LOCUS       BBa_K784011              821 bp    DNA     linear       22-Dec-2024
DEFINITION  MCS+tac promoter+Spacer+RBS+mCherry
ACCESSION   BBa_K784011
VERSION     BBa_K784011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=MCS
     misc_feature    22..61
                     /label=tac promoter
     promoter        1..61
                     /label=BBa_K784008
     misc_feature    62..88
                     /label=Spacer region
     misc_feature    73..80
                     /label=PacI restriction site
     misc_feature    89..107
                     /label=RBS
     RBS             62..107
                     /label=BBa_K784007
     CDS             108..818
                     /label=mCherry
     misc_feature    459..459
                     /label=C->T (removing PstI site)
     misc_feature    108..821
                     /label=BBa_J06504
ORIGIN
        1 gagctcggta cccggggatc cgagctgttg acaattaatc atcggctcgt ataatgtgtg
       61 gtctctctct ctttaattaa tctctctcct gctaaggtaa caacaagatg gtgagcaagg
      121 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
      181 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      241 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
      301 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
      361 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      421 aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct
      481 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
      541 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
      601 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
      661 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
      721 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
      781 gccactccac cggcggcatg gacgagctgt acaagtaata a
//