LOCUS       BBa_K779607              939 bp    DNA     linear       23-Jan-2026
DEFINITION  Tre-Tight-mKate MammoBlock Device
ACCESSION   BBa_K779607
VERSION     BBa_K779607.1  
FEATURES             Location/Qualifiers
     promoter        1..196
                     /label=TRE-tight
     misc_feature    1..18
                     /label=Tet-O (1)
     misc_feature    36..53
                     /label=Tet-O (2)
     misc_feature    72..89
                     /label=Tet-O (3)
     misc_feature    108..125
                     /label=Tet-O (4)
     misc_feature    143..160
                     /label=Tet-O (5)
     misc_feature    179..196
                     /label=Tet-O (6)
     promoter        1..196
                     /label=BBa_K779202
     misc_feature    198..218
                     /label=attB1
     misc_feature    197..218
                     /label=BBa_J85600
     misc_feature    219..229
                     /label=Kozak
     CDS             226..939
                     /label=mKate
     misc_feature    219..939
                     /label=BBa_K779304
ORIGIN
        1 ccctatcagt gatagagaac gatgtcgagt ttactcccta tcagtgatag agaacgtatg
       61 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      121 agagaacgta tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc
      181 ctatcagtga tagagaccaa gtttgtacaa aaaagcagcg ccaccatggt gtctaagggc
      241 gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac
      301 caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg
      361 agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc
      421 ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag
      481 cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg
      541 ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc
      601 agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag
      661 gcctccaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc
      721 ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata cagatccaag
      781 aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag actggaaaga
      841 atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac
      901 tgcgacctcc ctagcaaact ggggcacaaa cttaattga
//