##gff-version 3
##sequence-region BBa_K783067 1 3778
BBa_K783067	.	ribosome_entry_site	1261	1273	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K783067	.	ribosome_entry_site	1267	1270	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K783067	.	ribosome_entry_site	1261	1273	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K783067	.	engineered_region	1261	1273	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K783067	.	terminator	3650	3778	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K783067	.	terminator	3738	3778	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K783067	.	engineered_region	3738	3778	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K783067	.	stop_codon	3771	3771	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K783067	.	stem_loop	3745	3764	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K783067	.	polyA_site	3765	3778	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K783067	.	terminator	3650	3729	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K783067	.	engineered_region	3650	3729	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K783067	.	stem_loop	3661	3704	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K783067	.	CDS	1280	1964	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K783067	.	engineered_tag	1940	1964	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K783067	.	tag	1900	1933	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K783067	.	CDS	1283	1899	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K783067	.	terminator	2719	2847	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K783067	.	terminator	2807	2847	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K783067	.	engineered_region	2807	2847	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K783067	.	stop_codon	2840	2840	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K783067	.	stem_loop	2814	2833	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K783067	.	polyA_site	2834	2847	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K783067	.	terminator	2719	2798	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K783067	.	engineered_region	2719	2798	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K783067	.	stem_loop	2730	2773	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K783067	.	engineered_region	1	1114	.	+	0	ID=BBa_I13458;Name=BBa_I13458
BBa_K783067	.	CDS	2936	3641	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K783067	.	engineered_tag	3617	3641	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K783067	.	CDS	2936	3610	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K783067	.	CDS	1991	2710	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K783067	.	CDS	1991	2710	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K783067	.	promoter	1123	1252	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_K783067	.	ribosome_entry_site	2918	2929	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K783067	.	conserved_region	2922	2925	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K783067	.	ribosome_entry_site	1973	1984	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K783067	.	conserved_region	1977	1980	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K783067	.	promoter	2856	2909	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K783067	.	promoter	2875	2880	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K783067	.	non_covalent_binding_site	2881	2899	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K783067	.	engineered_region	2856	2909	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K783067	.	promoter	2898	2903	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K783067	.	non_covalent_binding_site	2856	2874	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K783067
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattag
cggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtcac
acaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaa
tcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataa
gcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagc
tggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatt
taggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcact
agagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtc
gctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcacc
aaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaa
gaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatg
ttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttg
cactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatac
ccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttca
aagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaagg
tattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatg
gcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcag
accattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgc
cctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggc
atggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagag
gagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgtta
acggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttac
caaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacac
ccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacg
gtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaa
cttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagac
ggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacct
acatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaaga
ctacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgt
agatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata