LOCUS       BBa_K781005             2656 bp    DNA     linear       29-Jun-2024
DEFINITION  [R0010][B0034] - LinB Dehalogenase-FliC Insertion Chimera
ACCESSION   BBa_K781005
VERSION     BBa_K781005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     misc_feature    213..216
                     /label=conserved
     misc_feature    209..220
                     /label=BBa_B0034
     CDS             230..976
                     /label=Flagellin
     CDS             977..997
                     /label=Linker 1
     CDS             998..1882
                     /label=LinB
     CDS             1883..1902
                     /label=Linker 2
     CDS             1903..2653
                     /label=Flagellin
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa atactagaga tggcacaagt
      241 cattaatacc aacagcctct cgctgatcac tcaaaataat atcaacaaga accagtctgc
      301 gctgtcgagt tctatcgagc gtctgtcttc tggcttgcgt attaacagcg cgaaggatga
      361 cgcagcgggt caggcgattg ctaaccgttt cacctctaac attaaaggcc tgactcaggc
      421 ggcccgtaac gccaacgacg gtatctccgt tgcgcagacc accgaaggcg cgctgtccga
      481 aatcaacaac aacttacagc gtgtgcgtga actgacggta caggccacta ccggtactaa
      541 ctctgagtct gatctgtctt ctatccagga cgaaattaaa tcccgtctgg atgaaattga
      601 ccgcgtatct ggtcagaccc agttcaacgg cgtgaacgtg ctggcaaaaa atggctccat
      661 gaaaatccag gttggcgcaa atgataacca gactatcact atcgatctga agcagattga
      721 tgctaaaact cttggccttg atggttttag cgttaaaaat aacgatacag ttaccacaag
      781 tgctccagta actgcttttg gtgctaccac cacaaacaat attaaactta ctggaattac
      841 cctttctacg gaagcagcca ctgatactgg cggaactaac ccagcttcaa ttgagggtgt
      901 ttatactgat aatggtaatg attactatgc gaaaatcacc ggtggtgata acgatgggaa
      961 gtattacgca gtaacaacca caggaggtgg aggttcaatg agcctcggcg caaagccatt
     1021 tggcgagaag aaattcattg agatcaaggg ccggcgcatg gcctatatcg atgaagggac
     1081 cggcgatccg atcctcttcc agcacggcaa tccgacgtcg tcctatctgt ggcgcaatat
     1141 catgccgcat tgcgccgggc tgggacggct gatcgcctgt gacctgatcg gcatgggcga
     1201 ttcggacaag ctcgatccgt cggggcccga gcgttatgcc tatgccgagc atcgtgacta
     1261 tctcgacgcg ctgtgggagg cgctcgatct cggggacagg gttgttctgg tcgtgcatga
     1321 ctgggggtcc gccctcggct tcgactgggc ccgccgccac cgcgagcgtg tacaggggat
     1381 tgcctatatg gaagcgatcg ccatgccgat cgaatgggcg gattttcccg aacaggatcg
     1441 cgatctgttt caggcctttc gctcgcaggc gggcgaagaa ttggtgttgc aggacaatgt
     1501 ttttgtcgaa caagttctcc ccggattgat cctgcgcccc ttaagcgaag cggagatggc
     1561 cgcctatcgc gagcccttcc tcgccgccga agcccgtcga ccgaccctgt cttggcctcg
     1621 ccaaatcccg atcgcaggca ccccggccga cgtggtcgcg atcgcccggg actatgccgg
     1681 ctggctcagc gaaagcccga ttccgaaact cttcatcaac gccgagccgg gagccctgac
     1741 cacgggccga atgcgcgact tctgccgcac atggccaaac cagaccgaaa tcacggtcgc
     1801 aggcgcccat ttcatccagg aggacagtcc ggacgagatt ggcgcggcga ttgcggcgtt
     1861 tgtccggcga ttgcgcccag caggtggatc aggtggaact tcaacagtga caatggcgac
     1921 tggagcaacg gcaaatgcac atcatcacca tcaccacact gtaactgatg caaatactac
     1981 taaagctaca actatcactt caggcggtac acctgttcag attgataata cagcaggttc
     2041 cgcaactgcc aaccttggtg ctgttagctt agtaaaattg caggattcca agggtaatga
     2101 taccgataca tatgcgctta aagatacaaa tggcaatctt tacgctgcgg atgtgaatga
     2161 aactactggt gctgtttctg ttaaaactat tacctatact gactcttccg gtgccgccag
     2221 ttctccaacc gcggtcaaac tgggcggaga tgatggcaaa acagaagtgg tcgatattga
     2281 tggtaaaaca tacgattctg ccgatttaaa tggcggtaat ctgcaaacag gtttgactgc
     2341 tggtggtgag gctctgactg ctgttgcaaa tggtaaaacc acggatccgc tgaaagcgct
     2401 ggacgatgct atcgcatctg tagacaaatt ccgttcttcc ctcggtgcgg tgcaaaaccg
     2461 tctggattcc gcggttacca acctgaacaa caccactacc aacctgtctg aagcgcagtc
     2521 ccgtattcag gacgccgact atgcgaccga agtgtccaat atgtcgaaag cgcagatcat
     2581 ccagcaggcc ggtaactccg tgttggcaaa agctaaccag gtaccgcagc aggttctgtc
     2641 tctgttgcag ggttaa
//