BBa_K781000 1 BBa_K781000 [R0010][B0034] - E. coli K12 FliC 2012-09-30T11:00:00Z 2015-05-08T01:13:18Z The first constant domains and the D3 domain were amplified out of the separately, incorporating the appropriate silent mutations. The second constant domains were synthesized. The total coding domain was assembled using overlap extension PCR. This is the complete flagellin coding sequence with biobrick standard cut sites removed, under the promoter R0010 and RBS B0034. This part can be used to over express the flagellin monomer. Or, this part can be used as a template for PCR overlap extension, which will make an insertion at the annotated site, given that the insert is in the appropriate format. The resulting chimera will express protein immediately after the PCR reaction is transformed. false false _1035_ 0 9715 9 It's complicated true Note that when expressing this, and resulting overlaps in bacteria. One should be aware that this promoter is sensitive to LacI. Any strains overly expressing LacI will have very little expression of protein. false Kevin Chen annotation2205349 1 conserved range2205349 1 213 216 annotation2210572 1 E. coli Flagellin range2210572 1 221 1826 annotation2205342 1 BBa_R0010 range2205342 1 1 200 annotation2205344 1 CAP binding site range2205344 1 89 126 annotation2205343 1 end of LacI coding region (inactive) range2205343 1 1 88 annotation2205347 1 LacI binding site range2205347 1 166 200 annotation2205346 1 -10 range2205346 1 161 166 annotation2205348 1 start range2205348 1 173 173 annotation2205350 1 BBa_B0034 range2205350 1 209 220 annotation2205345 1 -35 range2205345 1 137 142 BBa_K781000_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccacaagtgctccagtaactgcttttggtgctaccaccacaaacaatattaaacttactggaattaccctttctacggaagcagccactgatactggcggaactaacccagcttcaattgagggtgtttatactgataatggtaatgattactatgcgaaaatcaccggtggtgataacgatgggaagtattacgcagtaacagttgctaatgatggtacagtgacaatggcgactggagcaacggcaaatgcaactgtaactgatgcaaatactactaaagctacaactatcacttcaggcggtacacctgttcagattgataatacagcaggttccgcaactgccaaccttggtgctgttagcttagtaaaattgcaggattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttgcagggttaatcgttgtaacctgattaactgagactgacggcaacgccaaattgcctgatgcgctgcgcttatcaggcctacaagttgaattgcaatttattgaatttgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z