BBa_K781006 1 BBa_K781006 [R0010][B0034] - RFP-FliC Deletion Chimera 2012-10-02T11:00:00Z 2015-05-08T01:13:18Z This part was PCR amplified from J04450 and then overlap extension was used to incorporate it into the plasmid. This is the modified RFP protein obtained from J04450, that has been incorporated into the E. coli flagellin as a deletion. This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies. false false _1035_ 0 9715 9 In stock true The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera. Note that there are two different formats for making flagellin insertion variants and deletion variants false Kevin Chen annotation2209455 1 -10 range2209455 1 161 166 annotation2209456 1 LacI binding site range2209456 1 166 200 annotation2211302 1 BBa_E1010 range2211302 1 808 1482 annotation2209458 1 conserved range2209458 1 213 216 annotation2209457 1 start range2209457 1 173 173 annotation2209453 1 CAP binding site range2209453 1 89 126 annotation2211304 1 Flagellin range2211304 1 1496 2154 annotation2329258 1 Linker 1 range2329258 1 793 807 annotation2329259 1 Linker 2 range2329259 1 1483 1497 annotation2209454 1 -35 range2209454 1 137 142 annotation2211303 1 Flagellin range2211303 1 229 792 annotation2209451 1 BBa_R0010 range2209451 1 1 200 annotation2209452 1 end of LacI coding region (inactive) range2209452 1 1 88 annotation2209459 1 BBa_B0034 range2209459 1 209 220 BBa_K781006_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccacaggtgctaccaccacaggaggtggaggttcaatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctggtggatcaggtggaacttcaggcggtacacctgttcagattgataatacagcaggttccgcaactgccaaccttggtgctgttagcttagtaaaattgcaggattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttgcagggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z