LOCUS BBa_K778011 3394 bp DNA linear 16-Jun-2024 DEFINITION hycAp-GFPgenerator-plac-FhlA363generator ACCESSION BBa_K778011 VERSION BBa_K778011.1 FEATURES Location/Qualifiers misc_feature 143..143 /label=Transcription Start Site misc_feature 29..61 /label=FhlA-binding site-1 misc_feature 42..76 /label=FhlA-binding site-2 promoter 1..158 /label=BBa_K778000 misc_feature 171..174 /label=conserved RBS 167..178 /label=BBa_B0034 misc_feature 185..904 /label=BBa_E0040 misc_feature 899..937 /label=LVA misc_feature 938..943 /label=Double Stop misc_feature 185..187 /label=Start misc_feature 185..943 /label=BBa_J04031 stem_loop 963..1006 /label=stem_loop misc_feature 952..1031 /label=BBa_B0010 terminator 952..1031 /label=BBa_B0010 stem_loop 1047..1066 /label=T7 TE misc_feature 1073..1073 /label=stop polyA_site 1067..1080 /label=polya misc_feature 1040..1080 /label=BBa_B0012 terminator 1040..1080 /label=BBa_B0012 misc_feature 167..1080 /label=BBa_I763020 misc_feature 1..1080 /label=BBa_K778009 misc_feature 1091..1107 /label=lac O1 promoter 1108..1113 /label=-35 misc_feature 1114..1130 /label=lac O1 promoter 1131..1136 /label=-10 misc_feature 1089..1142 /label=BBa_R0011 promoter 1089..1142 /label=BBa_R0011 RBS 1152..1164 /label=RBS-3\Weak RBS 1158..1161 /label=RBS misc_feature 1152..1164 /label=BBa_B0032 RBS 1152..1164 /label=BBa_B0032 misc_feature 1171..1171 /label=start misc_feature 2258..2258 /label=E363G mutation misc_feature 3247..3249 /label=stop misc_feature 3076..3076 /label=A to G (different from E. coli K-12 MG1655) CDS 1171..3249 /label=FhlA-E363G CDS 1171..3291 /label=BBa_K778002 stem_loop 3307..3326 /label=T7 TE misc_feature 3333..3333 /label=stop polyA_site 3327..3340 /label=polya misc_feature 3300..3340 /label=BBa_B0012 terminator 3300..3340 /label=BBa_B0012 stem_loop 3361..3383 /label=stem_loop misc_feature 3349..3394 /label=BBa_B0011 terminator 3349..3394 /label=BBa_B0011 terminator 3300..3394 /label=BBa_B0014 misc_feature 1152..3394 /label=BBa_K778005 misc_feature 1089..3394 /label=BBa_K778008 ORIGIN 1 ccagtgttga ttatccctgc ggtgaataat gtcgatgatg tcgaaatgac acgtcgacac 61 ggcgacgaaa ttcatcttta gcttaaaaat ctctttaata acaataaatt aaaagttggc 121 acaaaaaatg cttaaagctg gcatctctgt taaacgggta ctagagaaag aggagaaata 181 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga 241 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata 301 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac 361 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa 421 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt 481 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct 541 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca 601 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa 661 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc 721 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca 781 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt 841 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaaag 901 gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga gccaggcatc 961 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1021 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1081 tactagagaa ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc 1141 acatactaga gtcacacagg aaagtactag atgtcatata caccgatgag tgatctcgga 1201 caacaagggt tgttcgacat cactcggaca ctattgcagc agcccgatct ggcctcgctg 1261 tgtgaggctc tttcgcaact ggtaaagcgt tctgcgctcg ccgacaacgc ggctattgtg 1321 ttgtggcaag cgcagactca acgtgcgtct tattacgcgt cgcgtgaaaa agacaccccc 1381 attaaatatg aagacgaaac tgttctggca cacggtccgg tacgcagcat tttgtcgcgc 1441 cctgatacgc tgcattgcag ttacgaagaa ttttgtgaaa cctggccgca gctggacgca 1501 ggtgggctat acccaaaatt tggtcactat tgcctgatgc cactggcggc ggaagggcat 1561 atttttggtg gctgtgaatt tattcgttat gacgatcgcc cctggagcga aaaagagttc 1621 aatcgtctgc aaacatttac gcagatcgtt tctgtcgtca ccgaacaaat ccagagccgc 1681 gtcgttaaca atgtcgacta tgagttgtta tgccgggaac gcgataactt ccgcatcctg 1741 gtcgccatca ccaacgcggt gctttcccgc ctggatatgg acgaactggt cagcgaagtc 1801 gccaaagaaa tccattacta tttcgacatt gacgatatca gtatcgtctt acgcagccac 1861 cgtaaaaaca aactcaacat ctactccact cactatcttg ataaacagca tcccgcccac 1921 gaacagagcg aagtcgatga agccggaacc ctcaccgaac gcgtgttcaa aagtaaagag 1981 atgctgctga tcaatctcca cgagcgggac gatttagccc cctatgaacg catgttgttc 2041 gacacctggg gcaaccagat tcaaaccttg tgcctgttac cgctgatgtc tggcgacacc 2101 atgctgggcg tgctgaaact ggcgcaatgc gaagagaaag tgtttaccac taccaatctg 2161 aatttactgc gccagattgc cgaacgtgtg gcaatcgctg tcgataacgc cctcgcctat 2221 caggaaatcc atcgtctgaa agaacggctg gttgatggaa acctcgccct gaccgagcag 2281 ctcaacaatg ttgatagtga atttggcgag attattggcc gcagcgaagc catgtacagc 2341 gtgcttaaac aagttgaaat ggtggcgcaa agtgacagta ccgtgctgat cctcggtgaa 2401 actggcacgg gtaaagagct gattgcccgt gcgatccata atctcagtgg gcgtaataat 2461 cgccgcatgg tcaaaatgaa ctgcgcggcg atgcctgccg gattgctgga aagcgatctg 2521 tttggtcatg agcgtggggc ttttaccggt gccagcgccc agcgtatcgg tcgttttgaa 2581 ctggcggata aaagctccct gttcctcgac gaagtgggcg atatgccact ggagttacag 2641 ccgaagttgc tgcgtgtatt gcaggaacag gagtttgaac gtctcggcag caacaaaatc 2701 attcagacgg acgtgcgtct aatcgccgcg actaaccgcg atctgaaaaa aatggtcgcc 2761 gaccgtgagt tccgtagcga tctctattac cgcctgaacg tattcccgat tcacctgccg 2821 ccactacgcg agcgtccgga agatattccg ctgctggcga aagcctttac cttcaaaatt 2881 gcccgtcgtc tggggcgcaa tatcgacagc attcctgccg agacgctgcg caccttgagc 2941 aacatggagt ggccgggtaa cgtacgcgaa ctggaaaacg tcattgagcg cgcggtattg 3001 ctaacacgcg gtaacgtgct gcaactgtca ttgccagata ttgttttacc ggaacctgaa 3061 acgccgcctg ccgcagcggt tgtcgccctg gagggcgaag atgaatatca gttgattgtg 3121 cgcgtgctga aagaaaccaa cggcgtggtt gccgggccta aaggcgctgc gcaacgtctg 3181 gggctgaaac gcacgaccct gctgtcacgg atgaagcggc tgggaattga taaatcggca 3241 ttgatttaac tgcaaattgc cggacagatc tgcctgtccg gcatactatt ctactagagt 3301 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa 3361 aagccagatt attaatccgg cttttttatt attt //