LOCUS BBa_K784037 2908 bp DNA linear 25-Nov-2024 DEFINITION N4 RNA polymerase + terminator ACCESSION BBa_K784037 VERSION BBa_K784037.1 FEATURES Location/Qualifiers RBS 48..81 /label=RBS misc_feature 82..168 /label=proposed umu tag CDS 82..2856 /label=T7*(N4) RNAP CDS 1..2771 /label=BBa_K784035 stem_loop 2791..2834 /label=stem_loop misc_feature 2780..2859 /label=BBa_B0010 terminator 2780..2859 /label=BBa_B0010 stem_loop 2875..2894 /label=T7 TE misc_feature 2901..2901 /label=stop polyA_site 2895..2908 /label=polya misc_feature 2868..2908 /label=BBa_B0012 terminator 2868..2908 /label=BBa_B0012 terminator 2780..2908 /label=BBa_B0015 ORIGIN 1 tgttgtttat caagcctgcg gatctccgcg aaattgtgac ttttccgcta tttagcgatc 61 ttgttcagtg tggctttcct tcaccggcag cagattacgt tgaacagcgc atcgatctgg 121 gtggcaacac gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc 181 cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg 241 agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta 301 aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc ctactcccta 361 agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc 421 cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta 481 agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg 541 caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta 601 agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac gtctacaaga 661 aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg 721 cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc atcgagatgc 781 tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag 841 actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg 901 cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg 961 gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc 1021 acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga 1081 ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg 1141 taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac 1201 tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg 1261 ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt 1321 tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca 1381 tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga 1441 ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc 1501 tgaaaatcca cggtgcaaac tgtgcgggtg tcgacaaggt tccgttccct gagcgcatca 1561 agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca 1621 cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg 1681 gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt 1741 gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta 1801 acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg 1861 agattctaca agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg 1921 agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat 1981 ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg 2041 ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg 2101 attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc 2161 tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta 2221 agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc 2281 gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca 2341 agaagcctat tcagacgcgc attgattgtg ttattctggg tacccatcgt atggctctga 2401 ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc 2461 ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg 2521 agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacg attccggctg 2581 acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg 2641 atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca 2701 aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact 2761 tcgcgttcgc gtactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 2821 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 2881 ccttcgggtg ggcctttctg cgtttata //