LOCUS BBa_K782085 4293 bp DNA linear 26-Nov-2024 DEFINITION 10x[TALA] operator_minimal promoter_TALA:NLS:VP16_t2a_BFP ACCESSION BBa_K782085 VERSION BBa_K782085.1 FEATURES Location/Qualifiers misc_feature 31..693 /label=10x[A+C] binding sites promoter 756..787 /label=Minimal promoter CDS 856..3267 /label=TAL A CDS 3268..3291 /label=NLS CDS 3292..3525 /label=VP:16 misc_feature 3526..3585 /label=t2a CDS 3586..4284 /label=BFP ORIGIN 1 aattcggtgg caccggtttg attgcacagc tttactgctg ctcccgctta aagagcagaa 61 actcatgctg gcagccgggg taccgcaggc aagtcgcttt actgctgctc ccgcttaaag 121 agcagaaact caatggaatc acggcggccg gccgtatctc atcctttact gctgctcccg 181 cttaaagagc agaaactcaa atgcgtcggc gtgcggccgg tgccagttgc atttactgct 241 gctcccgctt aaagagcaga aactcattgg ctgtcctagg gtacctggac gctccttgtt 301 tactgctgct cccgcttaaa gagcagaaac tcatggctgg tgccgcaccg gtaagcagaa 361 ctagaggtgg caccggtttg attgcacagc tttactgctg ctcccgctta aagagcagaa 421 actcatgctg gcagccgggg taccgcaggc aagtcgcttt actgctgctc ccgcttaaag 481 agcagaaact caatggaatc acggcggccg gccgtatctc atcctttact gctgctcccg 541 cttaaagagc agaaactcaa atgcgtcggc gtgcggccgg tgccagttgc atttactgct 601 gctcccgctt aaagagcaga aactcattgg ctgtcctagg gtacctggac gctccttgtt 661 tactgctgct cccgcttaaa gagcagaaac tcatggctgg tgccgcaccg gtaagcagag 721 gatcctgtac gggccagata tacgcgttga gatcttagag ggtatataat ggaagctcga 781 cttccagctc gagggcaatc cggtactgtt ggtaaagcca cctaattcgc caccatgcac 841 caccaccacc accacgacta caaagaccat gacggtgatt ataaagatca tgacatcgat 901 tacaaggatg acgatgacaa gatggccccc aagaagaaga ggaaggtggg cattcaccgc 961 ggggtaccta tggtggactt gaggacactc ggttattcgc aacagcaaca ggagaaaatc 1021 aagcctaagg tcaggagcac cgtcgcgcaa caccacgagg cgcttgtggg gcatggcttc 1081 actcatgcgc atattgtcgc gctttcacag caccctgcgg cgcttgggac ggtggctgtc 1141 aaataccaag atatgattgc ggccctgccc gaagccacgc acgaggcaat tgtaggggtc 1201 ggtaaacagt ggtcgggagc gcgagcactt gaggcgctgc tgactgtggc gggtgagctt 1261 agggggcctc cgctccagct cgacaccggg cagctgctga agatcgcgaa gagaggggga 1321 gtaacagcgg tagaggcagt gcacgcctgg cgcaatgcgc tcaccggggc ccccttgaac 1381 ctgaccccag accaggtagt cgcaatcgcg tcaaacggag ggggaaagca agccctggaa 1441 accgtgcaaa ggttgttgcc ggtcctttgt caagaccacg gccttacacc ggagcaagtc 1501 gtggccattg caagcaatgg gggtggcaaa caggctcttg agacggttca gagacttctc 1561 ccagttctct gtcaagccca cgggctgact cccgatcaag ttgtagcgat tgcgtcgaac 1621 attggaggga aacaagcatt ggagactgtc caacggctcc ttcccgtgtt gtgtcaagcc 1681 cacggtttga cgcctgcaca agtggtcgcc atcgccagcc atgatggcgg taagcaggcg 1741 ctggaaacag tacagcgcct gctgcctgta ctgtgccagg atcatggact gaccccagac 1801 caggtagtcg caatcgcgtc aaacggaggg ggaaagcaag ccctggaaac cgtgcaaagg 1861 ttgttgccgg tcctttgtca agaccacggc cttacaccgg agcaagtcgt ggccattgca 1921 aataataacg gtggcaaaca ggctcttgag acggttcaga gacttctccc agttctctgt 1981 caagcccacg ggctgactcc cgatcaagtt gtagcgattg cgtcgcatga cggagggaaa 2041 caagcattgg agactgtcca acggctcctt cccgtgttgt gtcaagccca cggtttgacg 2101 cctgcacaag tggtcgccat cgcctcgaat ggcggcggta agcaggcgct ggaaacagta 2161 cagcgcctgc tgcctgtact gtgccaggat catggactga ccccagacca ggtagtcgca 2221 atcgcgaaca ataatggggg aaagcaagcc ctggaaaccg tgcaaaggtt gttgccggtc 2281 ctttgtcaag accacggcct tacaccggag caagtcgtgg ccattgcatc ccacgacggt 2341 ggcaaacagg ctcttgagac ggttcagaga cttctcccag ttctctgtca agcccacggg 2401 ctgactcccg atcaagttgt agcgattgcg tccaacggtg gagggaaaca agcattggag 2461 actgtccaac ggctccttcc cgtgttgtgt caagcccacg gtttgacgcc tgcacaagtg 2521 gtcgccatcg ccagccatga tggcggtaag caggcgctgg aaacagtaca gcgcctgctg 2581 cctgtactgt gccaggatca tggactgacc ccagaccagg tagtcgcaat cgcgtcacat 2641 gacgggggaa agcaagccct ggaaaccgtg caaaggttgt tgccggtcct ttgtcaagac 2701 cacggcctta caccggagca agtcgtggcc attgcatccc acgacggtgg caaacaggct 2761 cttgagacgg ttcagagact tctcccagtt ctctgtcaag cccacgggct gactcccgat 2821 caagttgtag cgattgcgaa taacaatgga gggaaacaag cattggagac tgtccaacgg 2881 ctccttcccg tgttgtgtca agcccacggt ttgacgcctg cacaagtggt cgccatcgcc 2941 agccatgatg gcggtaagca ggcgctggaa acagtacagc gcctgctgcc tgtactgtgc 3001 caggatcatg gactgacacc cgaacaggtg gtcgccattg cttctaatgg gggaggacgg 3061 ccagccttgg agtccatcgt agcccaattg tccaggcccg atcccgcgtt ggctgcgtta 3121 acgaatgacc atctggtggc gttggcatgt cttggtggac gacccgcgct cgatgcagtc 3181 aaaaagggtc tgcctcatgc tcccgcattg atcaaaagaa ccaaccggcg gattcccgag 3241 agaacttccc atcgagtcgc gggatccgat ccaaaaaaga agagaaaggt agcccccccg 3301 accgatgtca gcctggggga cgagctccac ttagacggcg aggacgtggc gatggcgcat 3361 gccgacgcgc tagacgattt cgatctggac atgttggggg acggggattc cccgggtccg 3421 ggatttaccc cccacgactc cgccccctac ggcgctctgg atatggccga cttcgagttt 3481 gagcagatgt ttaccgatgc ccttggaatt gacgagtacg gtgggagagc cgaggggaga 3541 ggaagtcttc tgacctgcgg agacgtcgaa gagaatcctg gacccatgag cgagctgatt 3601 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag 3661 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg 3721 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc 3781 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct 3841 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc 3901 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac 3961 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag 4021 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg 4081 ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag 4141 aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc 4201 aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct 4261 agcaaactgg ggcacaagct caattaatct agg //