BBa_K781007
1
BBa_K781007
[R0010][B0034] - XylE-FliC Deletion Chimera
2012-10-02T11:00:00Z
2015-05-08T01:13:18Z
The original XylE came from a biobrick part. It was amplified using PCR and then overlap extension was used to incorporate it into the flagella.
This is an XylE deletion variant of the flagellin monomer. The enzyme XylE that degrades catechol was incorporated into the E. coli flagellin by replacing the variable domain.
false
false
_1035_
0
9715
9
It's complicated
false
When amplifying this part, the overlapping regions were carefully chosen so that the insert sequence alone can be inforporated into a plasmid, for future use in overlap extension. Digesting with the two inner enzymes for the BB-2 enzymes S and N, leaves the appropriate sequence for overlap extension.
false
Kevin Chen
annotation2211317
1
K147002
range2211317
1
808
1728
annotation2209467
1
conserved
range2209467
1
213
216
annotation2211316
1
Flagellin
range2211316
1
229
777
annotation2209468
1
BBa_B0034
range2209468
1
209
220
annotation2209460
1
BBa_R0010
range2209460
1
1
200
annotation2211318
1
Flagellin
range2211318
1
1759
2403
annotation2329261
1
Linker 2
range2329261
1
1729
1758
annotation2209463
1
-35
range2209463
1
137
142
annotation2209464
1
-10
range2209464
1
161
166
annotation2329260
1
Linker 1
range2329260
1
778
807
annotation2209466
1
start
range2209466
1
173
173
annotation2209461
1
end of LacI coding region (inactive)
range2209461
1
1
88
annotation2209465
1
LacI binding site
range2209465
1
166
200
annotation2209462
1
CAP binding site
range2209462
1
89
126
BBa_K781007_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagatggcacaagtcattaataccaacagcctctcgctgatcactcaaaataatatcaacaagaaccagtctgcgctgtcgagttctatcgagcgtctgtcttctggcttgcgtattaacagcgcgaaggatgacgcagcgggtcaggcgattgctaaccgtttcacctctaacattaaaggcctgactcaggcggcccgtaacgccaacgacggtatctccgttgcgcagaccaccgaaggcgcgctgtccgaaatcaacaacaacttacagcgtgtgcgtgaactgacggtacaggccactaccggtactaactctgagtctgatctgtcttctatccaggacgaaattaaatcccgtctggatgaaattgaccgcgtatctggtcagacccagttcaacggcgtgaacgtgctggcaaaaaatggctccatgaaaatccaggttggcgcaaatgataaccagactatcactatcgatctgaagcagattgatgctaaaactcttggccttgatggttttagcgttaaaaataacgatacagttaccacaggtgctaccaccacaggaggtggaggttcaatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgaccggtggatcaggtggaacttcaggcggtacacctgttcagattgataatacagcaggttccgcaactgccaaccttggtgctgttagcttagtaaaattgcaggattccaagggtaatgataccgatacatatgcgcttaaagatacaaatggcaatctttacgctgcggatgtgaatgaaactactggtgctgtttctgttaaaactattacctatactgactcttccggtgccgccagttctccaaccgcggtcaaactgggcggagatgatggcaaaacagaagtggtcgatattgatggtaaaacatacgattctgccgatttaaatggcggtaatctgcaaacaggtttgactgctggtggtgaggctctgactgctgttgcaaatggtaaaaccacggatccgctgaaagcgctggacgatgctatcgcatctgtagacaaattccgttcttccctcggtgcggtgcaaaaccgtctggattccgcggttaccaacctgaacaacaccactaccaacctgtctgaagcgcagtcccgtattcaggacgccgactatgcgaccgaagtgtccaatatgtcgaaagcgcagatcatccagcaggccggtaactccgtgttggcaaaagctaaccaggtaccgcagcaggttctgtctctgttgcagggttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z