LOCUS BBa_K782084 4984 bp DNA linear 26-Nov-2024 DEFINITION 10x[TALA] operator_CMV promoter_TALB:NLS:KRAB_t2a_mNeptune ACCESSION BBa_K782084 VERSION BBa_K782084.1 FEATURES Location/Qualifiers misc_feature 31..693 /label=10x[A+C] binding sites promoter 750..1333 /label=CMV CDS 1367..3778 /label=TAL B CDS 3779..3802 /label=NLS CDS 3803..4186 /label=KRAB misc_feature 4187..4246 /label=t2a CDS 4247..4975 /label=Neptune ORIGIN 1 aattcggtgg caccggtttg attgcacagc tttactgctg ctcccgctta aagagcagaa 61 actcatgctg gcagccgggg taccgcaggc aagtcgcttt actgctgctc ccgcttaaag 121 agcagaaact caatggaatc acggcggccg gccgtatctc atcctttact gctgctcccg 181 cttaaagagc agaaactcaa atgcgtcggc gtgcggccgg tgccagttgc atttactgct 241 gctcccgctt aaagagcaga aactcattgg ctgtcctagg gtacctggac gctccttgtt 301 tactgctgct cccgcttaaa gagcagaaac tcatggctgg tgccgcaccg gtaagcagaa 361 ctagaggtgg caccggtttg attgcacagc tttactgctg ctcccgctta aagagcagaa 421 actcatgctg gcagccgggg taccgcaggc aagtcgcttt actgctgctc ccgcttaaag 481 agcagaaact caatggaatc acggcggccg gccgtatctc atcctttact gctgctcccg 541 cttaaagagc agaaactcaa atgcgtcggc gtgcggccgg tgccagttgc atttactgct 601 gctcccgctt aaagagcaga aactcattgg ctgtcctagg gtacctggac gctccttgtt 661 tactgctgct cccgcttaaa gagcagaaac tcatggctgg tgccgcaccg gtaagcagag 721 gatcctgtac gggccagata tacgcgttga cattgattat tgtctagtta ttaatagtaa 781 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 841 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 901 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggactattta 961 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 1021 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 1081 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 1141 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 1201 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 1261 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 1321 ataagcagag ctcaagcttg ccaccatgca ccaccaccac caccacgact acaaagacca 1381 tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca agatggcccc 1441 caagaagaag aggaaggtgg gcattcaccg cggggtacct atggtggact tgaggacact 1501 cggttattcg caacagcaac aggagaaaat caagcctaag gtcaggagca ccgtcgcgca 1561 acaccacgag gcgcttgtgg ggcatggctt cactcatgcg catattgtcg cgctttcaca 1621 gcaccctgcg gcgcttggga cggtggctgt caaataccaa gatatgattg cggccctgcc 1681 cgaagccacg cacgaggcaa ttgtaggggt cggtaaacag tggtcgggag cgcgagcact 1741 tgaggcgctg ctgactgtgg cgggtgagct tagggggcct ccgctccagc tcgacaccgg 1801 gcagctgctg aagatcgcga agagaggggg agtaacagcg gtagaggcag tgcacgcctg 1861 gcgcaatgcg ctcaccgggg cccccttgaa cctgacccca gaccaggtag tcgcaatcgc 1921 gtcacatgac gggggaaagc aagccctgga aaccgtgcaa aggttgttgc cggtcctttg 1981 tcaagaccac ggccttacac cggagcaagt cgtggccatt gcaagcaatg ggggtggcaa 2041 acaggctctt gagacggttc agagacttct cccagttctc tgtcaagccc acgggctgac 2101 tcccgatcaa gttgtagcga ttgcgtccaa cggtggaggg aaacaagcat tggagactgt 2161 ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg acgcctgcac aagtggtcgc 2221 catcgccagc catgatggcg gtaagcaggc gctggaaaca gtacagcgcc tgctgcctgt 2281 actgtgccag gatcatggac tgaccccaga ccaggtagtc gcaatcgcgt cacatgacgg 2341 gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg gtcctttgtc aagaccacgg 2401 ccttacaccg gagcaagtcg tggccattgc aaataataac ggtggcaaac aggctcttga 2461 gacggttcag agacttctcc cagttctctg tcaagcccac gggctgactc ccgatcaagt 2521 tgtagcgatt gcgtccaacg gtggagggaa acaagcattg gagactgtcc aacggctcct 2581 tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa gtggtcgcca tcgcctcgaa 2641 tggcggcggt aagcaggcgc tggaaacagt acagcgcctg ctgcctgtac tgtgccagga 2701 tcatggactg accccagacc aggtagtcgc aatcgcgtca aacggagggg gaaagcaagc 2761 cctggaaacc gtgcaaaggt tgttgccggt cctttgtcaa gaccacggcc ttacaccgga 2821 gcaagtcgtg gccattgcat cccacgacgg tggcaaacag gctcttgaga cggttcagag 2881 acttctccca gttctctgtc aagcccacgg gctgactccc gatcaagttg tagcgattgc 2941 gtcgcatgac ggagggaaac aagcattgga gactgtccaa cggctccttc ccgtgttgtg 3001 tcaagcccac ggtttgacgc ctgcacaagt ggtcgccatc gcctccaata ttggcggtaa 3061 gcaggcgctg gaaacagtac agcgcctgct gcctgtactg tgccaggatc atggactgac 3121 cccagaccag gtagtcgcaa tcgcgtcaca tgacggggga aagcaagccc tggaaaccgt 3181 gcaaaggttg ttgccggtcc tttgtcaaga ccacggcctt acaccggagc aagtcgtggc 3241 cattgcaagc aacatcggtg gcaaacaggc tcttgagacg gttcagagac ttctcccagt 3301 tctctgtcaa gcccacgggc tgactcccga tcaagttgta gcgattgcgt ccaacggtgg 3361 agggaaacaa gcattggaga ctgtccaacg gctccttccc gtgttgtgtc aagcccacgg 3421 tttgacgcct gcacaagtgg tcgccatcgc cagccatgat ggcggtaagc aggcgctgga 3481 aacagtacag cgcctgctgc ctgtactgtg ccaggatcat ggactgacac ccgaacaggt 3541 ggtcgccatt gcttctaatg ggggaggacg gccagccttg gagtccatcg tagcccaatt 3601 gtccaggccc gatcccgcgt tggctgcgtt aacgaatgac catctggtgg cgttggcatg 3661 tcttggtgga cgacccgcgc tcgatgcagt caaaaagggt ctgcctcatg ctcccgcatt 3721 gatcaaaaga accaaccggc ggattcccga gagaacttcc catcgagtcg cgggatccga 3781 tccaaaaaag aagagaaagg taccaaaaaa gaagagaaag gtcgacggcg gtggtgcttt 3841 gtctcctcag cactctgctg tcactcaagg aagtatcatc aagaacaagg agggcatgga 3901 tgctaagtca ctaactgcct ggtcccggac actggtgacc ttcaaggatg tatttgtgga 3961 cttcaccagg gaggagtgga agctgctgga cactgctcag cagatcgtgt acagaaatgt 4021 gatgctggag aactataaga acctggtttc cttgggttat cagcttacta agccagatgt 4081 gatcctccgg ttggagaagg gagaagagcc ctggctggtg gagagagaaa ttcaccaaga 4141 gacccatcct gattcagaga ctgcatttga aatcaaatca tcagttagag ccgaggggag 4201 aggaagtctt ctgacctgcg gagacgtcga agagaatcct ggacccgtgt ctaagggcga 4261 agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca 4321 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccggcag 4381 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctacctgctt 4441 catgtacggc agcaagacct tcatcaacca cacccagggc atccccgatt tctttaagca 4501 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct 4561 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag 4621 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc 4681 cagcaccgag accctgtacc ccgctgacgg cggcctggaa ggcagatgcg acatggccct 4741 gaagctcgtg ggcgggggcc acctgatctg caacctgaag accacataca gatccaagaa 4801 acccgctaag aacctcaaga tgcccggcgt ctactttgtg gaccgcagac tggaaagaat 4861 caaggaggcc gacaatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg 4921 cgacctccct agcaaactgg ggcacaaact taatggcatg gacgagctgt acaagtaatc 4981 tagg //