LOCUS       BBa_K782040             3041 bp    DNA     linear       29-Apr-2024
DEFINITION  TALA based fast degradable KRAB (CL1-PEST tag)
ACCESSION   BBa_K782040
VERSION     BBa_K782040.1  
FEATURES             Location/Qualifiers
     misc_feature    1..253
                     /label=CL1PEST
     CDS             254..2619
                     /label=TAL A
     CDS             2625..2648
                     /label=NLS
     CDS             2649..3032
                     /label=KRAB
ORIGIN
        1 agcttgccac catgggccac catcaccacc atcacaattc tgcttgcaag aactggttca
       61 gtagcttaag ccactttgtg atccacctta acagccacgg cttccctccc gaggtggagg
      121 agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg gatagacacc
      181 ctgctgcttg cgccagcgcc aggatcaacg tcgattacaa agaccatgat ggtgattata
      241 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga
      301 aggtgggcat tcaccgcggg gtacctatgg tggacttgag gacactcggt tattcgcaac
      361 agcaacagga gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc
      421 ttgtggggca tggcttcact catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc
      481 ttgggacggt ggctgtcaaa taccaagata tgattgcggc cctgcccgaa gccacgcacg
      541 aggcaattgt aggggtcggt aaacagtggt cgggagcgcg agcacttgag gcgctgctga
      601 ctgtggcggg tgagcttagg gggcctccgc tccagctcga caccgggcag ctgctgaaga
      661 tcgcgaagag agggggagta acagcggtag aggcagtgca cgcctggcgc aatgcgctca
      721 ccggggcccc cttgaacctg accccagacc aggtagtcgc aatcgcgtca aacggagggg
      781 gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa gaccacggcc
      841 ttacaccgga gcaagtcgtg gccattgcaa gcaatggggg tggcaaacag gctcttgaga
      901 cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc gatcaagttg
      961 tagcgattgc gtcgaacatt ggagggaaac aagcattgga gactgtccaa cggctccttc
     1021 ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc gccagccatg
     1081 atggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg tgccaggatc
     1141 atggactgac cccagaccag gtagtcgcaa tcgcgtcaaa cggaggggga aagcaagccc
     1201 tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt acaccggagc
     1261 aagtcgtggc cattgcaaat aataacggtg gcaaacaggc tcttgagacg gttcagagac
     1321 ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta gcgattgcgt
     1381 cgcatgacgg agggaaacaa gcattggaga ctgtccaacg gctccttccc gtgttgtgtc
     1441 aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc ctcgaatggc ggcggtaagc
     1501 aggcgctgga aacagtacag cgcctgctgc ctgtactgtg ccaggatcat ggactgaccc
     1561 cagaccaggt agtcgcaatc gcgaacaata atgggggaaa gcaagccctg gaaaccgtgc
     1621 aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa gtcgtggcca
     1681 ttgcatccca cgacggtggc aaacaggctc ttgagacggt tcagagactt ctcccagttc
     1741 tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcc aacggtggag
     1801 ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa gcccacggtt
     1861 tgacgcctgc acaagtggtc gccatcgcca gccatgatgg cggtaagcag gcgctggaaa
     1921 cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca gaccaggtag
     1981 tcgcaatcgc gtcacatgac gggggaaagc aagccctgga aaccgtgcaa aggttgttgc
     2041 cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt gcatcccacg
     2101 acggtggcaa acaggctctt gagacggttc agagacttct cccagttctc tgtcaagccc
     2161 acgggctgac tcccgatcaa gttgtagcga ttgcgaataa caatggaggg aaacaagcat
     2221 tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg acgcctgcac
     2281 aagtggtcgc catcgccagc catgatggcg gtaagcaggc gctggaaaca gtacagcgcc
     2341 tgctgcctgt actgtgccag gatcatggac tgacacccga acaggtggtc gccattgctt
     2401 ctaatggggg aggacggcca gccttggagt ccatcgtagc ccaattgtcc aggcccgatc
     2461 ccgcgttggc tgcgttaacg aatgaccatc tggtggcgtt ggcatgtctt ggtggacgac
     2521 ccgcgctcga tgcagtcaaa aagggtctgc ctcatgctcc cgcattgatc aaaagaacca
     2581 accggcggat tcccgagaga acttcccatc gagtcgcggg atccgatcca aaaaagaaga
     2641 gaaaggtacc aaaaaagaag agaaaggtcg acggcggtgg tgctttgtct cctcagcact
     2701 ctgctgtcac tcaaggaagt atcatcaaga acaaggaggg catggatgct aagtcactaa
     2761 ctgcctggtc ccggacactg gtgaccttca aggatgtatt tgtggacttc accagggagg
     2821 agtggaagct gctggacact gctcagcaga tcgtgtacag aaatgtgatg ctggagaact
     2881 ataagaacct ggtttccttg ggttatcagc ttactaagcc agatgtgatc ctccggttgg
     2941 agaagggaga agagccctgg ctggtggaga gagaaattca ccaagagacc catcctgatt
     3001 cagagactgc atttgaaatc aaatcatcag tttaatctag g
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