LOCUS       BBa_K781003             2291 bp    DNA     linear       21-May-2024
DEFINITION  [R0010][B0034] - Cohesin II-FliC Insertion Chimera
ACCESSION   BBa_K781003
VERSION     BBa_K781003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     misc_feature    213..216
                     /label=conserved
     misc_feature    209..220
                     /label=BBa_B0034
     CDS             230..975
                     /label=Flagellin
     CDS             1538..2288
                     /label=Flagellin
     CDS             998..1517
                     /label=Cohesin II
     misc_feature    998..998
                     /label=Frame shift error
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa atactagaga tggcacaagt
      241 cattaatacc aacagcctct cgctgatcac tcaaaataat atcaacaaga accagtctgc
      301 gctgtcgagt tctatcgagc gtctgtcttc tggcttgcgt attaacagcg cgaaggatga
      361 cgcagcgggt caggcgattg ctaaccgttt cacctctaac attaaaggcc tgactcaggc
      421 ggcccgtaac gccaacgacg gtatctccgt tgcgcagacc accgaaggcg cgctgtccga
      481 aatcaacaac aacttacagc gtgtgcgtga actgacggta caggccacta ccggtactaa
      541 ctctgagtct gatctgtctt ctatccagga cgaaattaaa tcccgtctgg atgaaattga
      601 ccgcgtatct ggtcagaccc agttcaacgg cgtgaacgtg ctggcaaaaa atggctccat
      661 gaaaatccag gttggcgcaa atgataacca gactatcact atcgatctga agcagattga
      721 tgctaaaact cttggccttg atggttttag cgttaaaaat aacgatacag ttaccacaag
      781 tgctccagta actgcttttg gtgctaccac cacaaacaat attaaactta ctggaattac
      841 cctttctacg gaagcagcca ctgatactgg cggaactaac ccagcttcaa ttgagggtgt
      901 ttatactgat aatggtaatg attactatgc gaaaatcacc ggtggtgata acgatgggaa
      961 gtattacgca gtaacaacca caggaggtgg aggttcaggc agataaagcc tcgagcattg
     1021 agcttaagtt tgaccgcaat aagggagaag ttggagatat acttattggt accgtaagga
     1081 taaacaatat caagaatttc gcaggatttc aggtaaacat tgtatatgat ccaaaagtct
     1141 taatggctgt tgaccctgaa acggggaaag aatttacttc ttcaacattt ccgccaggac
     1201 gcactgtact gaaaaacaat gcttacggcc caatacagat tgcggacaat gatccggaaa
     1261 aagggatact gaacttcgcg cttgcatatt catatattgc gggatacaaa gaaacaggag
     1321 tagcggagga aagcggcata attgcgaaaa ttggatttaa aatactccag aaaaagagca
     1381 ctgccgtaaa attccaggat acattaagca tgcccggagc tatttcggga acacagctgt
     1441 ttgactggga cggagaagtt attaccggat atgaggtaat acagccggat gtgctgagtt
     1501 tgggtgacga gccttatggt ggatcaggtg gaacttcaac agtgacaatg gcgactggag
     1561 caacggcaaa tgcacatcat caccatcacc acactgtaac tgatgcaaat actactaaag
     1621 ctacaactat cacttcaggc ggtacacctg ttcagattga taatacagca ggttccgcaa
     1681 ctgccaacct tggtgctgtt agcttagtaa aattgcagga ttccaagggt aatgataccg
     1741 atacatatgc gcttaaagat acaaatggca atctttacgc tgcggatgtg aatgaaacta
     1801 ctggtgctgt ttctgttaaa actattacct atactgactc ttccggtgcc gccagttctc
     1861 caaccgcggt caaactgggc ggagatgatg gcaaaacaga agtggtcgat attgatggta
     1921 aaacatacga ttctgccgat ttaaatggcg gtaatctgca aacaggtttg actgctggtg
     1981 gtgaggctct gactgctgtt gcaaatggta aaaccacgga tccgctgaaa gcgctggacg
     2041 atgctatcgc atctgtagac aaattccgtt cttccctcgg tgcggtgcaa aaccgtctgg
     2101 attccgcggt taccaacctg aacaacacca ctaccaacct gtctgaagcg cagtcccgta
     2161 ttcaggacgc cgactatgcg accgaagtgt ccaatatgtc gaaagcgcag atcatccagc
     2221 aggccggtaa ctccgtgttg gcaaaagcta accaggtacc gcagcaggtt ctgtctctgt
     2281 tgcagggtta a
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