LOCUS       BBa_K784014              828 bp    DNA     linear       22-Dec-2024
DEFINITION  pLux+Spacer2+RBS+mCherry
ACCESSION   BBa_K784014
VERSION     BBa_K784014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    64..95
                     /label=Spacer2
     RBS             96..114
                     /label=RBS
     misc_feature    80..87
                     /label=PacI restriction site
     RBS             64..114
                     /label=BBa_K784012
     CDS             115..825
                     /label=mCherry
     misc_feature    466..466
                     /label=C->T (removing PstI site)
     misc_feature    115..828
                     /label=BBa_J06504
     misc_feature    64..828
                     /label=BBa_K784009
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagtctctct ctctttagat taattaatct ctctcctgct aaggtaacaa caagatggtg
      121 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      181 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      241 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      301 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      361 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      421 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag
      481 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      541 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      601 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
      661 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
      721 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
      781 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaataa
//