BBa_K784027 1 BBa_K784027 XhoI+RBS+Alkaline Phosphatase (phoA) +XmaI 2012-09-16T11:00:00Z 2015-05-08T01:13:22Z the RBS and phoA part are from the parts registry this part consist of an XhoI restriction site, an RBS part, the phoA part and XmaI restriction site. false false _1039_ 0 11982 9 Not in stock false we made XhoI and XmaI sites on both sides in order to make ligation with this part false Lior Levy annotation2183982 1 RBS BioBrick B0064 range2183982 1 7 18 annotation2183981 1 phoA BioBrick J61032 range2183981 1 19 1493 annotation2183986 1 XmaI restriction site range2183986 1 1494 1499 annotation2183980 1 XhoI restriction site range2183980 1 1 6 BBa_K784026 1 BBa_K784026 SP6 promoter 2012-09-16T11:00:00Z 2015-05-08T01:13:22Z Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9. This part has the SP6 RNA Polymerase promoter false false _1039_ 0 11982 9 Not in stock false no particular considerations false Lior Levy BBa_K784030 1 pSP6+phoA pSP6+RBS+Alkaline Phosphatase (pHO)+Terminator 2012-09-16T11:00:00Z 2015-05-08T01:13:22Z The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9. The RBS is from the parts registry,BBa_B0064 made by Endi Lab (2008) The pHOA gene is from the parts registry,BBa_J61032 made by Arkin Lab (2006) This part consists of T7 RNA polymerase induced promoter followed by an RBS and the ‎Alkaline Phosphatase reporter gene (pHOA) from Citrobacter.‎ The part's purpose is to monitor the translation of the T7 RNA polymerase with the Alkaline ‎Phosphatase gene which can be quantified with the PNP (para-nitrophenol phosphate) hydrolysis assay that has been ‎made by Arkin Lab group in 2006.‎ The Alkaline Phosphatase + RBS part is between XmaI and XhoI restriction sites and ‎therefore can be excised out by these enzymes. false false _1039_ 0 11982 9 Not in stock false we used XhoI and XmaI restriction sites for the ligation because these sites are not anywhere in the sequence. false Lior Levy component2184056 1 BBa_K784027 component2184051 1 BBa_K784026 component2184057 1 BBa_K784002 annotation2184051 1 BBa_K784026 range2184051 1 1 17 annotation2184057 1 BBa_K784002 range2184057 1 1517 1563 annotation2184056 1 BBa_K784027 range2184056 1 18 1516 BBa_K784002 1 BBa_K784002 T7 Terminator 2012-09-12T11:00:00Z 2015-05-08T01:13:21Z http://nar.oxfordjournals.org/content/suppl/2012/06/05/gks597.DC1/nar-00461-c-2012-File007.pdf This part is a T7 RNAP Terminator, which can also be used for the following RNAP: T3,N4,K1F,SP6. false false _1039_ 0 11982 9 Not in stock false no particular design considerations false Lior Levy BBa_K784027_sequence 1 ctcgagaaagaggagaaaatgttttttattttaactgtatttatacatggagaaaataaagtgaaacaaagcgctatgatttttgccttgctgcctttgctgttggctccggccagtcaggcggacaccacaacaatatccgtgctggataatcgtgcggcgcagggcgatattactacgcccggcggcgcgcgccgattaaccggcgatcaaacggcggcgctgcgtgaatcgctaaatgataaaccggcgaaaaatattattctgctgatcggcgacggaatgggagattctgaaataaccgccgcacgaaattatgccgaaggcgccggcggcttttttaaaggtattgatgccctgccgctgaccggacaatatacccactatgcgctgaataaaaaaaccggcaaaccagattacgtgaccgattccgccgcctcagccaccgcgtggtccacaggggtgaaaacctacaacggcgcgctgggggtcgatattcatgaaaaagatcatctgaccattctcgaaatggcgaaggccgcaggtctggctaccggcaacgtctcgaccgcggagttgcaggacgccaccccggcagcgctgattgcccatgtcacctcgcgcaaatgctatggcccgacggcgaccagcgaaaaatgtccgacaaacgcgctggaaaagggcggcaaaggctcgattactgaacagctcctgaacgcgcgggcggacgtcaccctgggcggcggcgcgaaaaccttcgccgagacggcaaccgccggcgagtggcaggggaaaacgctgcgtgagcaggcgcagatgcgcggctaccagttagtcggcgatgccgcctctttagcggcgatcgacgaagcgaatcaggacaaaccgctgctgggactgttcgcagaggggaatatgccggtgcgctgggaagggccgaaggcctcgtaccacggcaatattgataaacctgccgtgacctgtacgccaaatccgaaacgcaatgacgcgattcccacgctggcgcagatgaccgacaaagccatcgaactgttgagcaaaaatgagcggggcttctttttacaggtggaaggcgcgtctatcgataagcaggatcacgccgcgaacccgtgcggacagattggcgagacggtggatcttgatgaagccgtacagagggcgctggcctttgcgaagaaagacggcaatacgctggtgatcgttaccgccgatcatgctcatgccagccagatcgtggcgcccgaaacgaaagcgccggggctgacgcaggcgctgaacaccaaagatggcgcggtgatggtcatcagctacggcaactcggaagaagattcccaggagcataccggcagccagctgcgcatcgccgcttacgggccgcatgcggcgaacgtggtcgggctgaccgatcaaaccgatctgttctacaccatgaaagcggcgctgggcctgaagtaatgccgcctctgggaattcccggg BBa_K784030_sequence 1 atttaggtgacactatactcgagaaagaggagaaaatgttttttattttaactgtatttatacatggagaaaataaagtgaaacaaagcgctatgatttttgccttgctgcctttgctgttggctccggccagtcaggcggacaccacaacaatatccgtgctggataatcgtgcggcgcagggcgatattactacgcccggcggcgcgcgccgattaaccggcgatcaaacggcggcgctgcgtgaatcgctaaatgataaaccggcgaaaaatattattctgctgatcggcgacggaatgggagattctgaaataaccgccgcacgaaattatgccgaaggcgccggcggcttttttaaaggtattgatgccctgccgctgaccggacaatatacccactatgcgctgaataaaaaaaccggcaaaccagattacgtgaccgattccgccgcctcagccaccgcgtggtccacaggggtgaaaacctacaacggcgcgctgggggtcgatattcatgaaaaagatcatctgaccattctcgaaatggcgaaggccgcaggtctggctaccggcaacgtctcgaccgcggagttgcaggacgccaccccggcagcgctgattgcccatgtcacctcgcgcaaatgctatggcccgacggcgaccagcgaaaaatgtccgacaaacgcgctggaaaagggcggcaaaggctcgattactgaacagctcctgaacgcgcgggcggacgtcaccctgggcggcggcgcgaaaaccttcgccgagacggcaaccgccggcgagtggcaggggaaaacgctgcgtgagcaggcgcagatgcgcggctaccagttagtcggcgatgccgcctctttagcggcgatcgacgaagcgaatcaggacaaaccgctgctgggactgttcgcagaggggaatatgccggtgcgctgggaagggccgaaggcctcgtaccacggcaatattgataaacctgccgtgacctgtacgccaaatccgaaacgcaatgacgcgattcccacgctggcgcagatgaccgacaaagccatcgaactgttgagcaaaaatgagcggggcttctttttacaggtggaaggcgcgtctatcgataagcaggatcacgccgcgaacccgtgcggacagattggcgagacggtggatcttgatgaagccgtacagagggcgctggcctttgcgaagaaagacggcaatacgctggtgatcgttaccgccgatcatgctcatgccagccagatcgtggcgcccgaaacgaaagcgccggggctgacgcaggcgctgaacaccaaagatggcgcggtgatggtcatcagctacggcaactcggaagaagattcccaggagcataccggcagccagctgcgcatcgccgcttacgggccgcatgcggcgaacgtggtcgggctgaccgatcaaaccgatctgttctacaccatgaaagcggcgctgggcctgaagtaatgccgcctctgggaattcccgggtagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K784026_sequence 1 atttaggtgacactata BBa_K784002_sequence 1 tagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z