LOCUS       BBa_K784039             2962 bp    DNA     linear       26-Jun-2024
DEFINITION  T7*(K1F) RNA polymerase + terminator
ACCESSION   BBa_K784039
VERSION     BBa_K784039.1  
FEATURES             Location/Qualifiers
     RBS             3..21
                     /label=K1F rbs
     misc_feature    51..170
                     /label=proposed umo tag
     CDS             51..2825
                     /label=T7*(K1F) 
     CDS             1..2825
                     /label=BBa_K784038
     stem_loop       2845..2888
                     /label=stem_loop
     misc_feature    2834..2913
                     /label=BBa_B0010
     terminator      2834..2913
                     /label=BBa_B0010
     stem_loop       2929..2948
                     /label=T7 TE
     misc_feature    2955..2955
                     /label=stop
     polyA_site      2949..2962
                     /label=polya
     misc_feature    2922..2962
                     /label=BBa_B0012
     terminator      2922..2962
                     /label=BBa_B0012
     terminator      2834..2962
                     /label=BBa_B0015
ORIGIN
        1 ggaattgtga gcggataaca atttactaga taattgtgag cggataacaa atgttgttta
       61 tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt
      121 gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg ggtggcaaca
      181 cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc ccgttcaaca
      241 ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt gagcatgagt
      301 cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt aaagctggtg
      361 aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct aagatgattg
      421 cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc ccgacagcct
      481 tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt aagaccactc
      541 tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc gcaatcggtc
      601 gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct aagcacttca
      661 agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag aaagcattta
      721 tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag gcgtggtctt
      781 cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg ctcattgagt
      841 caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa gactctgaga
      901 ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt gcgctggctg
      961 gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact ggcattactg
     1021 gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact cacagtaaga
     1081 aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg attaacattg
     1141 cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac gtaatcacca
     1201 agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa ctcccgatga
     1261 aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt gctgccgctg
     1321 ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag ttcatgcttg
     1381 agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac atggactggc
     1441 gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg accaaaggac
     1501 tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg ctgaaaatcc
     1561 acggtgcaaa ctgtgcgggt gtcgacaagg ttccgttccc tgagcgcatc aagttcattg
     1621 aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac acttggtggg
     1681 ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct ggggtacagc
     1741 accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct tgctctggca
     1801 tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt aacttgcttc
     1861 ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac gagattctac
     1921 aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat gagaacactg
     1981 gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa tggctggctt
     2041 acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac gggtccaaag
     2101 agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt gattccggca
     2161 agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag ctgatttggg
     2221 aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt aagtctgctg
     2281 ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt cgcaagcgtt
     2341 gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac aagaagccta
     2401 ttcagacgcg cttgaacctg atgttcctcg gttcgttcaa cctccagccg accgtcaaca
     2461 ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct cctaactttg
     2521 tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac gagaagtacg
     2581 gaatcgaatc ttttgcactg attcacgact ccttcggtac gattccggct gacgctgcga
     2641 acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt gatgtactgg
     2701 ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac aaaatgccag
     2761 cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac ttcgcgttcg
     2821 cgtaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     2881 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     2941 ggtgggcctt tctgcgttta ta
//