LOCUS       BBa_K782011             2555 bp    DNA     linear       23-Nov-2024
DEFINITION  NicTAL12:NLS:KRAB
ACCESSION   BBa_K782011
VERSION     BBa_K782011.1  
FEATURES             Location/Qualifiers
     RBS             6..11
                     /label=Kozak
     misc_feature    15..32
                     /label=His tag
     CDS             33..2138
                     /label=NicTAL12
     CDS             2139..2162
                     /label=NLS
     CDS             2163..2546
                     /label=KRAB
ORIGIN
        1 aattcgccac catgcaccac caccaccacc acgactacaa agaccatgac ggtgattata
       61 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga
      121 aggtgggcat tcaccgcggg gtacctatgg tggacttgag gacactcggt tattcgcaac
      181 agcaacagga gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc
      241 ttgtggggca tggcttcact catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc
      301 ttgggacggt ggctgtcaaa taccaagata tgattgcggc cctgcccgaa gccacgcacg
      361 aggcaattgt aggggtcggt aaacagtggt cgggagcgcg agcacttgag gcgctgctga
      421 ctgtggcggg tgagcttagg gggcctccgc tccagctcga caccgggcag ctgctgaaga
      481 tcgcgaagag agggggagta acagcggtag aggcagtgca cgcctggcgc aatgcgctca
      541 ccggggcccc gctgaatctg acaccggaac aggttgttgc aattgcaagc catgatggtg
      601 gtaaacaggc actggaaacc gttcagcgtc tgctgccggt tctgtgtcag gcacatggtc
      661 tgacccctga acaggtggtg gccattgcca gtaatggtgg tggcaaacag gcgttagaaa
      721 cagtgcagcg cctgctgcct gttttatgcc aggcccatgg cctgacacca gagcaggtag
      781 tggcgattgc gagcaatatt ggcggtaaac aagcccttga aaccgtgcag gcattactgc
      841 cggtgctgtg ccaagcgcac ggcctgaccc cagaacaagt tgttgcgatc gcatcaaatg
      901 gtggcggtaa gcaggctttg gaaacggtgc agcgtttact gccagtgtta tgtcaggcgc
      961 atggtttaac accggaacaa gtggtggcaa tcgcctcaca tgatggcgga aaacaagcat
     1021 tagagactgt tcaacgcctg ctgccagtgc tttgccaggc acacggctta acacctgaac
     1081 aggtcgtagc gattgcatca aatattggtg gaaaacaggc cttggagact gtacaggcac
     1141 tgctgcctgt actgtgtcaa gcccacggat taacgccaga acaggttgtg gctatcgcca
     1201 gcaattcagg cggtaaacag gcgctcgaga cagttcaggc cctgctgccg gtcttatgtc
     1261 aagctcacgg actgactccc gagcaggttg tcgccattgc ctcaaatggc ggtggtaaac
     1321 aagctctcga aacggtacag agactgctgc ccgtcctgtg ccaggcgcat ggacttacgc
     1381 ctgagcaagt tgtggcaatt gcatctaata atgggggtaa gcaagcgctg gaaacagttc
     1441 aacgcttact gcctgtcttg tgccaagcac atggtttaac cccagagcag gtcgttgcta
     1501 ttgcctctaa cattggaggc aaacaggctc ttgaaactgt ccaggccctg ttacctgttc
     1561 tgtgccaggc tcacggtttg actccagagc aagtggttgc aatagccagc aacggtgggg
     1621 gtaaacaagc tttagaaacc gtccaacgtc tgttaccagt gctgtgtcaa gctcatggcc
     1681 ttacacccga acaagtagtt gccattgcga gtaacattgg tgggaagcaa gcacttgaaa
     1741 cggttcaggc gctgcttcca gtattatgcc aggcgcacgg actcactcca gaacaggtag
     1801 tagcaatcgc aagtaataac ggtgggaaac aagcgttgga gacagtccaa agactgcttc
     1861 cggttctttg ccaagcccac ggtcttacac cgcagcaggt tgtagctatt gctagtaata
     1921 ttggaggtcg tccggcactg gaaagcattg ttgcacagct gagccgtcct gatccggcac
     1981 tggcagcact gaccaatgat catctggttg cactggcatg tctgggtggt cgtcctgccc
     2041 tggatgcagt taaaaaaggt ctgccgcatg caccggcact gattaaacgt accaatcgtc
     2101 gtatcccgga acgtaccagc catcgtgttg caggatccga tccaaaaaag aagagaaagg
     2161 taccaaaaaa gaagagaaag gtcgacggcg gtggtgcttt gtctcctcag cactctgctg
     2221 tcactcaagg aagtatcatc aagaacaagg agggcatgga tgctaagtca ctaactgcct
     2281 ggtcccggac actggtgacc ttcaaggatg tatttgtgga cttcaccagg gaggagtgga
     2341 agctgctgga cactgctcag cagatcgtgt acagaaatgt gatgctggag aactataaga
     2401 acctggtttc cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagg
     2461 gagaagagcc ctggctggtg gagagagaaa ttcaccaaga gacccatcct gattcagaga
     2521 ctgcatttga aatcaaatca tcagtttaat ctagg
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