BBa_K784005
1
BBa_K784005
Theophylline riboswitch 12.1
2012-09-12T11:00:00Z
2015-05-08T01:13:21Z
The part was obtained from the <a href="http://www.gallivanlab.org/">Gallivan lab</a>. The description of the part was found in the following paper:
Lynch Sean A., Gallivan Justin P. 2009. A flow cytometry-based screen for synthetic riboswitches. Nucleic Acids Research 37(1): 184-192.
A riboswitch which is comprised of an aptamer which recognizes the ligand, theophylline. The riboswitch also contains an RBS. In the absence of theophylline, there is extensive base pairing in the 5' untranslated region (UTR) which blocks the RBS. This base pairing prevents translation of a gene downstream to the riboswitch. In the presence of theophylline, the transcript adopts a secondary structure in which the RBS is exposed, allowing translation to occur.
false
false
_1039_
0
11677
9
Not in stock
false
The part must be directly fused to the starting codon of the downstream coding region.
false
Ilya Vainberg Slutskin
annotation2182923
1
Theophylline aptamer
range2182923
1
18
55
annotation2182924
1
RBS
range2182924
1
56
74
annotation2182922
1
Additional 5' UTR
range2182922
1
1
17
BBa_K784005_sequence
1
gactcactataggtaccggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z