LOCUS       BBa_K784000             1569 bp    DNA     linear       22-Dec-2024
DEFINITION  pT7+RBS+Alkaline Phosphatase (pHO)+Terminator
ACCESSION   BBa_K784000
VERSION     BBa_K784000.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    24..29
                     /label=XhoI restriction site
     CDS             42..1516
                     /label=phoA BioBrick J61032
     RBS             30..41
                     /label=RBS BioBrick B0064
     misc_feature    1517..1522
                     /label=XmaI restriction site
     misc_feature    24..1522
                     /label=BBa_K784027
     terminator      1523..1569
                     /label=BBa_K784002
ORIGIN
        1 taatacgact cactataggg agactcgaga aagaggagaa aatgtttttt attttaactg
       61 tatttataca tggagaaaat aaagtgaaac aaagcgctat gatttttgcc ttgctgcctt
      121 tgctgttggc tccggccagt caggcggaca ccacaacaat atccgtgctg gataatcgtg
      181 cggcgcaggg cgatattact acgcccggcg gcgcgcgccg attaaccggc gatcaaacgg
      241 cggcgctgcg tgaatcgcta aatgataaac cggcgaaaaa tattattctg ctgatcggcg
      301 acggaatggg agattctgaa ataaccgccg cacgaaatta tgccgaaggc gccggcggct
      361 tttttaaagg tattgatgcc ctgccgctga ccggacaata tacccactat gcgctgaata
      421 aaaaaaccgg caaaccagat tacgtgaccg attccgccgc ctcagccacc gcgtggtcca
      481 caggggtgaa aacctacaac ggcgcgctgg gggtcgatat tcatgaaaaa gatcatctga
      541 ccattctcga aatggcgaag gccgcaggtc tggctaccgg caacgtctcg accgcggagt
      601 tgcaggacgc caccccggca gcgctgattg cccatgtcac ctcgcgcaaa tgctatggcc
      661 cgacggcgac cagcgaaaaa tgtccgacaa acgcgctgga aaagggcggc aaaggctcga
      721 ttactgaaca gctcctgaac gcgcgggcgg acgtcaccct gggcggcggc gcgaaaacct
      781 tcgccgagac ggcaaccgcc ggcgagtggc aggggaaaac gctgcgtgag caggcgcaga
      841 tgcgcggcta ccagttagtc ggcgatgccg cctctttagc ggcgatcgac gaagcgaatc
      901 aggacaaacc gctgctggga ctgttcgcag aggggaatat gccggtgcgc tgggaagggc
      961 cgaaggcctc gtaccacggc aatattgata aacctgccgt gacctgtacg ccaaatccga
     1021 aacgcaatga cgcgattccc acgctggcgc agatgaccga caaagccatc gaactgttga
     1081 gcaaaaatga gcggggcttc tttttacagg tggaaggcgc gtctatcgat aagcaggatc
     1141 acgccgcgaa cccgtgcgga cagattggcg agacggtgga tcttgatgaa gccgtacaga
     1201 gggcgctggc ctttgcgaag aaagacggca atacgctggt gatcgttacc gccgatcatg
     1261 ctcatgccag ccagatcgtg gcgcccgaaa cgaaagcgcc ggggctgacg caggcgctga
     1321 acaccaaaga tggcgcggtg atggtcatca gctacggcaa ctcggaagaa gattcccagg
     1381 agcataccgg cagccagctg cgcatcgccg cttacgggcc gcatgcggcg aacgtggtcg
     1441 ggctgaccga tcaaaccgat ctgttctaca ccatgaaagc ggcgctgggc ctgaagtaat
     1501 gccgcctctg ggaattcccg ggtagcataa ccccttgggg cctctaaacg ggtcttgagg
     1561 ggttttttg
//