LOCUS       BBa_K781004             2662 bp    DNA     linear       27-Dec-2024
DEFINITION  [R0010][B0034] - Rv2579 Dehalogenase-FliC Insertion Chimera
ACCESSION   BBa_K781004
VERSION     BBa_K781004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     misc_feature    213..216
                     /label=conserved
     misc_feature    209..220
                     /label=BBa_B0034
     CDS             230..976
                     /label=Flagellin
     CDS             977..997
                     /label=Linker 1
     CDS             998..1888
                     /label=Rv2579
     CDS             1889..1908
                     /label=Linker 2
     CDS             1909..2659
                     /label=Flagellin
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa atactagaga tggcacaagt
      241 cattaatacc aacagcctct cgctgatcac tcaaaataat atcaacaaga accagtctgc
      301 gctgtcgagt tctatcgagc gtctgtcttc tggcttgcgt attaacagcg cgaaggatga
      361 cgcagcgggt caggcgattg ctaaccgttt cacctctaac attaaaggcc tgactcaggc
      421 ggcccgtaac gccaacgacg gtatctccgt tgcgcagacc accgaaggcg cgctgtccga
      481 aatcaacaac aacttacagc gtgtgcgtga actgacggta caggccacta ccggtactaa
      541 ctctgagtct gatctgtctt ctatccagga cgaaattaaa tcccgtctgg atgaaattga
      601 ccgcgtatct ggtcagaccc agttcaacgg cgtgaacgtg ctggcaaaaa atggctccat
      661 gaaaatccag gttggcgcaa atgataacca gactatcact atcgatctga agcagattga
      721 tgctaaaact cttggccttg atggttttag cgttaaaaat aacgatacag ttaccacaag
      781 tgctccagta actgcttttg gtgctaccac cacaaacaat attaaactta ctggaattac
      841 cctttctacg gaagcagcca ctgatactgg cggaactaac ccagcttcaa ttgagggtgt
      901 ttatactgat aatggtaatg attactatgc gaaaatcacc ggtggtgata acgatgggaa
      961 gtattacgca gtaacaacca caggaggtgg aggttcaatg acagcattcg gcgtcgagcc
     1021 ctacgggcag ccgaagtacc tagaaatcgc cgggaagcgc atggcgtata tcgacgaagg
     1081 caagggtgac gccatcgtct ttcagcacgg caaccccacg tcgtcttact tgtggcgcaa
     1141 catcatgccg cacttggaag ggctgggccg gctggtggcc tgcgatctga tcgggatggg
     1201 cgcgtcggac aagctcagcc catcgggacc cgaccgctat agctatggcg agcaacgaga
     1261 ctttttgttc gcgctctggg atgcgctcga cctcggcgac cacgtggtac tggtgctgca
     1321 cgactggggc tcggcgctcg gcttcgactg ggctaaccag catcgcgacc gagtgcaggg
     1381 gatcgcgttc atggaagcga tcgtcacccc gatgacgtgg gcggactggc cgccggccgt
     1441 gcggggtgtg ttccagggtt tccgatcgcc tcaaggcgag ccaatggcgt tggagcacaa
     1501 catctttgtc gaacgggtgc tgcccggggc gatcctgcga cagctcagcg acgaggaaat
     1561 gaaccactat cggcggccat tcgtgaacgg cggcgaggac cgtcgcccca cgttgtcgtg
     1621 gccacgaaac cttccaatcg acggtgagcc cgccgaggtc gtcgcgttgg tcaacgagta
     1681 ccggagctgg ctcgaggaaa ccgacatgcc gaaactgttc atcaacgccg agcccggcgc
     1741 gatcatcacc ggccgcatcc gtgactatgt caggagctgg cccaaccaga ccgaaatcac
     1801 agtgcccggc gtgcatttcg ttcaggagga cagcccagag gaaatcggtg cggccatagc
     1861 acagttcgtc cggcggctcc ggtcggcggg tggatcaggt ggaacttcaa cagtgacaat
     1921 ggcgactgga gcaacggcaa atgcacatca tcaccatcac cacactgtaa ctgatgcaaa
     1981 tactactaaa gctacaacta tcacttcagg cggtacacct gttcagattg ataatacagc
     2041 aggttccgca actgccaacc ttggtgctgt tagcttagta aaattgcagg attccaaggg
     2101 taatgatacc gatacatatg cgcttaaaga tacaaatggc aatctttacg ctgcggatgt
     2161 gaatgaaact actggtgctg tttctgttaa aactattacc tatactgact cttccggtgc
     2221 cgccagttct ccaaccgcgg tcaaactggg cggagatgat ggcaaaacag aagtggtcga
     2281 tattgatggt aaaacatacg attctgccga tttaaatggc ggtaatctgc aaacaggttt
     2341 gactgctggt ggtgaggctc tgactgctgt tgcaaatggt aaaaccacgg atccgctgaa
     2401 agcgctggac gatgctatcg catctgtaga caaattccgt tcttccctcg gtgcggtgca
     2461 aaaccgtctg gattccgcgg ttaccaacct gaacaacacc actaccaacc tgtctgaagc
     2521 gcagtcccgt attcaggacg ccgactatgc gaccgaagtg tccaatatgt cgaaagcgca
     2581 gatcatccag caggccggta actccgtgtt ggcaaaagct aaccaggtac cgcagcaggt
     2641 tctgtctctg ttgcagggtt aa
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