Name=BBa_K778000;Parent=BBa_K778009
BBa_K778010	.	non_covalent_binding_site	42	76	.	+	0	ID=annotation2202206;Name=FhlA-binding site-2;Parent=BBa_K778000
BBa_K778010	.	start_codon	143	143	.	+	0	ID=annotation2192657;Name=Transcription Start Site;Parent=BBa_K778000
BBa_K778010	.	non_covalent_binding_site	29	61	.	+	0	ID=annotation2202204;Name=FhlA-binding site-1;Parent=BBa_K778000
BBa_K778010	.	engineered_region	1089	3394	.	+	0	ID=BBa_K778007;Name=BBa_K778007
BBa_K778010	.	engineered_region	1152	3394	.	+	0	ID=BBa_K778004;Name=BBa_K778004;Parent=BBa_K778007
BBa_K778010	.	CDS	1171	3291	.	+	0	ID=BBa_K778001;Name=BBa_K778001;Parent=BBa_K778004
BBa_K778010	.	point_mutation	3024	3024	.	+	0	ID=annotation2200692;Name=Illegal Pst1_site deletion (1854bp);Parent=BBa_K778001
BBa_K778010	.	stop_codon	3247	3249	.	+	0	ID=annotation2200694;Name=stop;Parent=BBa_K778001
BBa_K778010	.	CDS	1171	3249	.	+	0	ID=annotation2201429;Name=FhlA protein;Parent=BBa_K778001
BBa_K778010	.	sequence_alteration	3076	3076	.	+	0	ID=annotation2200693;Name=A to G (1906bp) (different from E. coli K-12 MG1655);Parent=BBa_K778001
BBa_K778010	.	start_codon	1171	1171	.	+	0	ID=annotation2200691;Name=start;Parent=BBa_K778001
BBa_K778010	.	terminator	3300	3394	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K778004
BBa_K778010	.	terminator	3300	3340	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K778010	.	stop_codon	3333	3333	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K778010	.	polyA_site	3327	3340	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K778010	.	stem_loop	3307	3326	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K778010	.	engineered_region	3300	3340	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K778010	.	terminator	3349	3394	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K778010	.	engineered_region	3349	3394	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K778010	.	stem_loop	3361	3383	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K778010	.	ribosome_entry_site	1152	1164	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K778004
BBa_K778010	.	ribosome_entry_site	1152	1164	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K778010	.	engineered_region	1152	1164	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K778010	.	ribosome_entry_site	1158	1161	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K778010	.	promoter	1089	1142	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K778007
BBa_K778010	.	promoter	1131	1136	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K778010	.	engineered_region	1089	1142	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K778010	.	operator	1091	1107	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K778010	.	promoter	1108	1113	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K778010	.	operator	1114	1130	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
>BBa_K778010
ccagtgttgattatccctgcggtgaataatgtcgatgatgtcgaaatgacacgtcgacacggcgacgaaattcatcttt
agcttaaaaatctctttaataacaataaattaaaagttggcacaaaaaatgcttaaagctggcatctctgttaaacggg
tactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatt
agatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctt
aaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgct
ttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaac
tatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatc
gagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatg
tatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgt
tcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcc
acacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
ttacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataaca
attgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgtcatatacaccg
atgagtgatctcggacaacaagggttgttcgacatcactcggacactattgcagcagcccgatctggcctcgctgtgtg
aggctctttcgcaactggtaaagcgttctgcgctcgccgacaacgcggctattgtgttgtggcaagcgcagactcaacg
tgcgtcttattacgcgtcgcgtgaaaaagacacccccattaaatatgaagacgaaactgttctggcacacggtccggta
cgcagcattttgtcgcgccctgatacgctgcattgcagttacgaagaattttgtgaaacctggccgcagctggacgcag
gtgggctatacccaaaatttggtcactattgcctgatgccactggcggcggaagggcatatttttggtggctgtgaatt
tattcgttatgacgatcgcccctggagcgaaaaagagttcaatcgtctgcaaacatttacgcagatcgtttctgtcgtc
accgaacaaatccagagccgcgtcgttaacaatgtcgactatgagttgttatgccgggaacgcgataacttccgcatcc
tggtcgccatcaccaacgcggtgctttcccgcctggatatggacgaactggtcagcgaagtcgccaaagaaatccatta
ctatttcgacattgacgatatcagtatcgtcttacgcagccaccgtaaaaacaaactcaacatctactccactcactat
cttgataaacagcatcccgcccacgaacagagcgaagtcgatgaagccggaaccctcaccgaacgcgtgttcaaaagta
aagagatgctgctgatcaatctccacgagcgggacgatttagccccctatgaacgcatgttgttcgacacctggggcaa
ccagattcaaaccttgtgcctgttaccgctgatgtctggcgacaccatgctgggcgtgctgaaactggcgcaatgcgaa
gagaaagtgtttaccactaccaatctgaatttactgcgccagattgccgaacgtgtggcaatcgctgtcgataacgccc
tcgcctatcaggaaatccatcgtctgaaagaacggctggttgatgaaaacctcgccctgaccgagcagctcaacaatgt
tgatagtgaatttggcgagattattggccgcagcgaagccatgtacagcgtgcttaaacaagttgaaatggtggcgcaa
agtgacagtaccgtgctgatcctcggtgaaactggcacgggtaaagagctgattgcccgtgcgatccataatctcagtg
ggcgtaataatcgccgcatggtcaaaatgaactgcgcggcgatgcctgccggattgctggaaagcgatctgtttggtca
tgagcgtggggcttttaccggtgccagcgcccagcgtatcggtcgttttgaactggcggataaaagctccctgttcctc
gacgaagtgggcgatatgccactggagttacagccgaagttgctgcgtgtattgcaggaacaggagtttgaacgtctcg
gcagcaacaaaatcattcagacggacgtgcgtctaatcgccgcgactaaccgcgatctgaaaaaaatggtcgccgaccg
tgagttccgtagcgatctctattaccgcctgaacgtattcccgattcacctgccgccactacgcgagcgtccggaagat
attccgctgctggcgaaagcctttaccttcaaaattgcccgtcgtctggggcgcaatatcgacagcattcctgccgaga
cgctgcgcaccttgagcaacatggagtggccgggtaacgtacgcgaactggaaaacgtcattgagcgcgcggtattgct
aacacgcggtaacgtgctgcaactgtcattgccagatattgttttaccggaacctgaaacgccgcctgccgcagcggtt
gtcgccctggagggcgaagatgaatatcagttgattgtgcgcgtgctgaaagaaaccaacggcgtggttgccgggccta
aaggcgctgcgcaacgtctggggctgaaacgcacgaccctgctgtcacggatgaagcggctgggaattgataaatcggc
attgatttaactgcaaattgccggacagatctgcctgtccggcatactattctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt