LOCUS       BBa_K784042             2993 bp    DNA     linear       22-Dec-2024
DEFINITION  T7*(N4) polymerase + terminator
ACCESSION   BBa_K784042
VERSION     BBa_K784042.1  
FEATURES             Location/Qualifiers
     RBS             48..81
                     /label=RBS
     misc_feature    82..168
                     /label=proposed umu tag
     CDS             82..2856
                     /label=T7*(N4) RNAP
     CDS             1..2856
                     /label=BBa_K784035
     stem_loop       2876..2919
                     /label=stem_loop
     misc_feature    2865..2944
                     /label=BBa_B0010
     terminator      2865..2944
                     /label=BBa_B0010
     stem_loop       2960..2979
                     /label=T7 TE
     misc_feature    2986..2986
                     /label=stop
     polyA_site      2980..2993
                     /label=polya
     misc_feature    2953..2993
                     /label=BBa_B0012
     terminator      2953..2993
                     /label=BBa_B0012
     terminator      2865..2993
                     /label=BBa_B0015
ORIGIN
        1 tactagatgc agttttattc tctcgccagc actgtaatag gcactaatat ccaaaccagt
       61 agctcaattg gagtcgtcta tgtgttgttt atcaagcctg cggatctccg cgaaattgtg
      121 acttttccgc tatttagcga tcttgttcag tgtggctttc cttcaccggc agcagattac
      181 gttgaacagc gcatcgatct gggtggcaac acgattaaca tcgctaagaa cgacttctct
      241 gacatcgaac tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta
      301 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc
      361 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct
      421 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg
      481 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa
      541 gccgtagcgt acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca
      601 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt
      661 cgtatccgtg accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag
      721 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct
      781 aagggtctac tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta
      841 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa
      901 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag
      961 gctatcgcaa cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta
     1021 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt
     1081 cctctggcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac
     1141 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag
     1201 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc
     1261 cctgcgattg agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag
     1321 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag
     1381 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag
     1441 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc
     1501 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc
     1561 ggtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgacaag
     1621 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc
     1681 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt
     1741 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt
     1801 ccgctggcgt ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat
     1861 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg
     1921 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac
     1981 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc
     2041 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt
     2101 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa
     2161 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag
     2221 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg
     2281 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat
     2341 aagaagactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt
     2401 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcattgattg tgttattctg
     2461 ggtacccatc gtatggctct gaccattaac accaacaaag atagcgagat tgatgcacac
     2521 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt
     2581 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac
     2641 tccttcggta cgattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg
     2701 gttgacacat atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag
     2761 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc
     2821 cgtgacatct tagagtcgga cttcgcgttc gcgtaatact agagccaggc atcaaataaa
     2881 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2941 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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