BBa_K781003 1 BBa_K781003 [R0010][B0034] - Cohesin II-FliC Insertion Chimera 2012-10-02T11:00:00Z 2015-05-08T01:13:18Z The cohesin portion was PCR amplified from a plasmid provided by Dr. Steven Smith of the Queen's University Department of Biomedical and Molecular Sciences. The amplified part was used for PCR overlap extension into the flagellin variable domain. This is type II cohesin from Clostridium Thermocellum as a chimeric insertion into flagellin. false false _1035_ 0 9715 9 It's complicated false The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera. false Kevin Chen annotation2209376 1 start range2209376 1 173 173 annotation2212096 1 Flagellin range2212096 1 1538 2288 annotation2209372 1 CAP binding site range2209372 1 89 126 annotation2209373 1 -35 range2209373 1 137 142 annotation2209374 1 -10 range2209374 1 161 166 annotation2209370 1 BBa_R0010 range2209370 1 1 200 annotation2209375 1 LacI binding site range2209375 1 166 200 annotation2209378 1 BBa_B0034 range2209378 1 209 220 annotation2212095 1 Flagellin range2212095 1 230 975 annotation2209377 1 conserved range2209377 1 213 216 annotation2212097 1 Cohesin II range2212097 1 998 1517 annotation2329247 1 Frame shift error range2329247 1 998 998 annotation2209371 1 end of LacI coding region (inactive) range2209371 1 1 88 BBa_K781003_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaaatactagagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccacaagtgctccagtaactgcttttggtgctaccaccacaaacaatattaaacttactggaattaccctttctacggaagcagccactgatactggcggaactaacccagcttcaattgagggtgtttatactgataatggtaatgattactatgcgaaaatcaccggtggtgataacgatgggaagtattacgcagtaacaaccacaggaggtggaggttcaggcagataaagcctcgagcattgagcttaagtttgaccgcaataagggagaagttggagatatacttattggtaccgtaaggataaacaatatcaagaatttcgcaggatttcaggtaaacattgtatatgatccaaaagtcttaatggctgttgaccctgaaacggggaaagaatttacttcttcaacatttccgccaggacgcactgtactgaaaaacaatgcttacggcccaatacagattgcggacaatgatccggaaaaagggatactgaacttcgcgcttgcatattcatatattgcgggatacaaagaaacaggagtagcggaggaaagcggcataattgcgaaaattggatttaaaatactccagaaaaagagcactgccgtaaaattccaggatacattaagcatgcccggagctatttcgggaacacagctgtttgactgggacggagaagttattaccggatatgaggtaatacagccggatgtgctgagtttgggtgacgagccttatggtggatcaggtggaacttcaacagtgacaatggcgactggagcaacggcaaatgcacatcatcaccatcaccacactgtaactgatgcaaatactactaaagctacaactatcacttcaggcggtacacctgttcagattgataatacagcaggttccgcaactgccaaccttggtgctgttagcttagtaaaattgcaggattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttgcagggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z