BBa_K778011
type: ComponentDefinition
persistent identity: BBa K778011
display id: BBa_K778011
version: 1
was generated by: iGEM to SBOL conversion
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:17Z
mutable provenance: biobrick parts
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 363
star: false
creator: Yuka Yamazaki
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K778008
component: BBa_K778009
sequence annotation: BBa_K778009
sequence annotation: BBa_K778008
sequence: https://synbiohub.org/public/igem/BBa_K778011_sequence/1

BBa_K778008
type: Component
persistent identity: component2230988
display id: component2230988
version: 1
top level: BBa_K778011
definition: BBa_K778008
access: public

BBa_K778009
type: Component
persistent identity: component2230966
display id: component2230966
version: 1
top level: BBa_K778011
definition: hycAp GFP
access: public

BBa_K778009
type: SequenceAnnotation
persistent identity: annotation2230966
display id: annotation2230966
version: 1
top level: BBa_K778011
location: https://synbiohub.org/public/igem/BBa_K778011/annotation2230966/range2230966/1
component: BBa_K778009

https://synbiohub.org/public/igem/BBa_K778011/annotation2230966/range2230966/1
type: Range
persistent identity: range2230966
display id: range2230966
version: 1
top level: BBa_K778011
direction: direction/reverse
start: 1
end: 1080
orientation: inline

BBa_K778008
type: SequenceAnnotation
persistent identity: annotation2230988
display id: annotation2230988
version: 1
top level: BBa_K778011
location: https://synbiohub.org/public/igem/BBa_K778011/annotation2230988/range2230988/1
component: BBa_K778008

https://synbiohub.org/public/igem/BBa_K778011/annotation2230988/range2230988/1
type: Range
persistent identity: range2230988
display id: range2230988
version: 1
top level: BBa_K778011
direction: direction/reverse
start: 1089
end: 3394
orientation: inline

BBa_K778008
type: ComponentDefinition
persistent identity: BBa K778008
display id: BBa_K778008
version: 1
was generated by: iGEM to SBOL conversion
description: FhlA-E363G generator (pLac-RBS-fhlA363-d.term)
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:17Z
mutable provenance: biobrick parts
owned by: james
owned by: myers
mutable description: plac-rbs-fhlA363-d.term
discontinued: false
dominant: false
experience: experience/Works
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: 363
star: false
creator: Yuka Yamazaki
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K778005
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_K778005
sequence: https://synbiohub.org/public/igem/BBa_K778008_sequence/1

BBa_K778005
type: Component
persistent identity: component2223247
display id: component2223247
version: 1
top level: BBa_K778008
definition: BBa_K778005
access: public

BBa_R0011
type: Component
persistent identity: component2223227
display id: component2223227
version: 1
top level: BBa_K778008
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2223227
display id: annotation2223227
version: 1
top level: BBa_K778008
location: https://synbiohub.org/public/igem/BBa_K778008/annotation2223227/range2223227/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K778008/annotation2223227/range2223227/1
type: Range
persistent identity: range2223227
display id: range2223227
version: 1
top level: BBa_K778008
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K778005
type: SequenceAnnotation
persistent identity: annotation2223247
display id: annotation2223247
version: 1
top level: BBa_K778008
location: https://synbiohub.org/public/igem/BBa_K778008/annotation2223247/range2223247/1
component: BBa_K778005

https://synbiohub.org/public/igem/BBa_K778008/annotation2223247/range2223247/1
type: Range
persistent identity: range2223247
display id: range2223247
version: 1
top level: BBa_K778008
direction: direction/reverse
start: 64
end: 2306
orientation: inline

hycAp GFP
type: ComponentDefinition
persistent identity: BBa K778009
display id: BBa_K778009
version: 1
was generated by: iGEM to SBOL conversion
description: hycAp-GFPgenerater
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:17Z
mutable provenance: biobrick part
owned by: james
owned by: myers
mutable description: hycAp-GFPgenerater
discontinued: false
dominant: false
experience: experience/None
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no consideration
star: false
creator: Yuka Yamazaki
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I763020
component: BBa_K778000
sequence annotation: BBa_K778000
sequence annotation: BBa_I763020
sequence: https://synbiohub.org/public/igem/BBa_K778009_sequence/1

BBa_I763020
type: Component
persistent identity: component2201945
display id: component2201945
version: 1
top level: hycAp GFP
definition: RBS-GFP-Te
access: public

BBa_K778000
type: Component
persistent identity: component2201931
display id: component2201931
version: 1
top level: hycAp GFP
definition: hycAp
access: public

BBa_K778000
type: SequenceAnnotation
persistent identity: annotation2201931
display id: annotation2201931
version: 1
top level: hycAp GFP
location: https://synbiohub.org/public/igem/BBa_K778009/annotation2201931/range2201931/1
component: BBa_K778000

https://synbiohub.org/public/igem/BBa_K778009/annotation2201931/range2201931/1
type: Range
persistent identity: range2201931
display id: range2201931
version: 1
top level: hycAp GFP
direction: direction/reverse
start: 1
end: 158
orientation: inline

BBa_I763020
type: SequenceAnnotation
persistent identity: annotation2201945
display id: annotation2201945
version: 1
top level: hycAp GFP
location: https://synbiohub.org/public/igem/BBa_K778009/annotation2201945/range2201945/1
component: BBa_I763020

https://synbiohub.org/public/igem/BBa_K778009/annotation2201945/range2201945/1
type: Range
persistent identity: range2201945
display id: range2201945
version: 1
top level: hycAp GFP
direction: direction/reverse
start: 167
end: 1080
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

RBS-GFP-Te
type: ComponentDefinition
persistent identity: BBa I763020
display id: BBa_I763020
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-GFP (+LVA Tag)-Terminators
created: 2007-08-26T11:00:00Z
modified: 2015-08-31T04:08:08Z
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Intermediate part composed by RBS (B0034), GFP with LVA tag and terminators.
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Intermediate
star: false
creator: Alice Pasini
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J04031
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J04031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I763020_sequence/1

BBa_J04031
type: Component
persistent identity: component1942525
display id: component1942525
version: 1
top level: RBS-GFP-Te
definition: BBa_J04031
access: public

BBa_B0034
type: Component
persistent identity: component1942520
display id: component1942520
version: 1
top level: RBS-GFP-Te
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1942528
display id: component1942528
version: 1
top level: RBS-GFP-Te
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1942526
display id: component1942526
version: 1
top level: RBS-GFP-Te
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1942520
display id: annotation1942520
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942520/range1942520/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I763020/annotation1942520/range1942520/1
type: Range
persistent identity: range1942520
display id: range1942520
version: 1
top level: RBS-GFP-Te
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation1942525
display id: annotation1942525
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942525/range1942525/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_I763020/annotation1942525/range1942525/1
type: Range
persistent identity: range1942525
display id: range1942525
version: 1
top level: RBS-GFP-Te
direction: direction/reverse
start: 19
end: 777
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1942526
display id: annotation1942526
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942526/range1942526/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I763020/annotation1942526/range1942526/1
type: Range
persistent identity: range1942526
display id: range1942526
version: 1
top level: RBS-GFP-Te
direction: direction/forward
start: 786
end: 865
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1942528
display id: annotation1942528
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942528/range1942528/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I763020/annotation1942528/range1942528/1
type: Range
persistent identity: range1942528
display id: range1942528
version: 1
top level: RBS-GFP-Te
direction: direction/forward
start: 874
end: 914
orientation: inline

hycAp
type: ComponentDefinition
persistent identity: BBa K778000
display id: BBa_K778000
version: 1
was generated by: iGEM to SBOL conversion
description: hycAp: Promoter of hyc operon activated by FhlA
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
mutable provenance: Escherichia coli (JM109)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: hycAp is promoter activated by FhlA (transcriptional activater)
discontinued: false
dominant: false
experience: experience/None
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: mutation?
star: false
creator: Yuka Yamazaki
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: FhlA-binding site-1
sequence annotation: FhlA-binding site-2
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_K778000_sequence/1

FhlA-binding site-1
type: SequenceAnnotation
persistent identity: annotation2202204
display id: annotation2202204
version: 1
top level: hycAp
location: https://synbiohub.org/public/igem/BBa_K778000/annotation2202204/range2202204/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K778000/annotation2202204/range2202204/1
type: Range
persistent identity: range2202204
display id: range2202204
version: 1
top level: hycAp
direction: direction/forward
start: 29
end: 61
orientation: inline

FhlA-binding site-2
type: SequenceAnnotation
persistent identity: annotation2202206
display id: annotation2202206
version: 1
top level: hycAp
location: https://synbiohub.org/public/igem/BBa_K778000/annotation2202206/range2202206/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K778000/annotation2202206/range2202206/1
type: Range
persistent identity: range2202206
display id: range2202206
version: 1
top level: hycAp
direction: direction/forward
start: 42
end: 76
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation2192657
display id: annotation2192657
version: 1
top level: hycAp
location: https://synbiohub.org/public/igem/BBa_K778000/annotation2192657/range2192657/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K778000/annotation2192657/range2192657/1
type: Range
persistent identity: range2192657
display id: range2192657
version: 1
top level: hycAp
direction: direction/forward
start: 143
end: 143
orientation: inline

BBa_K778005
type: ComponentDefinition
persistent identity: BBa K778005
display id: BBa_K778005
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-fhlA363-d.term
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:17Z
mutable provenance: biobrick parts
owned by: james
owned by: myers
mutable description: rbs-fhlA363-d.term
discontinued: false
dominant: false
experience: experience/Works
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: 363
star: false
creator: Yuka Yamazaki
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0014
component: BBa_K778002
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K778002
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K778005_sequence/1

BBa_B0014
type: Component
persistent identity: component2220976
display id: component2220976
version: 1
top level: BBa_K778005
definition: BBa_B0014
access: public

BBa_K778002
type: Component
persistent identity: component2220969
display id: component2220969
version: 1
top level: BBa_K778005
definition: fhlA-E363
access: public

BBa_B0032
type: Component
persistent identity: component2220963
display id: component2220963
version: 1
top level: BBa_K778005
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2220963
display id: annotation2220963
version: 1
top level: BBa_K778005
location: https://synbiohub.org/public/igem/BBa_K778005/annotation2220963/range2220963/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K778005/annotation2220963/range2220963/1
type: Range
persistent identity: range2220963
display id: range2220963
version: 1
top level: BBa_K778005
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K778002
type: SequenceAnnotation
persistent identity: annotation2220969
display id: annotation2220969
version: 1
top level: BBa_K778005
location: https://synbiohub.org/public/igem/BBa_K778005/annotation2220969/range2220969/1
component: BBa_K778002

https://synbiohub.org/public/igem/BBa_K778005/annotation2220969/range2220969/1
type: Range
persistent identity: range2220969
display id: range2220969
version: 1
top level: BBa_K778005
direction: direction/reverse
start: 20
end: 2140
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2220976
display id: annotation2220976
version: 1
top level: BBa_K778005
location: https://synbiohub.org/public/igem/BBa_K778005/annotation2220976/range2220976/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K778005/annotation2220976/range2220976/1
type: Range
persistent identity: range2220976
display id: range2220976
version: 1
top level: BBa_K778005
direction: direction/reverse
start: 2149
end: 2243
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_J04031
type: ComponentDefinition
persistent identity: BBa J04031
display id: BBa_J04031
version: 1
was generated by: iGEM to SBOL conversion
description: GFP w/LVA tag
created: 2005-06-07T11:00:00Z
modified: 2015-08-31T04:08:13Z
mutable provenance: Davidson Synth Aces
owned by: james
owned by: myers
mutable description: BBa_E0040 with an LVA tag added via PCR. BBa_E0040 is GFP(mut3b).
discontinued: false
dominant: true
experience: experience/None
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0040
sequence annotation: Start
sequence annotation: LVA
sequence annotation: Double Stop
sequence: https://synbiohub.org/public/igem/BBa_J04031_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1507202
display id: annotation1507202
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507202/range1507202/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J04031/annotation1507202/range1507202/1
type: Range
persistent identity: range1507202
display id: range1507202
version: 1
top level: BBa_J04031
direction: direction/forward
start: 1
end: 720
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation1507246
display id: annotation1507246
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507246/range1507246/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J04031/annotation1507246/range1507246/1
type: Range
persistent identity: range1507246
display id: range1507246
version: 1
top level: BBa_J04031
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1507235
display id: annotation1507235
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507235/range1507235/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J04031/annotation1507235/range1507235/1
type: Range
persistent identity: range1507235
display id: range1507235
version: 1
top level: BBa_J04031
direction: direction/forward
start: 715
end: 753
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation1507242
display id: annotation1507242
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507242/range1507242/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J04031/annotation1507242/range1507242/1
type: Range
persistent identity: range1507242
display id: range1507242
version: 1
top level: BBa_J04031
direction: direction/forward
start: 754
end: 759
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

fhlA-E363
type: ComponentDefinition
persistent identity: BBa K778002
display id: BBa_K778002
version: 1
was generated by: iGEM to SBOL conversion
description: fhlA-E363G (engineered trascriptional activator fhlA with a mutation)
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:17Z
mutable provenance: BBa_K778001
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: fhlA363
discontinued: false
dominant: false
experience: experience/Works
group u list: _1032_
m user id: 0
owner id: 13134
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: E363G
star: false
creator: Yuka Yamazaki
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start
sequence annotation: FhlA-E363G
sequence annotation: E363G mutation
sequence annotation: A to G (different from E. coli K-12 MG1655)
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K778002_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2194868
display id: annotation2194868
version: 1
top level: fhlA-E363
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194868/range2194868/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K778002/annotation2194868/range2194868/1
type: Range
persistent identity: range2194868
display id: range2194868
version: 1
top level: fhlA-E363
direction: direction/forward
start: 1
end: 1
orientation: inline

FhlA-E363G
type: SequenceAnnotation
persistent identity: annotation2201467
display id: annotation2201467
version: 1
top level: fhlA-E363
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2201467/range2201467/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K778002/annotation2201467/range2201467/1
type: Range
persistent identity: range2201467
display id: range2201467
version: 1
top level: fhlA-E363
direction: direction/forward
start: 1
end: 2079
orientation: inline

E363G mutation
type: SequenceAnnotation
persistent identity: annotation2194869
display id: annotation2194869
version: 1
top level: fhlA-E363
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194869/range2194869/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K778002/annotation2194869/range2194869/1
type: Range
persistent identity: range2194869
display id: range2194869
version: 1
top level: fhlA-E363
direction: direction/forward
start: 1088
end: 1088
orientation: inline

A to G (different from E. coli K-12 MG1655)
type: SequenceAnnotation
persistent identity: annotation2195764
display id: annotation2195764
version: 1
top level: fhlA-E363
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2195764/range2195764/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K778002/annotation2195764/range2195764/1
type: Range
persistent identity: range2195764
display id: range2195764
version: 1
top level: fhlA-E363
direction: direction/forward
start: 1906
end: 1906
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2194870
display id: annotation2194870
version: 1
top level: fhlA-E363
location: https://synbiohub.org/public/igem/BBa_K778002/annotation2194870/range2194870/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K778002/annotation2194870/range2194870/1
type: Range
persistent identity: range2194870
display id: range2194870
version: 1
top level: fhlA-E363
direction: direction/forward
start: 2077
end: 2079
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

https://synbiohub.org/public/igem/BBa_K778011_sequence/1
type: Sequence
persistent identity: BBa K778011 sequence
display id: BBa_K778011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ccagtgttgattatccctgcggtgaataatgtcgatgatgtcgaaatgacacgtcgacacggcgacgaaattcatctttagcttaaaaatctctttaataacaataaattaaaagttggcacaaaaaatgcttaaagctggcatctctgttaaacgggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgtcatatacaccgatgagtgatctcggacaacaagggttgttcgacatcactcggacactattgcagcagcccgatctggcctcgctgtgtgaggctctttcgcaactggtaaagcgttctgcgctcgccgacaacgcggctattgtgttgtggcaagcgcagactcaacgtgcgtcttattacgcgtcgcgtgaaaaagacacccccattaaatatgaagacgaaactgttctggcacacggtccggtacgcagcattttgtcgcgccctgatacgctgcattgcagttacgaagaattttgtgaaacctggccgcagctggacgcaggtgggctatacccaaaatttggtcactattgcctgatgccactggcggcggaagggcatatttttggtggctgtgaatttattcgttatgacgatcgcccctggagcgaaaaagagttcaatcgtctgcaaacatttacgcagatcgtttctgtcgtcaccgaacaaatccagagccgcgtcgttaacaatgtcgactatgagttgttatgccgggaacgcgataacttccgcatcctggtcgccatcaccaacgcggtgctttcccgcctggatatggacgaactggtcagcgaagtcgccaaagaaatccattactatttcgacattgacgatatcagtatcgtcttacgcagccaccgtaaaaacaaactcaacatctactccactcactatcttgataaacagcatcccgcccacgaacagagcgaagtcgatgaagccggaaccctcaccgaacgcgtgttcaaaagtaaagagatgctgctgatcaatctcca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K778008_sequence/1
type: Sequence
persistent identity: BBa K778008 sequence
display id: BBa_K778008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgtcatatacaccgatgagtgatctcggacaacaagggttgttcgacatcactcggacactattgcagcagcccgatctggcctcgctgtgtgaggctctttcgcaactggtaaagcgttctgcgctcgccgacaacgcggctattgtgttgtggcaagcgcagactcaacgtgcgtcttattacgcgtcgcgtgaaaaagacacccccattaaatatgaagacgaaactgttctggcacacggtccggtacgcagcattttgtcgcgccctgatacgctgcattgcagttacgaagaattttgtgaaacctggccgcagctggacgcaggtgggctatacccaaaatttggtcactattgcctgatgccactggcggcggaagggcatatttttggtggctgtgaatttattcgttatgacgatcgcccctggagcgaaaaagagttcaatcgtctgcaaacatttacgcagatcgtttctgtcgtcaccgaacaaatccagagccgcgtcgttaacaatgtcgactatgagttgttatgccgggaacgcgataacttccgcatcctggtcgccatcaccaacgcggtgctttcccgcctggatatggacgaactggtcagcgaagtcgccaaagaaatccattactatttcgacattgacgatatcagtatcgtcttacgcagccaccgtaaaaacaaactcaacatctactccactcactatcttgataaacagcatcccgcccacgaacagagcgaagtcgatgaagccggaaccctcaccgaacgcgtgttcaaaagtaaagagatgctgctgatcaatctccacgagcgggacgatttagccccctatgaacgcatgttgttcgacacctggggcaaccagattcaaaccttgtgcctgttaccgctgatgtctggcgacaccatgctgggcgtgctgaaactggcgcaatgcgaagagaaagtgtttaccactaccaatctgaatttactgcgccagattgccgaacgtgtggcaatcgctgtcgataacgccctcgcctatcaggaaatccatcgtctgaaagaacggctggttgatggaaacctcgccctgaccgagcagctcaacaatgttgatagtgaatttggcgagattattggccgcagcgaagccatgtacagcgtgcttaaacaagttgaaatggtggcgcaaagtgacagtaccgtgctgatcctcggtgaaactggcacgggtaaagagctgattgcccgtgcgatccataatctcagtgggcgtaataatcgccgcatggtcaaaatgaactgcgcggcgatgcctgccggattgctggaaagcgatctgtttggtcatgagcgtggggcttttaccggtgccagcgcccagcgtatcggtcgttttgaactggcggataaaagctccctgttcctcgacgaagtgggcgatatgccactggagttacagccgaagttgctgcgtgtattgcaggaacaggagtttgaacgtctcggcagcaacaaaatcattcagacggacgtgcgtctaatcgccgcgactaaccgcgatctgaaaaaaatggtcgccgaccgtgagttccgtagcgatctctattaccgcctgaacgtattcccgattcacctgccgccactacgcgagcgtccggaagatattccgctgctggcgaaagcctttaccttcaaaattgcccgtcgtctggggcgcaatatcgacagcattcctgccgagacgctgcgcaccttgagcaacatggagtggccgggtaacgtacgcgaactggaaaacgtcattgagcgcgcggtattgctaacacgcggtaacgtgctgcaactgtcattgccagatattgttttaccggaacctgaaacgccgcctgccgcagcggttgtcgccc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K778009_sequence/1
type: Sequence
persistent identity: BBa K778009 sequence
display id: BBa_K778009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccagtgttgattatccctgcggtgaataatgtcgatgatgtcgaaatgacacgtcgacacggcgacgaaattcatctttagcttaaaaatctctttaataacaataaattaaaagttggcacaaaaaatgcttaaagctggcatctctgttaaacgggtactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I763020_sequence/1
type: Sequence
persistent identity: BBa I763020 sequence
display id: BBa_I763020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K778000_sequence/1
type: Sequence
persistent identity: BBa K778000 sequence
display id: BBa_K778000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccagtgttgattatccctgcggtgaataatgtcgatgatgtcgaaatgacacgtcgacacggcgacgaaattcatctttagcttaaaaatctctttaataacaataaattaaaagttggcacaaaaaatgcttaaagctggcatctctgttaaacggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K778005_sequence/1
type: Sequence
persistent identity: BBa K778005 sequence
display id: BBa_K778005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgtcatatacaccgatgagtgatctcggacaacaagggttgttcgacatcactcggacactattgcagcagcccgatctggcctcgctgtgtgaggctctttcgcaactggtaaagcgttctgcgctcgccgacaacgcggctattgtgttgtggcaagcgcagactcaacgtgcgtcttattacgcgtcgcgtgaaaaagacacccccattaaatatgaagacgaaactgttctggcacacggtccggtacgcagcattttgtcgcgccctgatacgctgcattgcagttacgaagaattttgtgaaacctggccgcagctggacgcaggtgggctatacccaaaatttggtcactattgcctgatgccactggcggcggaagggcatatttttggtggctgtgaatttattcgttatgacgatcgcccctggagcgaaaaagagttcaatcgtctgcaaacatttacgcagatcgtttctgtcgtcaccgaacaaatccagagccgcgtcgttaacaatgtcgactatgagttgttatgccgggaacgcgataacttccgcatcctggtcgccatcaccaacgcggtgctttcccgcctggatatggacgaactggtcagcgaagtcgccaaagaaatccattactatttcgacattgacgatatcagtatcgtcttacgcagccaccgtaaaaacaaactcaacatctactccactcactatcttgataaacagcatcccgcccacgaacagagcgaagtcgatgaagccggaaccctcaccgaacgcgtgttcaaaagtaaagagatgctgctgatcaatctccacgagcgggacgatttagccccctatgaacgcatgttgttcgacacctggggcaaccagattcaaaccttgtgcctgttaccgctgatgtctggcgacaccatgctgggcgtgctgaaactggcgcaatgcgaagagaaagtgtttaccactaccaatctgaatttactgcgccagattgccgaacgtgtggcaatcgctgtcgataacgccctcgcctatcaggaaatccatcgtctgaaagaacggctggttgatggaaacctcgccctgaccgagcagctcaacaatgttgatagtgaatttggcgagattattggccgcagcgaagccatgtacagcgtgcttaaacaagttgaaatggtggcgcaaagtgacagtaccgtgctgatcctcggtgaaactggcacgggtaaagagctgattgcccgtgcgatccataatctcagtgggcgtaataatcgccgcatggtcaaaatgaactgcgcggcgatgcctgccggattgctggaaagcgatctgtttggtcatgagcgtggggcttttaccggtgccagcgcccagcgtatcggtcgttttgaactggcggataaaagctccctgttcctcgacgaagtgggcgatatgccactggagttacagccgaagttgctgcgtgtattgcaggaacaggagtttgaacgtctcggcagcaacaaaatcattcagacggacgtgcgtctaatcgccgcgactaaccgcgatctgaaaaaaatggtcgccgaccgtgagttccgtagcgatctctattaccgcctgaacgtattcccgattcacctgccgccactacgcgagcgtccggaagatattccgctgctggcgaaagcctttaccttcaaaattgcccgtcgtctggggcgcaatatcgacagcattcctgccgagacgctgcgcaccttgagcaacatggagtggccgggtaacgtacgcgaactggaaaacgtcattgagcgcgcggtattgctaacacgcggtaacgtgctgcaactgtcattgccagatattgttttaccggaacctgaaacgccgcctgccgcagcggttgtcgccctggagggcgaagatgaatatcagttgattgtgcgcgtgctgaaagaaaccaacggcgtggttg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04031_sequence/1
type: Sequence
persistent identity: BBa J04031 sequence
display id: BBa_J04031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K778002_sequence/1
type: Sequence
persistent identity: BBa K778002 sequence
display id: BBa_K778002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcatatacaccgatgagtgatctcggacaacaagggttgttcgacatcactcggacactattgcagcagcccgatctggcctcgctgtgtgaggctctttcgcaactggtaaagcgttctgcgctcgccgacaacgcggctattgtgttgtggcaagcgcagactcaacgtgcgtcttattacgcgtcgcgtgaaaaagacacccccattaaatatgaagacgaaactgttctggcacacggtccggtacgcagcattttgtcgcgccctgatacgctgcattgcagttacgaagaattttgtgaaacctggccgcagctggacgcaggtgggctatacccaaaatttggtcactattgcctgatgccactggcggcggaagggcatatttttggtggctgtgaatttattcgttatgacgatcgcccctggagcgaaaaagagttcaatcgtctgcaaacatttacgcagatcgtttctgtcgtcaccgaacaaatccagagccgcgtcgttaacaatgtcgactatgagttgttatgccgggaacgcgataacttccgcatcctggtcgccatcaccaacgcggtgctttcccgcctggatatggacgaactggtcagcgaagtcgccaaagaaatccattactatttcgacattgacgatatcagtatcgtcttacgcagccaccgtaaaaacaaactcaacatctactccactcactatcttgataaacagcatcccgcccacgaacagagcgaagtcgatgaagccggaaccctcaccgaacgcgtgttcaaaagtaaagagatgctgctgatcaatctccacgagcgggacgatttagccccctatgaacgcatgttgttcgacacctggggcaaccagattcaaaccttgtgcctgttaccgctgatgtctggcgacaccatgctgggcgtgctgaaactggcgcaatgcgaagagaaagtgtttaccactaccaatctgaatttactgcgccagattgccgaacgtgtggcaatcgctgtcgataacgccctcgcctatcaggaaatccatcgtctgaaagaacggctggttgatggaaacctcgccctgaccgagcagctcaacaatgttgatagtgaatttggcgagattattggccgcagcgaagccatgtacagcgtgcttaaacaagttgaaatggtggcgcaaagtgacagtaccgtgctgatcctcggtgaaactggcacgggtaaagagctgattgcccgtgcgatccataatctcagtgggcgtaataatcgccgcatggtcaaaatgaactgcgcggcgatgcctgccggattgctggaaagcgatctgtttggtcatgagcgtggggcttttaccggtgccagcgcccagcgtatcggtcgttttgaactggcggataaaagctccctgttcctcgacgaagtgggcgatatgccactggagttacagccgaagttgctgcgtgtattgcaggaacaggagtttgaacgtctcggcagcaacaaaatcattcagacggacgtgcgtctaatcgccgcgactaaccgcgatctgaaaaaaatggtcgccgaccgtgagttccgtagcgatctctattaccgcctgaacgtattcccgattcacctgccgccactacgcgagcgtccggaagatattccgctgctggcgaaagcctttaccttcaaaattgcccgtcgtctggggcgcaatatcgacagcattcctgccgagacgctgcgcaccttgagcaacatggagtggccgggtaacgtacgcgaactggaaaacgtcattgagcgcgcggtattgctaacacgcggtaacgtgctgcaactgtcattgccagatattgttttaccggaacctgaaacgccgcctgccgcagcggttgtcgccctggagggcgaagatgaatatcagttgattgtgcgcgtgctgaaagaaaccaacggcgtggttgccgggcctaaaggcgctgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z