LOCUS BBa_K782083 4975 bp DNA linear 29-Nov-2024 DEFINITION 10x[TALB] operator_CMV promoter_TALA:KRAB:NLS_t2a_mCitrine ACCESSION BBa_K782083 VERSION BBa_K782083.1 FEATURES Location/Qualifiers misc_feature 31..693 /label=10x[C+B] binding sites promoter 750..1333 /label=CMV CDS 1367..3778 /label=TAL A CDS 3779..3802 /label=NLS CDS 3803..4186 /label=KRAB misc_feature 4187..4246 /label=t2a CDS 4247..4966 /label=Citrine ORIGIN 1 aattcggtgg caccggtttg attgcacagc taaagagcag aaactcatct tccgtttcca 61 catcttgctg gcagccgggg taccgcaggc aagtcgctaa agagcagaaa ctcatcttcc 121 gtttccacat ctatggaatc acggcggccg gccgtatctc atcctaaaga gcagaaactc 181 atcttccgtt tccacatcta atgcgtcggc gtgcggccgg tgccagttgc ataaagagca 241 gaaactcatc ttccgtttcc acatctttgg ctgtcctagg gtacctggac gctccttgta 301 aagagcagaa actcatcttc cgtttccaca tcttggctgg tgccgcaccg gtaagcagaa 361 ctagaggtgg caccggtttg attgcacagc taaagagcag aaactcatct tccgtttcca 421 catcttgctg gcagccgggg taccgcaggc aagtcgctaa agagcagaaa ctcatcttcc 481 gtttccacat ctatggaatc acggcggccg gccgtatctc atcctaaaga gcagaaactc 541 atcttccgtt tccacatcta atgcgtcggc gtgcggccgg tgccagttgc ataaagagca 601 gaaactcatc ttccgtttcc acatctttgg ctgtcctagg gtacctggac gctccttgta 661 aagagcagaa actcatcttc cgtttccaca tcttggctgg tgccgcaccg gtaagcagag 721 gatcctgtac gggccagata tacgcgttga cattgattat tgtctagtta ttaatagtaa 781 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 841 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 901 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta 961 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 1021 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 1081 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 1141 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 1201 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 1261 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 1321 ataagcagag ctcaagcttg ccaccatgca ccaccaccac caccacgact acaaagacca 1381 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agatggcccc 1441 caagaagaag aggaaggtgg gcattcaccg cggggtacct atggtggact tgaggacact 1501 cggttattcg caacagcaac aggagaaaat caagcctaag gtcaggagca ccgtcgcgca 1561 acaccacgag gcgcttgtgg ggcatggctt cactcatgcg catattgtcg cgctttcaca 1621 gcaccctgcg gcgcttggga cggtggctgt caaataccaa gatatgattg cggccctgcc 1681 cgaagccacg cacgaggcaa ttgtaggggt cggtaaacag tggtcgggag cgcgagcact 1741 tgaggcgctg ctgactgtgg cgggtgagct tagggggcct ccgctccagc tcgacaccgg 1801 gcagctgctg aagatcgcga agagaggggg agtaacagcg gtagaggcag tgcacgcctg 1861 gcgcaatgcg ctcaccgggg cccccttgaa cctgacccca gaccaggtag tcgcaatcgc 1921 gtcaaacgga gggggaaagc aagccctgga aaccgtgcaa aggttgttgc cggtcctttg 1981 tcaagaccac ggccttacac cggagcaagt cgtggccatt gcaagcaatg ggggtggcaa 2041 acaggctctt gagacggttc agagacttct cccagttctc tgtcaagccc acgggctgac 2101 tcccgatcaa gttgtagcga ttgcgtcgaa cattggaggg aaacaagcat tggagactgt 2161 ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg acgcctgcac aagtggtcgc 2221 catcgccagc catgatggcg gtaagcaggc gctggaaaca gtacagcgcc tgctgcctgt 2281 actgtgccag gatcatggac tgaccccaga ccaggtagtc gcaatcgcgt caaacggagg 2341 gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg gtcctttgtc aagaccacgg 2401 ccttacaccg gagcaagtcg tggccattgc aaataataac ggtggcaaac aggctcttga 2461 gacggttcag agacttctcc cagttctctg tcaagcccac gggctgactc ccgatcaagt 2521 tgtagcgatt gcgtcgcatg acggagggaa acaagcattg gagactgtcc aacggctcct 2581 tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa gtggtcgcca tcgcctcgaa 2641 tggcggcggt aagcaggcgc tggaaacagt acagcgcctg ctgcctgtac tgtgccagga 2701 tcatggactg accccagacc aggtagtcgc aatcgcgaac aataatgggg gaaagcaagc 2761 cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa gaccacggcc ttacaccgga 2821 gcaagtcgtg gccattgcat cccacgacgg tggcaaacag gctcttgaga cggttcagag 2881 acttctccca gttctctgtc aagcccacgg gctgactccc gatcaagttg tagcgattgc 2941 gtccaacggt ggagggaaac aagcattgga gactgtccaa cggctccttc ccgtgttgtg 3001 tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc gccagccatg atggcggtaa 3061 gcaggcgctg gaaacagtac agcgcctgct gcctgtactg tgccaggatc atggactgac 3121 cccagaccag gtagtcgcaa tcgcgtcaca tgacggggga aagcaagccc tggaaaccgt 3181 gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt acaccggagc aagtcgtggc 3241 cattgcatcc cacgacggtg gcaaacaggc tcttgagacg gttcagagac ttctcccagt 3301 tctctgtcaa gcccacgggc tgactcccga tcaagttgta gcgattgcga ataacaatgg 3361 agggaaacaa gcattggaga ctgtccaacg gctccttccc gtgttgtgtc aagcccacgg 3421 tttgacgcct gcacaagtgg tcgccatcgc cagccatgat ggcggtaagc aggcgctgga 3481 aacagtacag cgcctgctgc ctgtactgtg ccaggatcat ggactgacac ccgaacaggt 3541 ggtcgccatt gcttctaatg ggggaggacg gccagccttg gagtccatcg tagcccaatt 3601 gtccaggccc gatcccgcgt tggctgcgtt aacgaatgac catctggtgg cgttggcatg 3661 tcttggtgga cgacccgcgc tcgatgcagt caaaaagggt ctgcctcatg ctcccgcatt 3721 gatcaaaaga accaaccggc ggattcccga gagaacttcc catcgagtcg cgggatccga 3781 tccaaaaaag aagagaaagg taccaaaaaa gaagagaaag gtcgacggcg gtggtgcttt 3841 gtctcctcag cactctgctg tcactcaagg aagtatcatc aagaacaagg agggcatgga 3901 tgctaagtca ctaactgcct ggtcccggac actggtgacc ttcaaggatg tatttgtgga 3961 cttcaccagg gaggagtgga agctgctgga cactgctcag cagatcgtgt acagaaatgt 4021 gatgctggag aactataaga acctggtttc cttgggttat cagcttacta agccagatgt 4081 gatcctccgg ttggagaagg gagaagagcc ctggctggtg gagagagaaa ttcaccaaga 4141 gacccatcct gattcagaga ctgcatttga aatcaaatca tcagttagag ccgaggggag 4201 aggaagtctt ctgacctgcg gagacgtcga agagaatcct ggacccatgg tgagcaaggg 4261 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 4321 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 4381 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt 4441 cggctacggc ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt 4501 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 4561 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 4621 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 4681 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 4741 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 4801 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca 4861 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 4921 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat ctagg //