LOCUS       BBa_K781001             2446 bp    DNA     linear       11-May-2024
DEFINITION  [R0010][B0034] - RFP-FliC Insertion Chimera
ACCESSION   BBa_K781001
VERSION     BBa_K781001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    89..126
                     /label=CAP
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI
     misc_feature    173..173
                     /label=start
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    213..216
                     /label=conserved
     CDS             230..975
                     /label=Flagellin
     primer_bind     946..976
                     /label=1st Overlapping region
     CDS             977..996
                     /label=Linker
     misc_feature    997..1672
                     /label=BBa_E1010
     CDS             1673..1692
                     /label=Linker 2
     CDS             1693..2443
                     /label=Flagellin
     primer_bind     1693..1721
                     /label=2nd Overlapping region
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa atactagaga tggcacaagt
      241 cattaatacc aacagcctct cgctgatcac tcaaaataat atcaacaaga accagtctgc
      301 gctgtcgagt tctatcgagc gtctgtcttc tggcttgcgt attaacagcg cgaaggatga
      361 cgcagcgggt caggcgattg ctaaccgttt cacctctaac attaaaggcc tgactcaggc
      421 ggcccgtaac gccaacgacg gtatctccgt tgcgcagacc accgaaggcg cgctgtccga
      481 aatcaacaac aacttacagc gtgtgcgtga actgacggta caggccacta ccggtactaa
      541 ctctgagtct gatctgtctt ctatccagga cgaaattaaa tcccgtctgg atgaaattga
      601 ccgcgtatct ggtcagaccc agttcaacgg cgtgaacgtg ctggcaaaaa atggctccat
      661 gaaaatccag gttggcgcaa atgataacca gactatcact atcgatctga agcagattga
      721 tgctaaaact cttggccttg atggttttag cgttaaaaat aacgatacag ttaccacaag
      781 tgctccagta actgcttttg gtgctaccac cacaaacaat attaaactta ctggaattac
      841 cctttctacg gaagcagcca ctgatactgg cggaactaac ccagcttcaa ttgagggtgt
      901 ttatactgat aatggtaatg attactatgc gaaaatcacc ggtggtgata acgatgggaa
      961 gtattacgca gtaacaacca caggaggtgg aggttcaatg gcttcctccg aagacgttat
     1021 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
     1081 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
     1141 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
     1201 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
     1261 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
     1321 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
     1381 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
     1441 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
     1501 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
     1561 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
     1621 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg ctggtggatc
     1681 aggtggaact tcaacagtga caatggcgac tggagcaacg gcaaatgcac atcatcacca
     1741 tcaccacact gtaactgatg caaatactac taaagctaca actatcactt caggcggtac
     1801 acctgttcag attgataata cagcaggttc cgcaactgcc aaccttggtg ctgttagctt
     1861 agtaaaattg caggattcca agggtaatga taccgataca tatgcgctta aagatacaaa
     1921 tggcaatctt tacgctgcgg atgtgaatga aactactggt gctgtttctg ttaaaactat
     1981 tacctatact gactcttccg gtgccgccag ttctccaacc gcggtcaaac tgggcggaga
     2041 tgatggcaaa acagaagtgg tcgatattga tggtaaaaca tacgattctg ccgatttaaa
     2101 tggcggtaat ctgcaaacag gtttgactgc tggtggtgag gctctgactg ctgttgcaaa
     2161 tggtaaaacc acggatccgc tgaaagcgct ggacgatgct atcgcatctg tagacaaatt
     2221 ccgttcttcc ctcggtgcgg tgcaaaaccg tctggattcc gcggttacca acctgaacaa
     2281 caccactacc aacctgtctg aagcgcagtc ccgtattcag gacgccgact atgcgaccga
     2341 agtgtccaat atgtcgaaag cgcagatcat ccagcaggcc ggtaactccg tgttggcaaa
     2401 agctaaccag gtaccgcagc aggttctgtc tctgttgcag ggttaa
//