LOCUS       BBa_K782012             2612 bp    DNA     linear       01-Dec-2024
DEFINITION  TALD:NLS:VP16
ACCESSION   BBa_K782012
VERSION     BBa_K782012.1  
FEATURES             Location/Qualifiers
     RBS             6..11
                     /label=Kozak sequence
     misc_feature    15..32
                     /label=His tag
     CDS             33..2342
                     /label=TAL D
     CDS             2343..2366
                     /label=NLS
     CDS             2367..2603
                     /label=VP 16
ORIGIN
        1 aattcgccac catgcaccac caccaccacc acgactacaa agaccatgac ggtgattata
       61 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga
      121 aggtgggcat tcaccgcggg gtacctatgg tggacttgag gacactcggt tattcgcaac
      181 agcaacagga gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc
      241 ttgtggggca tggcttcact catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc
      301 ttgggacggt ggctgtcaaa taccaagata tgattgcggc cctgcccgaa gccacgcacg
      361 aggcaattgt aggggtcggt aaacagtggt cgggagcgcg agcacttgag gcgctgctga
      421 ctgtggcggg tgagcttagg gggcctccgc tccagctcga caccgggcag ctgctgaaga
      481 tcgcgaagag agggggagta acagcggtag aggcagtgca cgcctggcgc aatgcgctca
      541 ccggggcccc cttgaacctg accccagacc aggtagtcgc aatcgcgtca catgacgggg
      601 gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa gaccacggcc
      661 ttacaccgga gcaagtcgtg gccattgcaa ataataacgg tggcaaacag gctcttgaga
      721 cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc gatcaagttg
      781 tagcgattgc gtccaacggt ggagggaaac aagcattgga gactgtccaa cggctccttc
      841 ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc gccagccatg
      901 atggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg tgccaggatc
      961 atggactgac cccagaccag gtagtcgcaa tcgcgtcaca tgacggggga aagcaagccc
     1021 tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt acaccggagc
     1081 aagtcgtggc cattgcaagc aacatcggtg gcaaacaggc tcttgagacg gttcagagac
     1141 ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta gcgattgcgt
     1201 cgaacattgg agggaaacaa gcattggaga ctgtccaacg gctccttccc gtgttgtgtc
     1261 aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc ctcgaatggc ggcggtaagc
     1321 aggcgctgga aacagtacag cgcctgctgc ctgtactgtg ccaggatcat ggactgaccc
     1381 cagaccaggt agtcgcaatc gcgtcgaaca ttgggggaaa gcaagccctg gaaaccgtgc
     1441 aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa gtcgtggcca
     1501 ttgcaaataa taacggtggc aaacaggctc ttgagacggt tcagagactt ctcccagttc
     1561 tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcg catgacggag
     1621 ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa gcccacggtt
     1681 tgacgcctgc acaagtggtc gccatcgcct cgaatggcgg cggtaagcag gcgctggaaa
     1741 cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca gaccaggtag
     1801 tcgcaatcgc gtcaaacgga gggggaaagc aagccctgga aaccgtgcaa aggttgttgc
     1861 cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt gcatcccacg
     1921 acggtggcaa acaggctctt gagacggttc agagacttct cccagttctc tgtcaagccc
     1981 acgggctgac tcccgatcaa gttgtagcga ttgcgtccaa cggtggaggg aaacaagcat
     2041 tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtctg acacccgaac
     2101 aggtggtcgc cattgcttcc cacgacggag gacggccagc cttggagtcc atcgtagccc
     2161 aattgtccag gcccgatccc gcgttggctg cgttaacgaa tgaccatctg gtggcgttgg
     2221 catgtcttgg tggacgaccc gcgctcgatg cagtcaaaaa gggtctgcct catgctcccg
     2281 cattgatcaa aagaaccaac cggcggattc ccgagagaac ttcccatcga gtcgcgggat
     2341 ccgatccaaa aaagaagaga aaggtagcac ccccgaccga tgtcagcctg ggggacgagc
     2401 tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac gatttcgatc
     2461 tggacatgtt gggggacggg gattccccgg gtccgggatt taccccccac gactccgccc
     2521 cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc gatgcccttg
     2581 gaattgacga gtacggtggg tagtaatcta gg
//