LOCUS BBa_K782042 3041 bp DNA linear 14-Nov-2024 DEFINITION TALB based fast degradable KRAB (CL1-PEST tag) ACCESSION BBa_K782042 VERSION BBa_K782042.1 FEATURES Location/Qualifiers misc_feature 1..253 /label=CL1PEST CDS 254..2619 /label=TAL B CDS 2625..2648 /label=NLS CDS 2649..3032 /label=KRAB ORIGIN 1 agcttgccac catgggccac catcaccacc atcacaattc tgcttgcaag aactggttca 61 gtagcttaag ccactttgtg atccacctta acagccacgg cttccctccc gaggtggagg 121 agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg gatagacacc 181 ctgctgcttg cgccagcgcc aggatcaacg tcgattacaa agaccatgat ggtgattata 241 aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag aagaagagga 301 aggtgggcat tcaccgcggg gtacctatgg tggacttgag gacactcggt tattcgcaac 361 agcaacagga gaaaatcaag cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc 421 ttgtggggca tggcttcact catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc 481 ttgggacggt ggctgtcaaa taccaagata tgattgcggc cctgcccgaa gccacgcacg 541 aggcaattgt aggggtcggt aaacagtggt cgggagcgcg agcacttgag gcgctgctga 601 ctgtggcggg tgagcttagg gggcctccgc tccagctcga caccgggcag ctgctgaaga 661 tcgcgaagag agggggagta acagcggtag aggcagtgca cgcctggcgc aatgcgctca 721 ccggggcccc cttgaacctg accccagacc aggtagtcgc aatcgcgtca catgacgggg 781 gaaagcaagc cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa gaccacggcc 841 ttacaccgga gcaagtcgtg gccattgcaa gcaatggggg tggcaaacag gctcttgaga 901 cggttcagag acttctccca gttctctgtc aagcccacgg gctgactccc gatcaagttg 961 tagcgattgc gtccaacggt ggagggaaac aagcattgga gactgtccaa cggctccttc 1021 ccgtgttgtg tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc gccagccatg 1081 atggcggtaa gcaggcgctg gaaacagtac agcgcctgct gcctgtactg tgccaggatc 1141 atggactgac cccagaccag gtagtcgcaa tcgcgtcaca tgacggggga aagcaagccc 1201 tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt acaccggagc 1261 aagtcgtggc cattgcaaat aataacggtg gcaaacaggc tcttgagacg gttcagagac 1321 ttctcccagt tctctgtcaa gcccacgggc tgactcccga tcaagttgta gcgattgcgt 1381 ccaacggtgg agggaaacaa gcattggaga ctgtccaacg gctccttccc gtgttgtgtc 1441 aagcccacgg tttgacgcct gcacaagtgg tcgccatcgc ctcgaatggc ggcggtaagc 1501 aggcgctgga aacagtacag cgcctgctgc ctgtactgtg ccaggatcat ggactgaccc 1561 cagaccaggt agtcgcaatc gcgtcaaacg gagggggaaa gcaagccctg gaaaccgtgc 1621 aaaggttgtt gccggtcctt tgtcaagacc acggccttac accggagcaa gtcgtggcca 1681 ttgcatccca cgacggtggc aaacaggctc ttgagacggt tcagagactt ctcccagttc 1741 tctgtcaagc ccacgggctg actcccgatc aagttgtagc gattgcgtcg catgacggag 1801 ggaaacaagc attggagact gtccaacggc tccttcccgt gttgtgtcaa gcccacggtt 1861 tgacgcctgc acaagtggtc gccatcgcct ccaatattgg cggtaagcag gcgctggaaa 1921 cagtacagcg cctgctgcct gtactgtgcc aggatcatgg actgacccca gaccaggtag 1981 tcgcaatcgc gtcacatgac gggggaaagc aagccctgga aaccgtgcaa aggttgttgc 2041 cggtcctttg tcaagaccac ggccttacac cggagcaagt cgtggccatt gcaagcaaca 2101 tcggtggcaa acaggctctt gagacggttc agagacttct cccagttctc tgtcaagccc 2161 acgggctgac tcccgatcaa gttgtagcga ttgcgtccaa cggtggaggg aaacaagcat 2221 tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg acgcctgcac 2281 aagtggtcgc catcgccagc catgatggcg gtaagcaggc gctggaaaca gtacagcgcc 2341 tgctgcctgt actgtgccag gatcatggac tgacacccga acaggtggtc gccattgctt 2401 ctaatggggg aggacggcca gccttggagt ccatcgtagc ccaattgtcc aggcccgatc 2461 ccgcgttggc tgcgttaacg aatgaccatc tggtggcgtt ggcatgtctt ggtggacgac 2521 ccgcgctcga tgcagtcaaa aagggtctgc ctcatgctcc cgcattgatc aaaagaacca 2581 accggcggat tcccgagaga acttcccatc gagtcgcggg atccgatcca aaaaagaaga 2641 gaaaggtacc aaaaaagaag agaaaggtcg acggcggtgg tgctttgtct cctcagcact 2701 ctgctgtcac tcaaggaagt atcatcaaga acaaggaggg catggatgct aagtcactaa 2761 ctgcctggtc ccggacactg gtgaccttca aggatgtatt tgtggacttc accagggagg 2821 agtggaagct gctggacact gctcagcaga tcgtgtacag aaatgtgatg ctggagaact 2881 ataagaacct ggtttccttg ggttatcagc ttactaagcc agatgtgatc ctccggttgg 2941 agaagggaga agagccctgg ctggtggaga gagaaattca ccaagagacc catcctgatt 3001 cagagactgc atttgaaatc aaatcatcag tttaatctag g //