LOCUS       BBa_K782041             2939 bp    DNA     linear       14-Nov-2024
DEFINITION  TALD based fast degradable KRAB (CL1-PEST tag)
ACCESSION   BBa_K782041
VERSION     BBa_K782041.1  
FEATURES             Location/Qualifiers
     misc_feature    1..253
                     /label=CL1PEST
     CDS             254..2517
                     /label=TAL D
     CDS             2523..2546
                     /label=NLS
     CDS             2547..2930
                     /label=KRAB
ORIGIN
        1 agcttgccac catgggccac catcaccacc atcacaattc tgcttgcaag aactggttca
       61 gtagcttaag ccactttgtg atccacctta acagccacgg cttccctccc gaggtggagg
      121 agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg gatagacacc
      181 ctgctgcttg cgccagcgcc aggatcaacg tcgattacaa agaccatgat ggtgattata
      241 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga
      301 aggtgggcat tcaccgcggg gtacctatgg tggacttgag gacactcggt tattcgcaac
      361 agcaacagga gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc
      421 ttgtggggca tggcttcact catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc
      481 ttgggacggt ggctgtcaaa taccaagata tgattgcggc cctgcccgaa gccacgcacg
      541 aggcaattgt aggggtcggt aaacagtggt cgggagcgcg agcacttgag gcgctgctga
      601 ctgtggcggg tgagcttagg gggcctccgc tccagctcga caccgggcag ctgctgaaga
      661 tcgcgaagag agggggagta acagcggtag aggcagtgca cgcctggcgc aatgcgctca
      721 ccggggcccc cttgaacctg accccagacc aggtagtcgc aatcgcgtca catgacgggg
      781 gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa gaccacggcc
      841 ttacaccgga gcaagtcgtg gccattgcaa ataataacgg tggcaaacag gctcttgaga
      901 cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc gatcaagttg
      961 tagcgattgc gtccaacggt ggagggaaac aagcattgga gactgtccaa cggctccttc
     1021 ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc gccagccatg
     1081 atggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg tgccaggatc
     1141 atggactgac cccagaccag gtagtcgcaa tcgcgtcaca tgacggggga aagcaagccc
     1201 tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt acaccggagc
     1261 aagtcgtggc cattgcaagc aacatcggtg gcaaacaggc tcttgagacg gttcagagac
     1321 ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta gcgattgcgt
     1381 cgaacattgg agggaaacaa gcattggaga ctgtccaacg gctccttccc gtgttgtgtc
     1441 aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc ctcgaatggc ggcggtaagc
     1501 aggcgctgga aacagtacag cgcctgctgc ctgtactgtg ccaggatcat ggactgaccc
     1561 cagaccaggt agtcgcaatc gcgtcgaaca ttgggggaaa gcaagccctg gaaaccgtgc
     1621 aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa gtcgtggcca
     1681 ttgcaaataa taacggtggc aaacaggctc ttgagacggt tcagagactt ctcccagttc
     1741 tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcg catgacggag
     1801 ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa gcccacggtt
     1861 tgacgcctgc acaagtggtc gccatcgcct cgaatggcgg cggtaagcag gcgctggaaa
     1921 cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca gaccaggtag
     1981 tcgcaatcgc gtcaaacgga gggggaaagc aagccctgga aaccgtgcaa aggttgttgc
     2041 cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt gcatcccacg
     2101 acggtggcaa acaggctctt gagacggttc agagacttct cccagttctc tgtcaagccc
     2161 acgggctgac tcccgatcaa gttgtagcga ttgcgtccaa cggtggaggg aaacaagcat
     2221 tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtctg acacccgaac
     2281 aggtggtcgc cattgcttcc cacgacggag gacggccagc cttggagtcc atcgtagccc
     2341 aattgtccag gcccgatccc gcgttggctg cgttaacgaa tgaccatctg gtggcgttgg
     2401 catgtcttgg tggacgaccc gcgctcgatg cagtcaaaaa gggtctgcct catgctcccg
     2461 cattgatcaa aagaaccaac cggcggattc ccgagagaac ttcccatcga gtcgcgggat
     2521 ccgatccaaa aaagaagaga aaggtaccaa aaaagaagag aaaggtcgac ggcggtggtg
     2581 ctttgtctcc tcagcactct gctgtcactc aaggaagtat catcaagaac aaggagggca
     2641 tggatgctaa gtcactaact gcctggtccc ggacactggt gaccttcaag gatgtatttg
     2701 tggacttcac cagggaggag tggaagctgc tggacactgc tcagcagatc gtgtacagaa
     2761 atgtgatgct ggagaactat aagaacctgg tttccttggg ttatcagctt actaagccag
     2821 atgtgatcct ccggttggag aagggagaag agccctggct ggtggagaga gaaattcacc
     2881 aagagaccca tcctgattca gagactgcat ttgaaatcaa atcatcagtt taatctagg
//