BBa_K781005 1 BBa_K781005 [R0010][B0034] - LinB Dehalogenase-FliC Insertion Chimera 2012-10-02T11:00:00Z 2015-05-08T01:13:18Z This part was amplified from a plasmid provided to us by another research lab. It was then used for PCR overlap extension into the flagellin variable domain using the part K781000 as a template. Released HQ 2013 LinB is a dehalogenase enzyme. It degrades haloalkanes which are toxic pollutants that accumulate in food chains. This part has the LinB incorporated at an insertion site. false false _1035_ 0 9715 9 It's complicated false The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera. false Kevin Chen annotation2329254 1 Linker 1 range2329254 1 977 997 annotation2209441 1 CAP binding site range2209441 1 89 126 annotation2329257 1 Flagellin range2329257 1 1903 2653 annotation2209445 1 start range2209445 1 173 173 annotation2209444 1 LacI binding site range2209444 1 166 200 annotation2209439 1 BBa_R0010 range2209439 1 1 200 annotation2329255 1 LinB range2329255 1 998 1882 annotation2209442 1 -35 range2209442 1 137 142 annotation2209446 1 conserved range2209446 1 213 216 annotation2209443 1 -10 range2209443 1 161 166 annotation2329253 1 Flagellin range2329253 1 230 976 annotation2329256 1 Linker 2 range2329256 1 1883 1902 annotation2209440 1 end of LacI coding region (inactive) range2209440 1 1 88 annotation2209447 1 BBa_B0034 range2209447 1 209 220 BBa_K781005_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaaatactagagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccacaagtgctccagtaactgcttttggtgctaccaccacaaacaatattaaacttactggaattaccctttctacggaagcagccactgatactggcggaactaacccagcttcaattgagggtgtttatactgataatggtaatgattactatgcgaaaatcaccggtggtgataacgatgggaagtattacgcagtaacaaccacaggaggtggaggttcaatgagcctcggcgcaaagccatttggcgagaagaaattcattgagatcaagggccggcgcatggcctatatcgatgaagggaccggcgatccgatcctcttccagcacggcaatccgacgtcgtcctatctgtggcgcaatatcatgccgcattgcgccgggctgggacggctgatcgcctgtgacctgatcggcatgggcgattcggacaagctcgatccgtcggggcccgagcgttatgcctatgccgagcatcgtgactatctcgacgcgctgtgggaggcgctcgatctcggggacagggttgttctggtcgtgcatgactgggggtccgccctcggcttcgactgggcccgccgccaccgcgagcgtgtacaggggattgcctatatggaagcgatcgccatgccgatcgaatgggcggattttcccgaacaggatcgcgatctgtttcaggcctttcgctcgcaggcgggcgaagaattggtgttgcaggacaatgtttttgtcgaacaagttctccccggattgatcctgcgccccttaagcgaagcggagatggccgcctatcgcgagcccttcctcgccgccgaagcccgtcgaccgaccctgtcttggcctcgccaaatcccgatcgcaggcaccccggccgacgtggtcgcgatcgcccgggactatgccggctggctcagcgaaagcccgattccgaaactcttcatcaacgccgagccgggagccctgaccacgggccgaatgcgcgacttctgccgcacatggccaaaccagaccgaaatcacggtcgcaggcgcccatttcatccaggaggacagtccggacgagattggcgcggcgattgcggcgtttgtccggcgattgcgcccagcaggtggatcaggtggaacttcaacagtgacaatggcgactggagcaacggcaaatgcacatcatcaccatcaccacactgtaactgatgcaaatactactaaagctacaactatcacttcaggcggtacacctgttcagattgataatacagcaggttccgcaactgccaaccttggtgctgttagcttagtaaaattgcaggattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttgcagggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z