BBa_K784033 1 BBa_K784033 pK1F_xyIE reporter gene 2012-09-17T11:00:00Z 2015-05-08T01:13:22Z The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh. The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9. This part's purpose is to testify to the presence of the K1F RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh (http://partsregistry.org/Part:BBa_J33204). It consists of an RNA K1F promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions. <br> The xyIE+RBS part is between XhoI and XmaI restriction sites, and therefore can be excised by these enzymes. false false _1039_ 0 12190 9 It's complicated false We had to make sure to use restriction enzymes that don't have a restriction site in the gene itself. false Noa Katz component2184552 1 BBa_K784031 component2184553 1 BBa_K784002 component2184551 1 BBa_K784025 annotation2184553 1 BBa_K784002 range2184553 1 1016 1062 annotation2184551 1 BBa_K784025 range2184551 1 1 23 annotation2184552 1 BBa_K784031 range2184552 1 32 1007 BBa_K784031 1 BBa_K784031 xhoI - xyIE - xmaI 2012-09-16T11:00:00Z 2015-05-08T01:13:22Z The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh. The part's name: BBa_J33204 The xyIE part with XhoI and XmaI at both ends. false false _1039_ 0 12190 9 Not in stock false We had to use retriction enzymes that don't appear in the xyIE gene. false Noa Katz BBa_K784002 1 BBa_K784002 T7 Terminator 2012-09-12T11:00:00Z 2015-05-08T01:13:21Z http://nar.oxfordjournals.org/content/suppl/2012/06/05/gks597.DC1/nar-00461-c-2012-File007.pdf This part is a T7 RNAP Terminator, which can also be used for the following RNAP: T3,N4,K1F,SP6. false false _1039_ 0 11982 9 Not in stock false no particular design considerations false Lior Levy BBa_K784025 1 BBa_K784025 K1F promoter 2012-09-16T11:00:00Z 2015-05-08T01:13:22Z Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9. This part has the K1F RNA Polymerase promoter false false _1039_ 0 11982 9 Not in stock false no particular considerations false Lior Levy BBa_K784025_sequence 1 taataactatcactatagggaga BBa_K784033_sequence 1 taataactatcactatagggagatactagagcgtctcgagctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctgatggtccggcccgggacgtactagagtagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K784002_sequence 1 tagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K784031_sequence 1 cgtctcgagctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctgatggtccggcccgggacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z