LOCUS       BBa_K784037             2908 bp    DNA     linear       29-Jun-2024
DEFINITION  N4 RNA polymerase + terminator
ACCESSION   BBa_K784037
VERSION     BBa_K784037.1  
FEATURES             Location/Qualifiers
     RBS             48..81
                     /label=RBS
     misc_feature    82..168
                     /label=proposed umu tag
     CDS             82..2856
                     /label=T7*(N4) RNAP
     CDS             1..2771
                     /label=BBa_K784035
     stem_loop       2791..2834
                     /label=stem_loop
     misc_feature    2780..2859
                     /label=BBa_B0010
     terminator      2780..2859
                     /label=BBa_B0010
     stem_loop       2875..2894
                     /label=T7 TE
     misc_feature    2901..2901
                     /label=stop
     polyA_site      2895..2908
                     /label=polya
     misc_feature    2868..2908
                     /label=BBa_B0012
     terminator      2868..2908
                     /label=BBa_B0012
     terminator      2780..2908
                     /label=BBa_B0015
ORIGIN
        1 tgttgtttat caagcctgcg gatctccgcg aaattgtgac ttttccgcta tttagcgatc
       61 ttgttcagtg tggctttcct tcaccggcag cagattacgt tgaacagcgc atcgatctgg
      121 gtggcaacac gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc
      181 cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg
      241 agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta
      301 aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc ctactcccta
      361 agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc
      421 cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta
      481 agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg
      541 caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta
      601 agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac gtctacaaga
      661 aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg
      721 cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc
      781 tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag
      841 actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg
      901 cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg
      961 gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc
     1021 acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga
     1081 ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg
     1141 taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac
     1201 tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg
     1261 ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt
     1321 tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca
     1381 tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga
     1441 ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc
     1501 tgaaaatcca cggtgcaaac tgtgcgggtg tcgacaaggt tccgttccct gagcgcatca
     1561 agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca
     1621 cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg
     1681 gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt
     1741 gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta
     1801 acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg
     1861 agattctaca agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg
     1921 agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat
     1981 ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg
     2041 ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg
     2101 attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc
     2161 tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta
     2221 agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc
     2281 gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca
     2341 agaagcctat tcagacgcgc attgattgtg ttattctggg tacccatcgt atggctctga
     2401 ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc
     2461 ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg
     2521 agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacg attccggctg
     2581 acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg
     2641 atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca
     2701 aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact
     2761 tcgcgttcgc gtactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2821 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2881 ccttcgggtg ggcctttctg cgtttata
//