BBa_K784034 1 BBa_K784034 pSp6_xyIE reporter gene 2012-09-17T11:00:00Z 2015-05-08T01:13:22Z The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh. <br> The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9. This part's purpose is to testify to the presence of the Sp6 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh (http://partsregistry.org/Part:BBa_J33204). It consists of an RNA Sp6 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions. <br> The xyIE+RBS part is between XhoI and XmaI restriction sites, and therefore can be excised by these enzymes. false false _1039_ 0 12190 9 It's complicated false We had to make sure there aren't any restriction sites in the gene itself before using the XhoI and XmaI restriction enzymes. false Noa Katz component2184557 1 BBa_K784002 component2184556 1 BBa_K784031 component2184555 1 BBa_K784026 annotation2184556 1 BBa_K784031 range2184556 1 26 1001 annotation2184555 1 BBa_K784026 range2184555 1 1 17 annotation2184557 1 BBa_K784002 range2184557 1 1010 1056 BBa_K784026 1 BBa_K784026 SP6 promoter 2012-09-16T11:00:00Z 2015-05-08T01:13:22Z Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9. This part has the SP6 RNA Polymerase promoter false false _1039_ 0 11982 9 Not in stock false no particular considerations false Lior Levy BBa_K784031 1 BBa_K784031 xhoI - xyIE - xmaI 2012-09-16T11:00:00Z 2015-05-08T01:13:22Z The RBS and xyIE genes are from the parts registry, made by iGEM2006_Edinburgh. The part's name: BBa_J33204 The xyIE part with XhoI and XmaI at both ends. false false _1039_ 0 12190 9 Not in stock false We had to use retriction enzymes that don't appear in the xyIE gene. false Noa Katz BBa_K784002 1 BBa_K784002 T7 Terminator 2012-09-12T11:00:00Z 2015-05-08T01:13:21Z http://nar.oxfordjournals.org/content/suppl/2012/06/05/gks597.DC1/nar-00461-c-2012-File007.pdf This part is a T7 RNAP Terminator, which can also be used for the following RNAP: T3,N4,K1F,SP6. false false _1039_ 0 11982 9 Not in stock false no particular design considerations false Lior Levy BBa_K784026_sequence 1 atttaggtgacactata BBa_K784034_sequence 1 atttaggtgacactatatactagagcgtctcgagctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctgatggtccggcccgggacgtactagagtagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K784002_sequence 1 tagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K784031_sequence 1 cgtctcgagctcatgaactatgaagaggtgacgtcatgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgacctgatggtccggcccgggacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z