LOCUS       BBa_K784036             2908 bp    DNA     linear       30-Dec-2025
DEFINITION  N4 RNA polymerase + terminator
ACCESSION   BBa_K784036
VERSION     BBa_K784036.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     RBS             185..218
                     /label=RBS
     misc_feature    219..305
                     /label=proposed umu tag
     CDS             219..2993
                     /label=T7*(N4) RNAP
     CDS             138..2908
                     /label=BBa_K784035
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtgt tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt
      181 tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag attacgttga
      241 acagcgcatc gatctgggtg gcaacacgat taacatcgct aagaacgact tctctgacat
      301 cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg
      361 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat
      421 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat
      481 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc
      541 taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt
      601 agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt
      661 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat
      721 ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt
      781 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg
      841 tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt
      901 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc
      961 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat
     1021 cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc
     1081 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct
     1141 ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc
     1201 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt
     1261 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc
     1321 gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct
     1381 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg
     1441 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat
     1501 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc
     1561 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa
     1621 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg acaaggttcc
     1681 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa
     1741 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt
     1801 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct
     1861 ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt
     1921 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt
     1981 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt
     2041 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa
     2101 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt
     2161 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac
     2221 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc
     2281 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga
     2341 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa
     2401 gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc
     2461 tgtgtggcag gaatacaaga agcctattca gacgcgcatt gattgtgtta ttctgggtac
     2521 ccatcgtatg gctctgacca ttaacaccaa caaagatagc gagattgatg cacacaaaca
     2581 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac
     2641 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt
     2701 cggtacgatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga
     2761 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca
     2821 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga
     2881 catcttagag tcggacttcg cgttcgcg
//