LOCUS BBa_K782008 2861 bp DNA linear 29-Nov-2024 DEFINITION TALA:NLS:KRAB ACCESSION BBa_K782008 VERSION BBa_K782008.1 FEATURES Location/Qualifiers CDS 33..2445 /label=TAL A CDS 2446..2469 /label=NLS CDS 2470..2853 /label=KRAB misc_feature 15..32 /label=His tag RBS 6..12 /label=Kozak ORIGIN 1 aattcgccac catgcaccac caccaccacc acgactacaa agaccatgac ggtgattata 61 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga 121 aggtgggcat tcaccgcggg gtacctatgg tggacttgag gacactcggt tattcgcaac 181 agcaacagga gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc 241 ttgtggggca tggcttcact catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc 301 ttgggacggt ggctgtcaaa taccaagata tgattgcggc cctgcccgaa gccacgcacg 361 aggcaattgt aggggtcggt aaacagtggt cgggagcgcg agcacttgag gcgctgctga 421 ctgtggcggg tgagcttagg gggcctccgc tccagctcga caccgggcag ctgctgaaga 481 tcgcgaagag agggggagta acagcggtag aggcagtgca cgcctggcgc aatgcgctca 541 ccggggcccc cttgaacctg accccagacc aggtagtcgc aatcgcgtca aacggagggg 601 gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa gaccacggcc 661 ttacaccgga gcaagtcgtg gccattgcaa gcaatggggg tggcaaacag gctcttgaga 721 cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc gatcaagttg 781 tagcgattgc gtcgaacatt ggagggaaac aagcattgga gactgtccaa cggctccttc 841 ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc gccagccatg 901 atggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg tgccaggatc 961 atggactgac cccagaccag gtagtcgcaa tcgcgtcaaa cggaggggga aagcaagccc 1021 tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt acaccggagc 1081 aagtcgtggc cattgcaaat aataacggtg gcaaacaggc tcttgagacg gttcagagac 1141 ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta gcgattgcgt 1201 cgcatgacgg agggaaacaa gcattggaga ctgtccaacg gctccttccc gtgttgtgtc 1261 aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc ctcgaatggc ggcggtaagc 1321 aggcgctgga aacagtacag cgcctgctgc ctgtactgtg ccaggatcat ggactgaccc 1381 cagaccaggt agtcgcaatc gcgaacaata atgggggaaa gcaagccctg gaaaccgtgc 1441 aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa gtcgtggcca 1501 ttgcatccca cgacggtggc aaacaggctc ttgagacggt tcagagactt ctcccagttc 1561 tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcc aacggtggag 1621 ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa gcccacggtt 1681 tgacgcctgc acaagtggtc gccatcgcca gccatgatgg cggtaagcag gcgctggaaa 1741 cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca gaccaggtag 1801 tcgcaatcgc gtcacatgac gggggaaagc aagccctgga aaccgtgcaa aggttgttgc 1861 cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt gcatcccacg 1921 acggtggcaa acaggctctt gagacggttc agagacttct cccagttctc tgtcaagccc 1981 acgggctgac tcccgatcaa gttgtagcga ttgcgaataa caatggaggg aaacaagcat 2041 tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg acgcctgcac 2101 aagtggtcgc catcgccagc catgatggcg gtaagcaggc gctggaaaca gtacagcgcc 2161 tgctgcctgt actgtgccag gatcatggac tgacacccga acaggtggtc gccattgctt 2221 ctaatggggg aggacggcca gccttggagt ccatcgtagc ccaattgtcc aggcccgatc 2281 ccgcgttggc tgcgttaacg aatgaccatc tggtggcgtt ggcatgtctt ggtggacgac 2341 ccgcgctcga tgcagtcaaa aagggtctgc ctcatgctcc cgcattgatc aaaagaacca 2401 accggcggat tcccgagaga acttcccatc gagtcgcggg atccgatcca aaaaagaaga 2461 gaaaggtacc aaaaaagaag agaaaggtcg acggcggtgg tgctttgtct cctcagcact 2521 ctgctgtcac tcaaggaagt atcatcaaga acaaggaggg catggatgct aagtcactaa 2581 ctgcctggtc ccggacactg gtgaccttca aggatgtatt tgtggacttc accagggagg 2641 agtggaagct gctggacact gctcagcaga tcgtgtacag aaatgtgatg ctggagaact 2701 ataagaacct ggtttccttg ggttatcagc ttactaagcc agatgtgatc ctccggttgg 2761 agaagggaga agagccctgg ctggtggaga gagaaattca ccaagagacc catcctgatt 2821 cagagactgc atttgaaatc aaatcatcag tttaatctag g //