BBa_K783062 1 BBa_K783062 Level 1 MoClo Destination Vector with AE 2012-10-01T11:00:00Z 2015-05-08T01:13:21Z We amplified the alpha lacZ fragment from pUC19 and used the BioBrick pSB1C3 backbone for our vector backbone. This is a Level 1 MoClo destination vector with flanking sites A on the 5' side and site E on the 3' side of the alpha fragment of lacZ. The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA. false false _1038_ 0 8248 9 No part sequence false We had to confirm the lacZ orientation was 5'->3' after cloning. false Traci Haddock igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z