LOCUS       BBa_K784010             1479 bp    DNA     linear       22-Dec-2024
DEFINITION  MCS+tac promoter+theophylline riboswitch+mCherry
ACCESSION   BBa_K784010
VERSION     BBa_K784010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..32
                     /label=Spacer2
     RBS             33..51
                     /label=RBS
     misc_feature    17..24
                     /label=PacI restriction site
     RBS             1..51
                     /label=BBa_K784012
     CDS             52..762
                     /label=mCherry
     misc_feature    403..403
                     /label=C->T (removing PstI site)
     misc_feature    52..765
                     /label=BBa_J06504
     misc_feature    1..765
                     /label=BBa_K784009
     CDS             766..1476
                     /label=mCherry
     misc_feature    1117..1117
                     /label=C->T (removing PstI site)
     misc_feature    766..1479
                     /label=BBa_J06504
ORIGIN
        1 tctctctctc tttagattaa ttaatctctc tcctgctaag gtaacaacaa gatggtgagc
       61 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
      121 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      181 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
      241 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
      301 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      361 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccttgcagga cggcgagttc
      421 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
      481 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
      541 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
      601 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
      661 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
      721 ggccgccact ccaccggcgg catggacgag ctgtacaagt aataaatggt gagcaagggc
      781 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      841 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      901 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      961 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1021 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1081 gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga gttcatctac
     1141 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1201 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1261 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1321 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1381 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1441 cactccaccg gcggcatgga cgagctgtac aagtaataa
//